Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F1 I1 R1
|
776 |
60.0 |
3074904 |
90.1% |
2770488 |
104.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,557,112 |
C→T |
*309* (TAG→TAA) |
ddpF ← |
D,D‑dipeptide ABC transporter ATPase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,557,112 | 0 | C | T | 100.0%
| 134.7
/ NA
| 43 | *309* (TAG→TAA) | ddpF | D,D‑dipeptide ABC transporter ATPase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (23/20); total (23/20) |
ATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTCTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGT > NC_000913/1556976‑1557234
|
aTCCAGAAAGCAAAAGCAGGATGTCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTa < 2:1033678/139‑1 (MQ=255)
ggATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCg < 2:19429/139‑1 (MQ=255)
ggATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCg > 1:1454169/1‑139 (MQ=255)
ggATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCg < 1:481745/139‑1 (MQ=255)
ctctcAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAg < 2:175421/131‑1 (MQ=255)
ctctcAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAg > 1:175421/1‑131 (MQ=255)
cAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTGGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGAGAGGGAATGCCTGATGCGTTGTTAGCATCGTTACAGCGCCCGCCAGCACCGGAGATCAcgtccgtc < 2:1205565/139‑1 (MQ=255)
aCTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCAcgtccgtc < 1:1254461/134‑1 (MQ=255)
aCTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCAcgtccgtc > 2:1254461/1‑134 (MQ=255)
aCTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTccc > 2:915941/1‑139 (MQ=255)
tGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCtt > 1:1285025/1‑139 (MQ=255)
aaaGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACgtccgtcc < 2:363777/131‑1 (MQ=255)
aaaGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACgtccgtcc > 1:363777/1‑131 (MQ=255)
cGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCt > 2:705256/1‑139 (MQ=255)
aaaTTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAAt < 2:1454169/139‑1 (MQ=255)
cTGGATTACCAGTTGAAATCGTAAAGCATTGCCGAATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTTTTAGCATCTTTACAGTGCCCGCCAGCACCGGAGCTCACGCCCGTCCTCCCTTATTGCTAATGATTGCCg < 2:786067/139‑1 (MQ=255)
tACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAgcg < 1:1327832/81‑1 (MQ=255)
tACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAgcg > 2:1327832/1‑81 (MQ=255)
aCCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGCATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTca < 1:915941/139‑1 (MQ=255)
ccAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCAcgtccgtc > 1:435206/1‑107 (MQ=255)
ccAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCAcgtccgtc < 2:435206/107‑1 (MQ=255)
tGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGCTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCGCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCg > 2:698041/1‑139 (MQ=255)
aaaGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGcc > 2:228607/1‑139 (MQ=255)
gCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCa > 2:954337/1‑139 (MQ=255)
cgcgTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCt > 1:742731/1‑66 (MQ=255)
cgcgTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCt < 2:742731/66‑1 (MQ=255)
cgcgTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTa > 1:1321339/1‑139 (MQ=255)
cgTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGcc > 1:133713/1‑111 (MQ=255)
cgTGAATGCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGcc < 2:133713/111‑1 (MQ=255)
gCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCa < 1:1212614/112‑1 (MQ=255)
gCCTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCa > 2:1212614/1‑112 (MQ=255)
cTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCAcgtccgtc < 2:50694/56‑1 (MQ=255)
cTGATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCAcgtccgtc > 1:50694/1‑56 (MQ=255)
gATGCGTTGTTAGCATCTTTACAGCGCCCGCTAGCACCGGAGCTCAcgtccg > 2:1300226/1‑52 (MQ=255)
gATGCGTTGTTAGCATCTTTACAGCGCCCGCTAGCACCGGAGCTCAcgtccg < 1:1300226/52‑1 (MQ=255)
gATGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAAc > 2:11633/1‑139 (MQ=255)
tGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCgg < 2:1056167/103‑1 (MQ=255)
tGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCgg > 1:1056167/1‑103 (MQ=255)
tGCGTTGTTAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGt > 1:785038/1‑139 (MQ=255)
gttAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTc > 2:157047/1‑76 (MQ=255)
gttAGCATCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTc < 1:157047/76‑1 (MQ=255)
aTCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTca > 2:133059/1‑71 (MQ=255)
aTCTTTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTca < 1:133059/71‑1 (MQ=255)
|
ATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAGGTACTGAAAGCGGAAATTACGCTGGATTACCAGTTGAAATCGTAAAGCATTGCCGGATGACGCGTCAGGCGCGTGAATGCCTGATGCGTTGTTAGCATCTCTACAGCGCCCGCCAGCACCGGAGCTCACGTCCGTCCTCCCTTATTGCTAATGATTGCCGGACTTCACATCCGTGGGTTGCCAGCGGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGT > NC_000913/1556976‑1557234
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
