Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F1 I2 R1 772 77.0 3769570 91.6% 3452926 105.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,268,809 C→T *68* (TAG→TAA)  yjbS ← uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,268,8090CT100.0% 171.4 / NA 53*68* (TAG→TAA) yjbSuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/25);  total (28/25)

TAAGTTTAACCTTATATTGATTACCAATATCAAATAAAAAGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTT  >  NC_000913/4268674‑4268943
                                                                                                                                       |                                                                                                                                      
tAAGTTTAACCTTATATTGATTACCAATATCAAATAAAAAGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGttaattaa                                                                                                                                     <  1:1245525/139‑1 (MQ=255)
        aCCTTATATTGATTACCAATATCAAATAAAAAGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTATTTCTTACCCCTGATTTTGTTAATTAAataaataa                                                                                                                             >  1:1000447/1‑139 (MQ=255)
          cTTATATTGATTACCAATATCAAATAAAAAGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAAt                                                                                                                           <  2:1394616/139‑1 (MQ=255)
                   aTTACCAATATCAAATAAAAAGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCCTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGaaaa                                                                                                                  >  1:992950/1‑139 (MQ=255)
                    ttACCAATATCAAATAAAAAGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGaaaaa                                                                                                                 >  1:1084483/1‑139 (MQ=255)
                      aCCAATATCAAATAAAAAGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACt                                                                                                               <  1:668749/139‑1 (MQ=255)
                           tatCAAATAAAAAGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACt                                                                                                          <  2:1084483/139‑1 (MQ=255)
                                           ccTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGAt                                                                                          >  2:692083/1‑139 (MQ=255)
                                           ccTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACGTGAt                                                                                          <  2:1000447/139‑1 (MQ=255)
                                           ccTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCCTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGAt                                                                                          <  2:992950/139‑1 (MQ=255)
                                             tGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGttaattaa                                                                                                                                     >  1:662151/1‑94 (MQ=255)
                                             tGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGttaattaa                                                                                                                                     <  2:662151/94‑1 (MQ=255)
                                                   gagaATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACttcttc                                                                                  >  2:931302/1‑139 (MQ=255)
                                                    agaATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATa                                                                                                   <  1:1774836/121‑1 (MQ=255)
                                                    agaATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATa                                                                                                   >  2:1774836/1‑121 (MQ=255)
                                                    agaATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTtcttct                                                                                 >  2:126167/1‑139 (MQ=255)
                                                      aataatCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACt                                                                                                          <  2:1224393/112‑1 (MQ=255)
                                                      aataatCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACt                                                                                                          >  1:1224393/1‑112 (MQ=255)
                                                         aatCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAgg                                                                            >  1:929003/1‑139 (MQ=255)
                                                           tCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTc                                                                          >  2:1544747/1‑139 (MQ=255)
                                                            cACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCt                                                                         >  2:1845771/1‑139 (MQ=255)
                                                                         ctcGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGttaattaa                                                                                                                                     >  1:31305/1‑66 (MQ=255)
                                                                         ctcGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGttaattaa                                                                                                                                     <  2:31305/66‑1 (MQ=255)
                                                                         ctcGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATActtc                                                                                     <  2:1829235/114‑1 (MQ=255)
                                                                         ctcGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATActtc                                                                                     >  1:1829235/1‑114 (MQ=255)
                                                                         ctcGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAACAATCTTTTg                                                            >  1:1110215/1‑139 (MQ=255)
                                                                         ctcGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTg                                                            <  1:692083/139‑1 (MQ=255)
                                                                                aaaCAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTg                                                            >  1:698553/1‑132 (MQ=255)
                                                                                aaaCAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTg                                                            <  2:698553/132‑1 (MQ=255)
                                                                                 aaCAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAAttttt                                                    <  2:929003/139‑1 (MQ=255)
                                                                                 aaCAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAAttttt                                                    <  1:931302/139‑1 (MQ=255)
                                                                                     gCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACt                                                <  1:1845771/139‑1 (MQ=255)
                                                                                       aaaGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCaa                                                                                                     <  2:1445737/84‑1 (MQ=255)
                                                                                       aaaGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCaa                                                                                                     >  1:1445737/1‑84 (MQ=255)
                                                                                       aaaGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACtgt                                              >  1:703455/1‑139 (MQ=255)
                                                                                       aaaGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACt                                                >  1:1850298/1‑137 (MQ=255)
                                                                                       aaaGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACt                                                <  2:1850298/137‑1 (MQ=255)
                                                                                             tGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCt                                        >  1:1809908/1‑139 (MQ=255)
                                                                                              gCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACtt                                                                                             <  1:630372/85‑1 (MQ=255)
                                                                                              gCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACtt                                                                                             >  2:630372/1‑85 (MQ=255)
                                                                                                     taCATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGttt                                          >  2:63011/1‑129 (MQ=255)
                                                                                                     taCATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGttt                                          <  1:63011/129‑1 (MQ=255)
                                                                                                     taCATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATaa                                >  2:1059187/1‑139 (MQ=255)
                                                                                                        aTAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAGTAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTTCAATTTTTGAc                                                 <  1:1088787/119‑1 (MQ=255)
                                                                                                        aTAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAAc                                                 >  2:1088787/1‑119 (MQ=255)
                                                                                                              tctttcttACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATc                       >  1:1873785/1‑139 (MQ=255)
                                                                                                                tttcttACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCtt                     <  2:1110215/139‑1 (MQ=255)
                                                                                                                tttcttACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCtt                     <  2:1873785/139‑1 (MQ=255)
                                                                                                                 ttcttACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACt                                                >  1:798456/1‑111 (MQ=255)
                                                                                                                 ttcttACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACt                                                <  2:798456/111‑1 (MQ=255)
                                                                                                                      aCCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACt                                                <  2:962431/106‑1 (MQ=255)
                                                                                                                      aCCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACt                                                >  1:962431/1‑106 (MQ=255)
                                                                                                                       ccccTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATa              <  1:1059187/139‑1 (MQ=255)
                                                                                                                        cccTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTa                                       >  1:970572/1‑113 (MQ=255)
                                                                                                                        cccTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTa                                       <  2:970572/113‑1 (MQ=255)
                                                                                                                        cccTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATaa             >  1:1101063/1‑139 (MQ=255)
                                                                                                                            gATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAgaga         >  1:1258015/1‑139 (MQ=255)
                                                                                                                               tttGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGatat      <  1:126167/139‑1 (MQ=255)
                                                                                                                                   ttaattaaATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCttt  <  2:703455/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
TAAGTTTAACCTTATATTGATTACCAATATCAAATAAAAAGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTT  >  NC_000913/4268674‑4268943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A