Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I1 R1	776	60.0	3074904	90.1%	2770488	104.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	469,429	A→G	K187E (AAG→GAG)	mdlA →	putative multidrug ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	469,429	0	A	G	100.0%	212.1 / NA	59	K187E (AAG→GAG)	mdlA	putative multidrug ABC transporter ATPase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (31/28); total (31/28)

TGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCA  >  NC_000913/469295‑469564

tGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCt                                                                                                                                     >  2:1111647/1‑139 (MQ=255)

tGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCt                                                                                                                                     >  2:1524184/1‑139 (MQ=255)

            gcgcTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGc                                                                                                                                  <  2:537814/130‑1 (MQ=255)

            gcgcTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGc                                                                                                                                  >  1:537814/1‑130 (MQ=255)

            gcgcTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAggcggc                                                                                                                         >  1:193551/1‑139 (MQ=255)

            gcgcTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAggcggc                                                                                                                         >  2:1149292/1‑139 (MQ=255)

                  tgtTGATTATGATGTCTACGCAAATTAGCTGGTAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTc                                                                                                                   >  2:247643/1‑139 (MQ=255)

                  tgtTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGgcggcg                                                                                                                        <  1:608781/134‑1 (MQ=255)

                  tgtTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGgcggcg                                                                                                                        >  2:608781/1‑134 (MQ=255)

                    ttGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCa                                                                                                                 >  1:1266398/1‑139 (MQ=255)

                            gatgTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAg                                                                                                                                       >  2:358702/1‑109 (MQ=255)

                            gatgTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAg                                                                                                                                       <  1:358702/109‑1 (MQ=255)

                            gatgTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGtttt                                                                                                                    >  2:78368/1‑128 (MQ=255)

                            gatgTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGtttt                                                                                                                    <  1:78368/128‑1 (MQ=255)

                               gTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGAc                                                                                                      <  1:545543/139‑1 (MQ=255)

                                tCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGAc                                                                                                      >  2:1104589/1‑138 (MQ=255)

                                tCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGAc                                                                                                      <  1:1104589/138‑1 (MQ=255)

                                 cTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCg                                                                                                    >  2:264815/1‑139 (MQ=255)

                                          ttAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGa                                                                                           >  1:39461/1‑139 (MQ=255)

                                                ggCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCt                                                                                     <  2:1266398/139‑1 (MQ=255)

                                                  cAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCa                                                                                   <  1:264815/139‑1 (MQ=255)

                                                   aGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCAc                                                                                  <  1:1524184/139‑1 (MQ=255)

                                                              ttttCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCa                                                                       <  2:39461/139‑1 (MQ=255)

                                                              ttttCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCa                                                                       <  2:1361380/139‑1 (MQ=255)

                                                              ttttCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCa                                                                       <  2:1489585/139‑1 (MQ=255)

                                                              ttttCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCa                                                                       <  1:1111647/139‑1 (MQ=255)

                                                              ttttCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCa                                                                       <  2:193551/139‑1 (MQ=255)

                                                                  cccTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAg                                                                                                                                       >  1:933282/1‑71 (MQ=255)

                                                                   ccTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATc                                                                  >  2:843049/1‑139 (MQ=255)

                                                                   ccTGTTGCCGATGCCAGTGATGACGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATc                                                                  >  1:716417/1‑139 (MQ=255)

                                                                       ttGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAg                                                              >  1:643946/1‑139 (MQ=255)

                                                                       ttGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAg                                                              >  1:333457/1‑139 (MQ=255)

                                                                          ccGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCt                                                           <  1:247643/139‑1 (MQ=255)

                                                                           cGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCtt                                                          <  2:547378/139‑1 (MQ=255)

                                                                           cGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCtt                                                          <  2:643946/139‑1 (MQ=255)

                                                                                 cAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCt                                                    <  1:1149292/139‑1 (MQ=255)

                                                                                         gCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATc                                            >  1:1122495/1‑139 (MQ=255)

                                                                                            aTCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGcc                                         <  2:333457/139‑1 (MQ=255)

                                                                                            aTCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGcc                                         >  2:505437/1‑139 (MQ=255)

                                                                                                tGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTc                                                                                                                   <  2:1405739/61‑1 (MQ=255)

                                                                                                tGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTc                                                                                                                   >  1:1405739/1‑61 (MQ=255)

                                                                                                   ttAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCg                                                                                                    <  1:985507/73‑1 (MQ=255)

                                                                                                   ttAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCg                                                                                                    >  2:985507/1‑73 (MQ=255)

                                                                                                      aGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGt                                      >  2:1075877/1‑132 (MQ=255)

                                                                                                      aGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGt                                      <  1:1075877/132‑1 (MQ=255)

                                                                                                        cgcAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTAt                             >  1:615138/1‑139 (MQ=255)

                                                                                                           aaCGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTg                          >  1:1513322/1‑139 (MQ=255)

                                                                                                                  aTGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCtt                                                                                                            <  2:1025185/50‑1 (MQ=255)

                                                                                                                  aTGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCtt                                                                                                            >  1:1025185/1‑50 (MQ=255)

                                                                                                                     cTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCt                                                                                     >  1:864442/1‑70 (MQ=255)

                                                                                                                     cTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCt                                                                                     <  2:864442/70‑1 (MQ=255)

                                                                                                                        tGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCa                                                                                                                 <  2:1472633/39‑1 (MQ=255)

                                                                                                                        tGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCa                                                                                                                 >  1:1472633/1‑39 (MQ=255)

                                                                                                                               aCGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATAcc      >  1:578932/1‑139 (MQ=255)

                                                                                                                                  cTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCg                                 >  2:440249/1‑109 (MQ=255)

                                                                                                                                  cTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCg                                 <  1:440249/109‑1 (MQ=255)

                                                                                                                                  cTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCgg                    <  2:476825/122‑1 (MQ=255)

                                                                                                                                  cTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCgg                    >  1:476825/1‑122 (MQ=255)

                                                                                                                                   tttGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCa  <  2:1122495/139‑1 (MQ=255)

TGGTGATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATGCCGAAGATACCGGCA  >  NC_000913/469295‑469564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A