Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	405,224	G→A	P141S (CCA→TCA)	proC ←	pyrroline‑5‑carboxylate reductase, NAD(P)‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	405,224	0	G	A	100.0%	106.3 / NA	34	P141S (CCA→TCA)	proC	pyrroline‑5‑carboxylate reductase, NAD(P)‑binding
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (15/19); total (15/19)

AACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAA  >  NC_000913/405100‑405358

aaCATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGt                                                                                                                          <  1:953270/139‑1 (MQ=255)

         aCGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTa                                                                                                                 <  1:941348/139‑1 (MQ=255)

             aGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGa                                                                                                             >  1:207440/1‑139 (MQ=255)

                cTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGt                                                                                                          <  1:244752/139‑1 (MQ=255)

                   gCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCAt                                                                                                       >  2:819925/1‑139 (MQ=255)

                     gaagaaCCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATcc                                                                                                     <  1:110097/139‑1 (MQ=255)

                     gaagaaCCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATcc                                                                                                     <  2:322809/139‑1 (MQ=255)

                     gaagaaCCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATcc                                                                                                     <  1:842629/139‑1 (MQ=255)

                         aaCCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGc                                                                                                 >  1:643632/1‑139 (MQ=255)

                              cTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTaa                                                                                            >  2:141721/1‑139 (MQ=255)

                                       accaccGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCgg                                                                                   >  2:59076/1‑139 (MQ=255)

                                         caccGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGa                                                                                 <  2:643632/139‑1 (MQ=255)

                                         caccGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGa                                                                                 <  2:301622/139‑1 (MQ=255)

                                          accGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTa                                                                                             <  2:182454/126‑1 (MQ=255)

                                          accGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTa                                                                                             >  1:182454/1‑126 (MQ=255)

                                              ggTGGATCATCGGCGTAGCTATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTAGCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGtt                                                                            <  1:1015606/139‑1 (MQ=255)

                                                  gATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGc                                                                        >  2:127822/1‑139 (MQ=255)

                                                         ggCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGgcgc                                                                 <  1:59076/139‑1 (MQ=255)

                                                         ggCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGgcgc                                                                 <  1:922841/139‑1 (MQ=255)

                                                          gCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGcgcg                                                                >  2:563515/1‑139 (MQ=255)

                                                            tCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGa                                                              <  1:127822/139‑1 (MQ=255)

                                                             cAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGAt                                                             >  1:604235/1‑139 (MQ=255)

                                                                cAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAAt                                                          >  2:521169/1‑139 (MQ=255)

                                                                    tACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTc                                                      <  1:970669/139‑1 (MQ=255)

                                                                                 ccAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCa                                         >  2:865926/1‑139 (MQ=255)

                                                                                           ggAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGa                                                                                 <  1:276521/89‑1 (MQ=255)

                                                                                           ggAAAATATTCAGCACATCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGa                                                                                 >  2:276521/1‑89 (MQ=255)

                                                                                                          caTCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGt                <  2:895388/139‑1 (MQ=255)

                                                                                                           aTCAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTg               >  2:1000730/1‑139 (MQ=255)

                                                                                                             cAGCGGTATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGac             >  2:841294/1‑139 (MQ=255)

                                                                                                                   tATCTTCTGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTgc       <  2:797686/139‑1 (MQ=255)

                                                                                                                        tctGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGccc                                          <  2:752096/99‑1 (MQ=255)

                                                                                                                        tctGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGccc                                          >  1:752096/1‑99 (MQ=255)

                                                                                                                        tctGAGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCaa  <  1:521169/139‑1 (MQ=255)

AACATAAATACGTAGGCTGGCGAAGAACCGCTCACACCGACCACCGGGTGGATCATCGGCTCAGCAATTACTTCCGCTTCGCCAAAGCAGCGGAAAATATTCAGCACATCAGCGGTATCTTCTGGGGTTACCAGCGCGTTTGGCGTTACGGAGGTCATCCCGGCATTAACCAGTGCGGGAGTGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAA  >  NC_000913/405100‑405358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A