Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F1 I2 R1
|
766 |
64.2 |
3232166 |
90.9% |
2938038 |
104.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
835,596 |
Δ1 bp |
coding (349/963 nt) |
ybiB → |
putative family 3 glycosyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 835,593 | 0 | G | . | 97.5%
| 156.7
/ ‑2.5
| 40 | coding (346/963 nt) | ybiB | putative family 3 glycosyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base . (20/19); minor base C (1/0); total (21/19) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCTGACGCCGCCAGCAGGCAAGCCGATGCCGATTGTCATCCCCAGTTACAACGGCGCGCGTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCACGGGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGGCAGGCGCAGGCGAAGCTCGACGAACATCAACCGGTGTTTATGCCAGTCGGCGC > NC_000913/835463‑835726
|
gCTGACGCCGCCAGCAGGCAAGCCGATGCCGATTGTCATCCCCAGTTACAACGGCGCGCGTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCg < 1:544535/139‑1 (MQ=255)
cagcagGCAAGCCGATGCCGATTGTCATCCCCAGTTACAACGGCGCGCGTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAAcc < 2:29200/139‑1 (MQ=255)
cAAGCCGATGCCGATTGTCATCCCCAGTTACAACGGCGCGCGTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGc < 2:448869/139‑1 (MQ=255)
atgccgatTGTCATCCCCAGTTACAACGGCGCGCGTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGctgactga > 1:1437782/1‑139 (MQ=255)
aTCCCCAGTTACAACGGCGCGCGTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTC‑CCGGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGa > 2:218057/1‑139 (MQ=255)
cccAGTTACAACGGCGCGCGTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCAAAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATATTTGAAtt > 2:958125/1‑139 (MQ=255)
aGTTACAACGGCGCGCGTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGAt < 1:218057/139‑1 (MQ=255)
gTTACAACGGCGCGCGTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATg > 2:588315/1‑139 (MQ=255)
ttACAACGGCGCGCGTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATgg > 1:714351/1‑139 (MQ=255)
gcgcgTAAATACGCCAACCTGACCCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGcc > 1:1298185/1‑139 (MQ=255)
gcgcgTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAAc > 1:751642/1‑112 (MQ=255)
gcgcgTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAAc < 2:751642/112‑1 (MQ=255)
gcgcgTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGcc > 2:1383292/1‑139 (MQ=255)
cgcgTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATg > 1:1598229/1‑127 (MQ=255)
cgcgTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATg < 2:1598229/127‑1 (MQ=255)
cgcgTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCa > 2:463281/1‑139 (MQ=255)
aaCAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTg < 1:588315/139‑1 (MQ=255)
gCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTg > 1:1270086/1‑89 (MQ=255)
gCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTg < 2:1270086/89‑1 (MQ=255)
gCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGct < 1:516707/91‑1 (MQ=255)
gCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGct > 2:516707/1‑91 (MQ=255)
gCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCAcgg < 2:1356526/139‑1 (MQ=255)
ccAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCACgggtt > 2:761236/1‑65 (MQ=255)
ccAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCACgggtt < 1:761236/67‑3 (MQ=255)
ccAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCAcggc > 2:1121442/1‑139 (MQ=255)
aaCCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCAcggcgg < 1:1121442/139‑1 (MQ=255)
ttgttgGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGgcaggcgcaggc > 2:326711/1‑139 (MQ=255)
ttCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGGCAGGCGCAGGCGAAGCTcgac > 2:76093/1‑139 (MQ=255)
tctcCTGCACAAACTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGGCAGGCGCAGGCGAAGCTcgacg < 1:1383292/139‑1 (MQ=255)
aaCTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGGCAGGCGCAGGCGAAGCTCGACGAACATCAACCg < 1:958125/139‑1 (MQ=255)
aaCTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGGCAGGCGCAGGCGAAGCTCGACGAACATCAACCg < 1:326711/139‑1 (MQ=255)
aCTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGaa > 1:844773/1‑72 (MQ=255)
aCTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGaa < 2:844773/72‑1 (MQ=255)
aCTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGgcaggcg > 1:78832/1‑111 (MQ=255)
aCTCGGTTTTCCTGTGGTGGTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGgcaggcg < 2:78832/111‑1 (MQ=255)
tggtggTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTAt > 2:1237220/1‑69 (MQ=255)
tggtggTTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTAt < 1:1237220/69‑1 (MQ=255)
gtTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGGCAGGCGCAGGCGAAGCTCGACGAACATCAACCg < 2:1480466/119‑1 (MQ=255)
gtTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGGCAGGCGCAGGCGAAGCTCGACGAACATCAACCg > 1:1480466/1‑119 (MQ=255)
gtTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGGCAGGCGCAGGCGAAGCTCGACGAACATCAACCGGTGTTTATGCCAGTCgg < 2:1432012/136‑1 (MQ=255)
gtTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGGCAGGCGCAGGCGAAGCTCGACGAACATCAACCGGTGTTTATGCCAGTCgg > 1:1432012/1‑136 (MQ=255)
gtTCAC‑GGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGGCAGGCGCAGGCGAAGCTCGACGAACATCAACCGGTGTTTATGCCAGTCGgcgc > 1:757381/1‑139 (MQ=255)
|
GCTGACGCCGCCAGCAGGCAAGCCGATGCCGATTGTCATCCCCAGTTACAACGGCGCGCGTAAACAGGCCAACCTGACGCCGTTGTTGGCGATTCTCCTGCACAAACTCGGTTTTCCTGTGGTGGTTCACGGGGTTAGCGAAGATCCAACCCGCGTGCTGACTGAAACCATTTTTGAATTGATGGGTATTACGCCAACGTTGCACGGCGGGCAGGCGCAGGCGAAGCTCGACGAACATCAACCGGTGTTTATGCCAGTCGGCGC > NC_000913/835463‑835726
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
