Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I2 R1	772	77.0	3769570	91.6%	3452926	105.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,606,330	(G)_8→9	noncoding (72/87 nt)	leuQ ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,606,322	1	.	G	86.2%	168.0 / 19.1	58	noncoding (80/87 nt)	leuQ	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base . (5/3); new base G (25/25); total (30/28)
Fisher's exact test for biased strand distribution p-value = 7.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑A‑‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGT  >  NC_000913/4606187‑4606453

atatCGCCTCGTTTGGTGCGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑A‑‑g‑g‑g                                                                                                                                  <  2:1871607/139‑1 (MQ=255)

          gTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑A‑‑g‑g‑gggggggactt                                                                                                                        >  2:185863/1‑134 (MQ=255)

             tGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGaa                                                                                                                      <  1:185863/139‑1 (MQ=255)

               gTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAAcc                                                                                                                    >  1:1379709/1‑139 (MQ=255)

               gTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑A‑‑g‑g‑ggggggggacttg                                                                                                                      >  2:1529653/1‑129 (MQ=255)

               gTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGAAATATCAACGTGGTTTTTGGTGCG‑A‑‑g‑g‑ggggggggacttg                                                                                                                      <  1:1529653/136‑8 (MQ=255)

                tGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGCG‑‑G‑G‑GGGGGGACTGGAAccc                                                                                                                   >  2:916210/1‑139 (MQ=18)

                    aGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑A‑‑g                                                                                                                                      >  1:1128842/1‑117 (MQ=255)

                    aGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑A‑‑g                                                                                                                                      <  2:1128842/117‑1 (MQ=255)

                     ggggggggACTTGAACCCCCACGTCCGTAAGGACACTATCACCTGAAGCTGGCGCCTCTACCAATTCCGCCACCTTCGCCCACTGTGAGCAATATCAACGGGTTTTTTTGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACg                                                                                                              <  1:967437/139‑1 (MQ=18)

                     ggggggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACg                                                                                                              <  2:311405/139‑1 (MQ=255)

                          gggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGt                                                                                                         >  1:1170790/1‑139 (MQ=255)

                          gggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGt                                                                                                         >  2:969750/1‑139 (MQ=255)

                               ttGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGa                                                                                                    <  1:969750/139‑1 (MQ=255)

                                   aCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                                                                                                <  2:1379709/139‑1 (MQ=255)

                                       ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑A‑‑g                                                                                                                                      >  2:1844201/1‑98 (MQ=255)

                                       ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑A‑‑g                                                                                                                                      <  1:1844201/98‑1 (MQ=255)

                                            tCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAAcccc                                                                                                                  >  2:603695/1‑112 (MQ=255)

                                            tCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAAcccc                                                                                                                  <  1:603695/112‑1 (MQ=255)

                                                       acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg                                                                                 >  2:488569/1‑134 (MQ=255)

                                                       acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg                                                                                 <  1:488569/134‑1 (MQ=255)

                                                       acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGt                                                                            >  2:1749012/1‑139 (MQ=255)

                                                          aaCACCTGAAGCTAGCGCGTCTACCAATTGCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑A‑‑G‑GCGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTa                                                                         >  1:1083580/1‑139 (MQ=255)

                                                               cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAt                                                                    >  2:1429154/1‑139 (MQ=255)

                                                                 gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGaca                                                                                                  >  2:1407437/1‑107 (MQ=255)

                                                                 gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGaca                                                                                                  <  1:1407437/107‑1 (MQ=255)

                                                                      tAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                               >  2:1769967/1‑137 (MQ=255)

                                                                      tAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACGTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                               <  1:1769967/137‑1 (MQ=255)

                                                                         cgcgTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                                                          >  1:1580775/1‑139 (MQ=255)

                                                                          gcgTCTACCAATCCCGCCCCCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt                                                         <  2:1750559/139‑1 (MQ=255)

                                                                             tCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc                                                      >  1:1504134/1‑139 (MQ=255)

                                                                              cTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTTAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCa                                                     <  2:1170790/139‑1 (MQ=255)

                                                                                      tCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                                                                                                >  1:1556827/1‑88 (MQ=255)

                                                                                      tCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                                                                                                <  2:1556827/88‑1 (MQ=255)

                                                                                       ccGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGCACACTAACACCTGAAGCTAGCGCGTCTACCAAt                                                                    <  2:1838573/115‑1 (MQ=255)

                                                                                       ccGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGCACACTAACACCTGAAGCTAGCGCGTCTACCAAt                                                                    >  1:1838573/1‑115 (MQ=255)

                                                                                        cGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa                                                                                              <  2:1813918/88‑1 (MQ=255)

                                                                                        cGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa                                                                                              >  1:1813918/1‑88 (MQ=255)

                                                                                          ccACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTc                                         >  2:1095856/1‑139 (MQ=255)

                                                                                               ttCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                      <  2:985225/105‑1 (MQ=255)

                                                                                               ttCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                      >  1:985225/1‑105 (MQ=255)

                                                                                                        tGCGTGCAATATCAACGTGGTTTTTGGTGCG‑A‑‑g‑g‑g                                                                                                                                  >  2:1384092/1‑35 (MQ=255)

                                                                                                        tGCGAGCAATATCAACGTGGTTTTTGGTGGG‑A‑‑g‑g‑g                                                                                                                                  <  1:1384092/35‑1 (MQ=255)

                                                                                                        tGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AGGG‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                                                             <  2:777936/106‑1 (MQ=255)

                                                                                                        tGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AGGG‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                                                             >  1:777936/1‑106 (MQ=255)

                                                                                                        tGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                                                             >  2:1639900/1‑105 (MQ=255)

                                                                                                        tGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                                                             <  1:1639900/105‑1 (MQ=255)

                                                                                                        tGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCAt                                               >  1:756559/1‑119 (MQ=255)

                                                                                                        tGCGAGCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCAt                                               <  2:756559/119‑1 (MQ=255)

                                                                                                             gCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCa                                                     <  1:322171/108‑1 (MQ=255)

                                                                                                             gCAATATCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCa                                                     >  2:322171/1‑108 (MQ=255)

                                                                                                               aaTATCAATGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACg                    <  2:629800/139‑1 (MQ=255)

                                                                                                               aaTATCAACGTGATTTTTGGTGCG‑A‑‑GAG‑GGGGGAACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTATCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACg                    <  1:1749012/139‑1 (MQ=21)

                                                                                                                   tCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc                                                            <  2:1764940/95‑1 (MQ=255)

                                                                                                                   tCAACGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc                                                            >  1:1764940/1‑95 (MQ=255)

                                                                                                                    cAACGTGGTTTTTGGTGCG‑A‑‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg              <  2:1298191/139‑1 (MQ=255)

                                                                                                                     aaCGTGGTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg              <  2:1810821/139‑1 (MQ=255)

                                                                                                                           gTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg                                                                                 >  1:943505/1‑66 (MQ=255)

                                                                                                                           gTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg                                                                                 <  2:943505/66‑1 (MQ=255)

                                                                                                                           gTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaaaa                                   >  1:805818/1‑112 (MQ=255)

                                                                                                                           gTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaaaa                                   <  2:805818/112‑1 (MQ=255)

                                                                                                                           gTTTTTGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCt        >  2:275486/1‑139 (MQ=255)

                                                                                                                           gTTTTTGCTGCGCG‑‑G‑G‑GGGGGGGCTTGGAGCCCCACGTCCGCAAGCACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGTATACCCTCAATTCCTAAAGAGAATTGCTACCACGGCGTAGCATTCt        >  1:1302435/1‑139 (MQ=17)

                                                                                                                            tttttGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaaaa                                   >  1:1432303/1‑111 (MQ=255)

                                                                                                                            tttttGGTGCG‑AG‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaaaa                                   <  2:1432303/111‑1 (MQ=255)

                                                                                                                              tttGGTGCG‑A‑‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                                                                                                >  2:315500/1‑47 (MQ=32)

                                                                                                                              tttGGTGCG‑A‑‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                                                                                                <  1:315500/47‑1 (MQ=32)

                                                                                                                                tGGTGCG‑A‑‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATAGTATCAATTCTTTAAAAGAATTGCTACCACTGAGGCGGATTGTAGTgac  >  1:1298191/1‑137 (MQ=17)

ATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCG‑A‑‑G‑G‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGT  >  NC_000913/4606187‑4606453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A