Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I1 R1	770	68.5	3470224	91.7%	3182195	103.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	405,224	G→A	P141S (CCA→TCA)	proC ←	pyrroline‑5‑carboxylate reductase, NAD(P)‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	405,224	0	G	A	100.0%	132.4 / NA	42	P141S (CCA→TCA)	proC	pyrroline‑5‑carboxylate reductase, NAD(P)‑binding
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (24/18); total (24/18)

GCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGC  >  NC_000913/405115‑405353

gCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAgg                                                                                                      >  2:267704/1‑139 (MQ=255)

      gaagaaCCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATcc                                                                                                <  2:1733836/139‑1 (MQ=255)

          aaCCGCTCACACCGACCACTGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTgg                                                                                                                 >  2:1635774/1‑118 (MQ=255)

          aaCCGCTCACACCGACCACTGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTgg                                                                                                                 <  1:1635774/118‑1 (MQ=255)

          aaCCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGc                                                                                            >  1:944108/1‑139 (MQ=255)

          aaCCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGc                                                                                            >  1:339196/1‑139 (MQ=255)

          aaCCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTAAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGc                                                                                            >  2:1241369/1‑139 (MQ=255)

               cTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTaa                                                                                       >  1:1553993/1‑139 (MQ=255)

                tCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAAc                                                                                      <  1:1241369/139‑1 (MQ=255)

                      cGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGt                                                                                                     <  2:1472819/118‑1 (MQ=255)

                      cGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGt                                                                                                     >  1:1472819/1‑118 (MQ=255)

                         ccaccGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACg                                                                                                          <  1:657120/110‑1 (MQ=255)

                         ccaccGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACg                                                                                                          >  2:657120/1‑110 (MQ=255)

                         ccaccGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAAc                                                                                      >  1:1705262/1‑130 (MQ=255)

                         ccaccGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAAc                                                                                      <  2:1705262/130‑1 (MQ=255)

                                       atcGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATgg                                                               >  2:848474/1‑139 (MQ=255)

                                          ggCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGgcgc                                                            >  2:435915/1‑139 (MQ=255)

                                             tCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGACATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGa                                                         <  2:508509/139‑1 (MQ=255)

                                               aGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATa                                                       >  1:1423605/1‑139 (MQ=255)

                                               aGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATa                                                       >  1:608131/1‑139 (MQ=255)

                                                 cAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAAt                                                     >  2:650560/1‑139 (MQ=255)

                                                           cGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCa                                           >  2:712055/1‑139 (MQ=255)

                                                              ttCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATgg                                        >  1:962777/1‑139 (MQ=255)

                                                                      agcagcGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAgcgc                                <  2:1423605/139‑1 (MQ=255)

                                                                       gcagcGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAgcgcg                               >  1:653824/1‑139 (MQ=255)

                                                                          gcGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCgg                            >  1:1312100/1‑139 (MQ=255)

                                                                           cGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAg                                                                                                       <  2:670659/63‑1 (MQ=255)

                                                                           cGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAg                                                                                                       >  1:670659/1‑63 (MQ=255)

                                                                            ggAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCAt                                                                                          >  2:1070407/1‑75 (MQ=255)

                                                                            ggAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCAt                                                                                          <  1:1070407/75‑1 (MQ=255)

                                                                                aaTATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGc                      >  2:73026/1‑139 (MQ=255)

                                                                                    ttCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGt                  <  2:944108/139‑1 (MQ=255)

                                                                                    ttCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGt                  <  1:848474/139‑1 (MQ=255)

                                                                                    ttCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATAGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGt                  <  2:339196/139‑1 (MQ=255)

                                                                                         cacaTCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGAt                                                        <  2:158234/96‑1 (MQ=255)

                                                                                         cacaTCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGAt                                                        >  1:158234/1‑96 (MQ=255)

                                                                                            aTCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTg          >  2:1649639/1‑139 (MQ=255)

                                                                                                 cGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATg                                                                >  2:647689/1‑80 (MQ=255)

                                                                                                 cGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATg                                                                <  1:647689/80‑1 (MQ=255)

                                                                                                 cGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACAcc     <  1:73026/139‑1 (MQ=255)

                                                                                                    tATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTgc  <  2:1312100/139‑1 (MQ=255)

                                                                                                    tATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTgc  <  1:267704/139‑1 (MQ=255)

GCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGC  >  NC_000913/405115‑405353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A