Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I1 R1
|
781 |
46.9 |
2231650 |
93.5% |
2086592 |
109.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,606,330 |
(G)8→9 |
noncoding (72/87 nt) |
leuQ ← |
tRNA‑Leu |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,606,322 | 1 | . | G | 86.1%
| 99.3
/ 11.0
| 36 | noncoding (80/87 nt) | leuQ | tRNA‑Leu |
| Reads supporting (aligned to +/- strand): ref base . (3/2); new base G (16/15); total (19/17) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01 |
TTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTC > NC_000913/4606184‑4606446
|
ttGATATCGCCTCGTTTGGTGGGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa < 1:584977/139‑1 (MQ=255)
ttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCTAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa > 1:713687/1‑139 (MQ=255)
ttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa > 1:137611/1‑139 (MQ=255)
tttGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑gggg‑ggggggcttg > 1:155310/1‑133 (MQ=255)
gggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGt > 2:520528/1‑139 (MQ=255)
gggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGCGGGGTCTTGAACCCCCACGTCCGt > 1:216414/1‑139 (MQ=255)
tGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGTGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGac < 2:713687/139‑1 (MQ=255)
gAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAAcccc < 1:179664/123‑1 (MQ=255)
gAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAAcccc > 2:179664/1‑123 (MQ=255)
gAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑gggg‑gggggac > 1:765370/1‑111 (MQ=255)
gAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑gggg‑gggggac < 2:765370/114‑4 (MQ=255)
gAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAACAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGTCTTGAACCCCCACGTCCGTAAGTaca > 2:926571/1‑139 (MQ=21)
aCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACt < 1:520528/139‑1 (MQ=255)
ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAaca > 1:711668/1‑139 (MQ=255)
gacacTAACTCCTGAAGCTAGCGCCTCTACCAATTCCGCAACCGACGCCCAGTGCGATTATTAGAAACGGGGTTTTTGGGGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcg < 2:137611/138‑1 (MQ=18)
acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCa > 1:797768/1‑103 (MQ=255)
acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCa < 2:797768/103‑1 (MQ=255)
ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAt > 2:86770/1‑139 (MQ=255)
aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg < 1:548432/139‑1 (MQ=255)
cgcgTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt > 2:739844/1‑139 (MQ=255)
tCTACCAATTCCGCCACCTTCGCCCAGTGCGATCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGTCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc > 2:548432/1‑139 (MQ=255)
tCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAaca > 1:946561/1‑101 (MQ=255)
tCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAaca < 2:946561/101‑1 (MQ=255)
tcctaTTCCGCCACGTTCGCTCAGTGCGAGAAACATCAACGGCGTTGTTGGGGGGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATa < 2:155310/135‑1 (MQ=14)
ccACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTc > 1:235304/1‑139 (MQ=255)
cACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc > 1:204193/1‑125 (MQ=255)
cACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc < 2:204193/125‑1 (MQ=255)
tGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACg > 2:957937/1‑56 (MQ=25)
tGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACg < 1:957937/56‑1 (MQ=25)
aGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACg < 2:526113/52‑1 (MQ=25)
aGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACg > 1:526113/1‑52 (MQ=25)
aGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCaa < 2:1088970/118‑1 (MQ=255)
aGCAATATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCaa > 1:1088970/1‑118 (MQ=255)
aaTATCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACg < 1:739844/139‑1 (MQ=255)
atCAACGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAg > 2:1069315/1‑139 (MQ=255)
aaCGTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg < 2:235304/139‑1 (MQ=255)
gTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg > 2:504619/1‑69 (MQ=255)
gTGGTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg < 1:504619/69‑1 (MQ=255)
tGGTTTTTGGTGCGA‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTc < 1:86770/139‑1 (MQ=255)
gTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCaa < 1:949784/103‑1 (MQ=255)
gTTTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCaa > 2:949784/1‑103 (MQ=255)
gTTTTTGGTGCGA‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAAt > 1:83482/1‑116 (MQ=255)
gTTTTTGGTGCGA‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAAt < 2:83482/116‑1 (MQ=255)
|
TTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTC > NC_000913/4606184‑4606446
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
