Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F1 I1 R1
|
770 |
51.4 |
2439994 |
93.8% |
2288714 |
110.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,215,740 |
A→G |
E88G (GAA→GGA) |
aceB → |
malate synthase A |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,215,740 | 0 | A | G | 100.0%
| 106.9
/ NA
| 31 | E88G (GAA→GGA) | aceB | malate synthase A |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (12/19); total (12/19) |
CGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAAC > NC_000913/4215607‑4215858
|
cGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGAcc > 2:288187/1‑139 (MQ=255)
aaTAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGAccgcc < 2:1152934/139‑1 (MQ=255)
aaaCTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCcgcg < 1:251357/139‑1 (MQ=255)
cAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAAc < 2:796490/139‑1 (MQ=255)
cgcATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGcc < 2:351934/139‑1 (MQ=255)
aTTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCgg < 1:288187/139‑1 (MQ=255)
agcagcaAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAgcg < 1:929634/139‑1 (MQ=255)
cagcaAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCa < 1:155761/139‑1 (MQ=255)
gcaAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAg < 2:669133/139‑1 (MQ=255)
aGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAg > 2:944352/1‑136 (MQ=255)
aGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAg < 1:944352/136‑1 (MQ=255)
aaCGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTc > 2:350688/1‑139 (MQ=255)
tGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTagag < 2:628470/110‑1 (MQ=255)
tGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTagag > 1:628470/1‑110 (MQ=255)
ccTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCa < 1:350688/139‑1 (MQ=255)
ccTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCa < 2:83724/139‑1 (MQ=255)
tttCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACcgccgc > 2:1134719/1‑74 (MQ=255)
tttCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACcgccgc < 1:1134719/74‑1 (MQ=255)
cTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTa > 2:161862/1‑83 (MQ=255)
cTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTa < 1:161862/83‑1 (MQ=255)
cgATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAgcg < 1:799874/78‑1 (MQ=255)
cgATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAgcg > 2:799874/1‑78 (MQ=255)
cgATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGAt > 2:30737/1‑139 (MQ=255)
aTTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAAcgcg < 1:1046803/89‑1 (MQ=255)
aTTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAAcgcg > 2:1046803/1‑89 (MQ=255)
ttGGAAAATTCGCGGGATTTCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCa > 1:794230/1‑139 (MQ=255)
tCGCGGGATTCCTGCGGACTTAGGAGACCGCcgcg > 2:456070/1‑35 (MQ=255)
tCGCGGGATTCCTGCGGACTTAGGAGACCGCcgcg < 1:456070/35‑1 (MQ=255)
tCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGaa > 2:653226/1‑118 (MQ=255)
tCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGaa < 1:653226/118‑1 (MQ=255)
cgGGATTTCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAAc < 2:794230/139‑1 (MQ=255)
|
CGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAAC > NC_000913/4215607‑4215858
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
