Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F1 I1 R1
|
776 |
60.0 |
3074904 |
90.1% |
2770488 |
104.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
835,596 |
Δ1 bp |
coding (349/963 nt) |
ybiB → |
putative family 3 glycosyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 835,593 | 0 | G | . | 100.0%
| 101.2
/ NA
| 25 | coding (346/963 nt) | ybiB | putative family 3 glycosyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (11/14); total (11/14) |
ATCAAGCTGACGCCGCCAGCAGGCAAGCCGATGCCGATTGTCATCCCCAGTTACAACGGCGCGCGTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCACGGGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGGCAGGCGCAGGCGAAGCTCGACGAACATCAACCG > NC_000913/835458‑835706
|
aTCAAGCTGACGCCGCCAGCAGGCAAGCCGATGCCGATTGTCATCCCCAGTTACAACGGCGCGCGTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCACgggt < 1:480773/139‑2 (MQ=255)
gCTGACGCCGCCAGCAGGCAAGCCGATGCCGATTGTCATCCCCAGTTACAACGGCGCGCGTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGCTTTCCTGAGGTGGTTCAC‑GGGTTAGCg > 2:948802/1‑139 (MQ=255)
tGACGCCGCCAGCAGGCAAGCCGATGCCGATTGTCATCCCCAGTTACAACGGCGCGCGTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGaa > 2:789948/1‑139 (MQ=255)
gCAAGCCGATGCCGATTGTCATCCCCAGTTACAGCGGCGCGCGTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTg < 2:522413/139‑1 (MQ=255)
aTCCCCAGTTACAACGGCGCGCGTAAACAGGCCAACCTGACGCCGTTGTTGGCGTTCCTCCTGCACAAACTCGGTTCTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGa > 2:322969/1‑139 (MQ=255)
tCCCCAGTTACAACGGCGCGCGTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGaa > 1:47434/1‑139 (MQ=255)
tCCCCAGTTACAACGGCGCGCGTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGaa < 1:789948/139‑1 (MQ=255)
tCCCCAGTTACAACGGCGCGCGTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGaa < 2:395820/139‑1 (MQ=255)
gTTACTACGGCGCGCGTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTGCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATg < 1:952403/139‑1 (MQ=255)
aaCGGCGCGCGTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTAt > 1:703123/1‑139 (MQ=255)
cGGCGCGCGTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCACg < 2:973225/80‑1 (MQ=255)
cGGCGCGCGTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCACg > 1:973225/1‑80 (MQ=255)
gcgcgTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAAcc < 2:81074/113‑1 (MQ=255)
gcgcgTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAAcc > 1:81074/1‑113 (MQ=255)
gcgcgTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGcc > 1:623991/1‑139 (MQ=255)
cgcgTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAACCCATTTTTGAATTGATGGGTATTACGCCa > 1:1074895/1‑139 (MQ=255)
cgcgTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCa > 2:117936/1‑139 (MQ=255)
aGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCAc < 1:322969/139‑1 (MQ=255)
cAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCACgggt > 2:810431/1‑64 (MQ=255)
cAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCACgggt < 1:810431/65‑2 (MQ=255)
ccTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGgc < 1:986075/139‑1 (MQ=255)
cGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGgcaggcg < 2:147709/139‑1 (MQ=255)
cGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGgcaggcg > 2:817073/1‑139 (MQ=255)
gttgttGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGgcaggcgcagg < 1:817073/139‑1 (MQ=255)
ttgttgGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCACgggt > 1:978427/1‑51 (MQ=255)
ttgttgGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCACgggt < 2:978427/52‑2 (MQ=255)
aTTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGGCAGGCGCAGGCGAAGCTcga < 2:623991/139‑1 (MQ=255)
ccTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGGCAGGCGCAGGCGAAGCTCGACGAAc < 2:1074895/139‑1 (MQ=255)
aaCTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGGCAGGCGCAGGCGAAGCTCGACGAACATCAACCg < 2:703123/139‑1 (MQ=255)
aaCTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGGCAGGCGCAGGCGAAGCTCGACGAACATCAACCg < 1:117936/139‑1 (MQ=255)
aCTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGtt < 2:483249/95‑1 (MQ=255)
aCTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGtt > 1:483249/1‑95 (MQ=255)
|
ATCAAGCTGACGCCGCCAGCAGGCAAGCCGATGCCGATTGTCATCCCCAGTTACAACGGCGCGCGTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCACGGGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGGCAGGCGCAGGCGAAGCTCGACGAACATCAACCG > NC_000913/835458‑835706
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
