Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I1 R1	781	46.9	2231650	93.5%	2086592	109.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	469,429	A→G	K187E (AAG→GAG)	mdlA →	putative multidrug ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	469,429	0	A	G	100.0%	144.2 / NA	41	K187E (AAG→GAG)	mdlA	putative multidrug ABC transporter ATPase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/19); total (22/19)

GATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATG  >  NC_000913/469299‑469549

gATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGca                                                                                                                  >  1:1000176/1‑139 (MQ=255)

      cTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAggcg                                                                                                            >  1:67104/1‑139 (MQ=255)

         cgcTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACCGgcggcg                                                                                                         >  1:146465/1‑139 (MQ=255)

         cgcTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGgcggcg                                                                                                         <  1:321348/139‑1 (MQ=255)

                        gatgTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAAt                                                                                          >  1:1038972/1‑139 (MQ=255)

                        gatgTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAAt                                                                                          >  1:305223/1‑139 (MQ=255)

                             cTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCg                                                                                     >  2:164491/1‑139 (MQ=255)

                              tACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGTCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGc                                                                                    >  2:702954/1‑139 (MQ=255)

                              tACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCTCAGGCGGCGTTTTCCAGTCTTAATGACCGc                                                                                    >  1:691905/1‑139 (MQ=255)

                              tACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGc                                                                                    >  2:939137/1‑139 (MQ=255)

                              tACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGc                                                                                    >  1:598957/1‑139 (MQ=255)

                                cGCAAATTAGCTGGCAGTTGACCTTATTCTCCCTATTGCCGATGCCAGTCATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCAc                                                                                  >  2:720296/1‑139 (MQ=255)

                                  cAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCAccc                                                                                <  2:67104/139‑1 (MQ=255)

                                           tGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGcc                                                                       <  1:614185/139‑1 (MQ=255)

                                            ggCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCt                                                                      <  2:146465/139‑1 (MQ=255)

                                               aGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCAc                                                                   <  2:598957/139‑1 (MQ=255)

                                                    aCCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCTCAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTa                                                              <  2:691905/139‑1 (MQ=255)

                                                          ttttCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCa                                                        <  2:56192/139‑1 (MQ=255)

                                                          ttttCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCa                                                        <  2:1038972/139‑1 (MQ=255)

                                                          ttttCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCa                                                        <  1:164491/139‑1 (MQ=255)

                                                               ccTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATc                                                   <  2:1000176/139‑1 (MQ=255)

                                                                   ttGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCGTTGAGCTGGCCCAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCCTGATCAAAg                                               >  2:544021/1‑139 (MQ=255)

                                                                                tGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGa                                  >  1:101882/1‑139 (MQ=255)

                                                                                               ttAAGCGCAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGc                   >  2:360999/1‑139 (MQ=255)

                                                                                                    cgcAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGcc                                                                       >  2:520597/1‑82 (MQ=255)

                                                                                                    cgcAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGcc                                                                       <  1:520597/82‑1 (MQ=255)

                                                                                                    cgcAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTAt              >  1:763968/1‑139 (MQ=255)

                                                                                                    cgcAACGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTAt              >  2:502046/1‑139 (MQ=255)

                                                                                                       aaCGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAggcggc                                                                                                          >  2:205142/1‑44 (MQ=255)

                                                                                                       aaCGGCGATGCTTTGCATGAACGCTTTGAGCTGGCACAggcggc                                                                                                          <  1:205142/44‑1 (MQ=255)

                                                                                                             gATGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCgg     <  1:939137/139‑1 (MQ=255)

                                                                                                              aTGCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGa    <  1:502046/139‑1 (MQ=255)

                                                                                                                gCTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATg  <  2:305223/139‑1 (MQ=255)

                                                                                                                 cTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCAc                                                                   >  2:1034484/1‑73 (MQ=255)

                                                                                                                 cTTTGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCAc                                                                   <  1:1034484/73‑1 (MQ=255)

                                                                                                                    tGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCcgcg      >  2:1057006/1‑131 (MQ=255)

                                                                                                                    tGCATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCcgcg      <  1:1057006/131‑1 (MQ=255)

                                                                                                                      cATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCt                                     >  2:1044784/1‑98 (MQ=255)

                                                                                                                      cATGAACGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCt                                     <  1:1044784/98‑1 (MQ=255)

                                                                                                                           aCGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCAc                                                                                  >  1:730388/1‑48 (MQ=255)

                                                                                                                           aCGCTTTGAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCAc                                                                                  <  2:730388/48‑1 (MQ=255)

GATGGGCTGCGCTGTGTTGATTATGATGTCTACGCAAATTAGCTGGCAGTTGACCTTATTTTCCCTGTTGCCGATGCCAGTGATGGCGATCATGATTAAGCGCAACGGCGATGCTTTGCATGAACGCTTTAAGCTGGCACAGGCGGCGTTTTCCAGTCTTAATGACCGCACCCAGGAAAGCCTCACCAGTATCCGCATGATCAAAGCCTTTGGTCTGGAAGATCGCCAGTCGGCGTTATTTGCCGCGGATG  >  NC_000913/469299‑469549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A