Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F1 I2 R1 772 77.0 3769570 91.6% 3452926 105.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,215,740 A→G E88G (GAA→GGA)  aceB → malate synthase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,215,7400AG100.0% 276.6 / NA 78E88G (GAA→GGA) aceBmalate synthase A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/39);  total (39/39)

AACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGG  >  NC_000913/4215605‑4215873
                                                                                                                                       |                                                                                                                                     
aaCGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTaggaga                                                                                                                                    <  2:561556/139‑1 (MQ=255)
aaCGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTaggaga                                                                                                                                    >  1:473861/1‑139 (MQ=255)
  cGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGAcc                                                                                                                                  >  1:1735135/1‑139 (MQ=255)
   gCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGAccg                                                                                                                                 <  1:357847/139‑1 (MQ=255)
   gCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGAccg                                                                                                                                 <  2:576313/139‑1 (MQ=255)
            ttCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTaga                                                                                                                        >  1:1448393/1‑139 (MQ=255)
            ttCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTaga                                                                                                                        <  1:1613555/139‑1 (MQ=255)
             tCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTagag                                                                                                                       >  1:1614955/1‑139 (MQ=255)
               tGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCCGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGAccg                                                                                                                                 >  1:994127/1‑127 (MQ=255)
               tGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCCGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGAccg                                                                                                                                 <  2:994127/127‑1 (MQ=255)
                     cgcgcATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTgg                                                                                                               >  1:681238/1‑139 (MQ=255)
                            tCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTa                                                                                                        >  2:140402/1‑139 (MQ=255)
                              agcagcagcAAGATATTGATAACGGAACGTTGGCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGTGATAACTGGCCCGGt                                                                                                         <  1:1315802/136‑1 (MQ=255)
                              agcagcagcAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGt                                                                                                         >  2:1315802/1‑136 (MQ=255)
                              agcagcagcAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTaga                                                                                                      >  1:607524/1‑139 (MQ=255)
                                   cagcaAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCa                                                                                                 <  1:140402/139‑1 (MQ=255)
                                     gcaAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAg                                                                                               <  2:1381650/139‑1 (MQ=255)
                                      caAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGAccgc                                                                                                                                >  2:41826/1‑105 (MQ=255)
                                      caAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGAccgc                                                                                                                                <  1:41826/105‑1 (MQ=255)
                                      caAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAAc                                                                                                                  <  1:1707352/119‑1 (MQ=255)
                                      caAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAAc                                                                                                                  >  2:1707352/1‑119 (MQ=255)
                                                aTAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCaa                                                                                    >  2:304006/1‑139 (MQ=255)
                                                  aaCGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAAcg                                                                                  <  2:1735135/139‑1 (MQ=255)
                                                    cGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAgcg                                                                                                   <  2:387788/120‑1 (MQ=255)
                                                    cGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAgcg                                                                                                   >  1:387788/1‑120 (MQ=255)
                                                    cGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAAcgcg                                                                                >  1:1868746/1‑139 (MQ=255)
                                                    cGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAAcgcg                                                                                >  2:597059/1‑139 (MQ=255)
                                                    cGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAAcgcg                                                                                >  1:1523945/1‑139 (MQ=255)
                                                              ccTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCa                                                                      <  2:1868746/139‑1 (MQ=255)
                                                              ccTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCa                                                                      <  1:597059/139‑1 (MQ=255)
                                                              ccTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCa                                                                      >  1:528852/1‑139 (MQ=255)
                                                              ccTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCa                                                                      <  2:1523945/139‑1 (MQ=255)
                                                                tGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCcgcg                                                                                                                            >  2:1710618/1‑83 (MQ=255)
                                                                tGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCcgcg                                                                                                                            <  1:1710618/83‑1 (MQ=255)
                                                                 gATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAAtg                                                                   >  1:1017722/1‑139 (MQ=255)
                                                                   ttttATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAAtgtg                                                                 <  2:1448393/139‑1 (MQ=255)
                                                                      tATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTc                                                                             >  2:1601216/1‑124 (MQ=255)
                                                                      tATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTc                                                                             <  1:1601216/124‑1 (MQ=255)
                                                                      tATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGaaa                                                              <  2:607524/139‑1 (MQ=255)
                                                                            ggAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACcgccgc                                                                                                                             <  1:305418/70‑1 (MQ=255)
                                                                            ggAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACcgccgc                                                                                                                             >  2:305418/1‑70 (MQ=255)
                                                                            ggAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCttt                                                        >  1:848732/1‑139 (MQ=255)
                                                                                        cATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCa                                                                                                 >  1:1124759/1‑86 (MQ=255)
                                                                                        cATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCa                                                                                                 <  2:1124759/86‑1 (MQ=255)
                                                                                        cATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTc                                            >  2:395158/1‑139 (MQ=255)
                                                                                          ttCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAAc                                                                                   >  1:1665455/1‑98 (MQ=255)
                                                                                          ttCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAAc                                                                                   <  2:1665455/98‑1 (MQ=255)
                                                                                            cgcgATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAAtgtg                                                                 >  2:1422843/1‑114 (MQ=255)
                                                                                            cgcgATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAAtgtg                                                                 <  1:1422843/114‑1 (MQ=255)
                                                                                            cgcgATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAg                                        >  1:1834743/1‑139 (MQ=255)
                                                                                              cgATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAAc                                                                                                                  <  1:448107/63‑1 (MQ=255)
                                                                                              cgATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAAc                                                                                                                  >  2:448107/1‑63 (MQ=255)
                                                                                              cgATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCa                                                                                                 <  1:1829167/80‑1 (MQ=255)
                                                                                              cgATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCa                                                                                                 >  2:1829167/1‑80 (MQ=255)
                                                                                              cgATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGc                                                   >  1:577011/1‑126 (MQ=255)
                                                                                              cgATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGc                                                   <  2:577011/126‑1 (MQ=255)
                                                                                              cgATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGAt                                      <  2:473861/139‑1 (MQ=255)
                                                                                                  gCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCAc                                  <  2:1614955/139‑1 (MQ=255)
                                                                                                      aTTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAAc                                                                                                                  >  1:226187/1‑55 (MQ=255)
                                                                                                      aTTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAAc                                                                                                                  <  2:226187/55‑1 (MQ=255)
                                                                                                      aTTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCa                                                                                                 <  1:1052700/72‑1 (MQ=255)
                                                                                                      aTTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCa                                                                                                 >  2:1052700/1‑72 (MQ=255)
                                                                                                      aTTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGaaa                                                              <  1:557292/107‑1 (MQ=255)
                                                                                                      aTTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGaaa                                                              >  2:557292/1‑107 (MQ=255)
                                                                                                        tGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGTCGATTTCGAAGATTCACTGGCAc                            <  1:304006/139‑1 (MQ=255)
                                                                                                           aaaaTTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCAc                                  >  2:941758/1‑130 (MQ=255)
                                                                                                           aaaaTTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCAc                                  <  1:941758/130‑1 (MQ=255)
                                                                                                                tCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAAc                                                                                                                  <  1:1161103/45‑1 (MQ=255)
                                                                                                                tCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAAc                                                                                                                  >  2:1161103/1‑45 (MQ=255)
                                                                                                                tCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAAtgtg                                                                 >  1:337633/1‑94 (MQ=255)
                                                                                                                tCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAAtgtg                                                                 <  2:337633/94‑1 (MQ=255)
                                                                                                                tCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGc                                                   >  1:944212/1‑108 (MQ=255)
                                                                                                                tCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGc                                                   <  2:944212/108‑1 (MQ=255)
                                                                                                                              cGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACa                <  2:538011/129‑1 (MQ=255)
                                                                                                                              cGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACa                >  1:538011/1‑129 (MQ=255)
                                                                                                                              cGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGa      <  2:1017722/139‑1 (MQ=255)
                                                                                                                                gACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGAACCAGACTGGAACAAAGTGATCGACg    >  2:1043714/1‑139 (MQ=255)
                                                                                                                                  cTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCCCTGGCAc                            <  1:1229781/113‑1 (MQ=255)
                                                                                                                                  cTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCAc                            >  2:1229781/1‑113 (MQ=255)
                                                                                                                                  cTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACggg  <  2:681238/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                     
AACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGG  >  NC_000913/4215605‑4215873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A