Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F1 I1 R1 776 60.0 3074904 90.1% 2770488 104.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 401,548 (G)5→4 coding (163/261 nt) iraP → anti‑RssB factor, RpoS stabilzer during Pi starvation, anti‑adapter protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913401,5440G.100.0% 186.7 / NA 45coding (159/261 nt)iraPanti‑RssB factor, RpoS stabilzer during Pi starvation, anti‑adapter protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (23/22);  total (23/22)

AGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAA  >  NC_000913/401417‑401676
                                                                                                                               |                                                                                                                                    
aGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAgg                                                                                                                                     <  1:895596/129‑1 (MQ=255)
aGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAAAAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTAc                                                                                                                          >  2:502147/1‑139 (MQ=255)
aGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTAACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAgg                                                                                                                                     >  2:895596/1‑129 (MQ=255)
     gCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGt                                                                                                                     >  2:452110/1‑139 (MQ=255)
      cccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTa                                                                                                                    >  2:1477690/1‑139 (MQ=255)
      cccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTa                                                                                                                    >  1:844381/1‑139 (MQ=255)
      cccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTa                                                                                                                    >  1:1330405/1‑139 (MQ=255)
              gaagaagaGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGa                                                                                                            <  2:1330405/139‑1 (MQ=255)
                      gTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCa                                                                                                    <  1:205745/139‑1 (MQ=255)
                       tCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCAt                                                                                                   >  2:366020/1‑139 (MQ=255)
                                 tgtgtgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTgacgac                                                                                         >  2:1250263/1‑139 (MQ=255)
                                   tgtgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGAcgacga                                                                                       >  2:234798/1‑139 (MQ=255)
                                     tgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATa                                                                                     <  2:844381/139‑1 (MQ=255)
                                     tgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATa                                                                                     <  1:366020/139‑1 (MQ=255)
                                             tAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAgctg                                                                             >  2:49194/1‑139 (MQ=255)
                                                  gCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTgcg                                                                        <  1:192684/139‑1 (MQ=255)
                                                     ttGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGa                                                                     <  1:452110/139‑1 (MQ=255)
                                                      tGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAggggcg                                                                                                                                 <  1:909307/79‑3 (MQ=255)
                                                      tGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAggggcg                                                                                                                                 >  2:909307/1‑77 (MQ=255)
                                                          gaTTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTAc                                                                                                                          >  1:30517/1‑81 (MQ=255)
                                                          gaTTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTAc                                                                                                                          <  2:30517/81‑1 (MQ=255)
                                                            ttATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTa                                                              <  2:1240781/139‑1 (MQ=255)
                                                            ttATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTa                                                              >  2:941648/1‑139 (MQ=255)
                                                             tATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGa                                                                     <  1:1147305/131‑1 (MQ=255)
                                                             tATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCAGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGa                                                                     >  2:1147305/1‑131 (MQ=255)
                                                              aTCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTaaa                                                            <  2:184647/139‑1 (MQ=255)
                                                                    aCTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGGTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGtt                                                      <  1:941648/139‑1 (MQ=255)
                                                                     cTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTa                                                     <  1:49194/139‑1 (MQ=255)
                                                                         aaTGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGa                                                 >  1:601701/1‑139 (MQ=255)
                                                                              gtCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGCAGTTATTGAAGCAt                                            >  1:943710/2‑139 (MQ=255)
                                                                                cGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAtt                                                                   <  1:1263489/114‑1 (MQ=255)
                                                                                cGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAtt                                                                   >  2:1263489/1‑114 (MQ=255)
                                                                                cGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATcc                                          >  2:82525/1‑139 (MQ=255)
                                                                                  cAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAaag                                                           >  2:513006/1‑120 (MQ=255)
                                                                                  cAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAaag                                                           <  1:513006/120‑1 (MQ=255)
                                                                                    atatGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGGGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGt                                      <  2:943710/139‑1 (MQ=255)
                                                                                       tGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAggggcg                                                                                                                                 <  1:727178/46‑3 (MQ=255)
                                                                                       tGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAggggcg                                                                                                                                 >  2:727178/1‑44 (MQ=255)
                                                                                         gcgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTaa                                                             >  2:776342/1‑111 (MQ=255)
                                                                                         gcgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTaa                                                             <  1:776342/111‑1 (MQ=255)
                                                                                          cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGAggggcg                                                                                                                                 <  2:974092/43‑3 (MQ=255)
                                                                                          cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGAggggcg                                                                                                                                 >  1:974092/1‑41 (MQ=255)
                                                                                          cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGTCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATc                                           >  1:1046076/1‑128 (MQ=255)
                                                                                          cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGTTGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATc                                           <  2:1046076/128‑1 (MQ=255)
                                                                                          cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATg                                                                                                          <  2:299178/65‑1 (MQ=255)
                                                                                          cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATg                                                                                                          >  1:299178/1‑65 (MQ=255)
                                                                                          cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTgacga                                                                                          <  2:313681/81‑1 (MQ=255)
                                                                                          cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTgacga                                                                                          >  1:313681/1‑81 (MQ=255)
                                                                                          cacAAAATGACCAACAGCGGTTGATTGATCAGGTAGAggggcg                                                                                                                                 >  2:30596/3‑41 (MQ=255)
                                                                                          cacAAAATGACCAACAGCGGTTGATTGATCAGGTAGAggggcg                                                                                                                                 <  1:30596/41‑3 (MQ=255)
                                                                                               aaTGACCAACAGCGGTTGATTGATCAGGTAGAggggcg                                                                                                                                 <  2:1352477/38‑3 (MQ=255)
                                                                                               aaTGACCAACAGCGGTTGATTGATCAGGTAGAggggcg                                                                                                                                 >  1:1352477/1‑36 (MQ=255)
                                                                                                      aaCAGCGGTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGTCGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCtt                    <  1:718684/139‑1 (MQ=255)
                                                                                                             gTTGATTGATCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACa             >  1:566962/1‑139 (MQ=255)
                                                                                                              ttgattgaTCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAg            <  1:1477690/139‑1 (MQ=255)
                                                                                                                    gatCAGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCa      <  1:234798/139‑1 (MQ=255)
                                                                                                                        aGGTAGA‑GGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATaaa  >  1:325350/1‑139 (MQ=255)
                                                                                                                               |                                                                                                                                    
AGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGGGGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAA  >  NC_000913/401417‑401676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A