Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I2 R1	757	38.1	1607544	96.5%	1551279	117.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,279,634	G→A	V386I (GTT→ATT)	ghxP →	guanine/hypoxanthine permease, high affinity, guanine/hypoxanthine:H+ symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,279,634	0	G	A	100.0%	128.7 / NA	41	V386I (GTT→ATT)	ghxP	guanine/hypoxanthine permease, high affinity, guanine/hypoxanthine:H+ symporter
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (24/17); total (24/17)

GTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTG  >  NC_000913/4279507‑4279762

gTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATg                                                                                                                       >  2:82303/1‑139 (MQ=255)

 ttCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATgg                                                                                                                      <  2:622258/139‑1 (MQ=255)

                ttCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACgg                                                                                                       >  1:451226/1‑139 (MQ=255)

                 tCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGc                                                                                                      >  2:736769/1‑139 (MQ=255)

                     ctctcCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTa                                                                                                  >  2:741985/1‑139 (MQ=255)

                        tcCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTc                                                                                               >  1:767259/1‑139 (MQ=255)

                         ccGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAACTCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCa                                                                                              <  1:721841/139‑1 (MQ=255)

                             tctctTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGt                                                                                          >  2:274462/1‑139 (MQ=255)

                                 ttACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTg                                                                                      <  1:181620/139‑1 (MQ=255)

                                       cGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTg                                                                                         <  2:739095/130‑1 (MQ=255)

                                       cGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTg                                                                                         >  1:739095/1‑130 (MQ=255)

                                         ttCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCt                                                                                       <  2:96205/130‑1 (MQ=255)

                                         ttCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCt                                                                                       >  1:96205/1‑130 (MQ=255)

                                         ttCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTaa                                                                              >  1:597659/1‑139 (MQ=255)

                                         ttCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTaa                                                                              >  1:102866/1‑139 (MQ=255)

                                         ttCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTaa                                                                              >  2:237110/1‑139 (MQ=255)

                                           ccGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACa                                                                            <  2:597659/139‑1 (MQ=255)

                                                 tATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTaa                                                                      <  2:102866/139‑1 (MQ=255)

                                                        cGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGcat                                                               >  1:664861/1‑139 (MQ=255)

                                                           cTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGt                                                                                                            <  1:394706/91‑1 (MQ=255)

                                                           cTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGt                                                                                                            >  2:394706/1‑91 (MQ=255)

                                                             ccGGCGCTGATGTACGTTCGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACACGCATCATGa                                                          <  2:540742/139‑1 (MQ=255)

                                                                gcgcTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCg                                                       >  2:42276/1‑139 (MQ=255)

                                                                      aTGTACGTTGTGCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCTATGGTTGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATTATGATCGGCTTcg                                                 <  1:541479/139‑1 (MQ=255)

                                                                           cGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTg                                                                                      <  2:26153/97‑1 (MQ=255)

                                                                           cGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTg                                                                                      >  1:26153/1‑97 (MQ=255)

                                                                                gCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGTCGGGTCTGGTTAGGGTTGTATTCATCGTGCTGACCTGTCACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGt                                       >  2:234010/1‑139 (MQ=255)

                                                                                gCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGt                                       >  2:613496/1‑139 (MQ=255)

                                                                                gCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGAAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGt                                       >  2:228466/1‑139 (MQ=255)

                                                                                              aGCAACGTGGCGAAAATCGATTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTGGTCGTCTgg                         >  1:472342/1‑139 (MQ=255)

                                                                                              aGCAACGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTGGTCGTCTgg                         >  1:505803/1‑139 (MQ=255)

                                                                                                   cGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGt                                                                                <  2:588112/79‑1 (MQ=255)

                                                                                                   cGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGt                                                                                >  1:588112/1‑79 (MQ=255)

                                                                                                   cGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTaa                                                                              >  1:60596/1‑81 (MQ=255)

                                                                                                   cGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTaa                                                                              <  2:60596/81‑1 (MQ=255)

                                                                                                   cGTGGCGAAAATCGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTGGTCGTCTGGTTTcc                    >  1:230036/1‑139 (MQ=255)

                                                                                                               cGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGgcgc        <  2:767259/139‑1 (MQ=255)

                                                                                                               cGACTTTGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGgcgc        <  1:741985/139‑1 (MQ=255)

                                                                                                                     tGCTGATTTTATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTg  <  2:505803/139‑1 (MQ=255)

                                                                                                                           ttttATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGgcgc        <  2:56426/127‑1 (MQ=255)

                                                                                                                           ttttATTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGgcgc        >  1:56426/1‑127 (MQ=255)

GTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTG  >  NC_000913/4279507‑4279762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A