Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I1 R1	776	60.0	3074904	90.1%	2770488	104.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,610,107	C→T	A192T (GCA→ACA)	uxaB ←	altronate oxidoreductase, NAD‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,610,107	0	C	T	100.0%	127.4 / NA	40	A192T (GCA→ACA)	uxaB	altronate oxidoreductase, NAD‑dependent
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (17/23); total (17/23)

AACCATCGTGATAACCCAGTTCTTCTTCCAGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCA  >  NC_000913/1609972‑1610235

aaCCATCGTGATAACCCAGTTCTTCTTCCAGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGTCCa                                                                                                                               <  2:1410622/139‑1 (MQ=255)

aaCCATCGTAATAACCCAGTTCTTCTTCCAGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGTCCa                                                                                                                               <  1:734321/139‑1 (MQ=255)

 aCCATCGTGCTAACCCAGTTCTTCTTCCAGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGTCCAc                                                                                                                              >  1:1028757/1‑139 (MQ=255)

  ccATCGTGATAACCCAGTTCTTCTTCCAGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGTCCAct                                                                                                                             <  2:524533/139‑1 (MQ=255)

   cATCGTGATAACCCAGTTCTTCTTCCAGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGTCCActc                                                                                                                            >  2:60876/1‑139 (MQ=255)

           tAACCCAGTTCTTCTTCCAGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGTCCACTCTTGCGCAt                                                                                                                    <  1:244992/139‑1 (MQ=255)

                              gTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGTCCACTCTTGCGCATATCGCAGCACCAGTTCACg                                                                                                 <  2:878110/139‑1 (MQ=255)

                                        ttCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGTCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCg                                                                                       <  1:15665/139‑1 (MQ=255)

                                        ttCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGTCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCg                                                                                       >  1:805940/1‑139 (MQ=255)

                                         tCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGTCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGc                                                                                      >  2:1161305/1‑139 (MQ=255)

                                           aTCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGTCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCa                                                                                    <  1:60876/139‑1 (MQ=255)

                                                     tAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGTCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGtcaa                                                                          <  1:1161305/139‑1 (MQ=255)

                                                              aCGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGTCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTcac                                                                 >  1:280398/1‑139 (MQ=255)

                                                               cGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTCGCAGTGTCCACTCTTGCGCATAGCGCAGCACCAGTACACGCAACGCGTCGCCATTGTAGACATTCAACTcaca                                                                <  2:1028757/139‑1 (MQ=255)

                                                               cGATACCGTCATCCAGCGTCGAACAGAAGCTGTTAGCATGATACAGCCATTCGATCAATGCTTCTATCAGTGTCCACACTTGCGTATAGCAGAGCACCAGTTCGCGCAACGCGACGCCAGTGTAGTCAATCACCTcaca                                                                <  2:1058189/139‑1 (MQ=255)

                                                                  tACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGTCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTcaca                                                                >  2:1523633/1‑136 (MQ=255)

                                                                  tACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGTCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTcaca                                                                <  1:1523633/136‑1 (MQ=255)

                                                                   aCGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGTCCACTCTTGCGCATAGCGCAGCACCAGTTCACGc                                                                                                <  1:115270/103‑1 (MQ=255)

                                                                   aCGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGTCCACTCTTGCGCATAGCGCAGCACCAGTTCACGc                                                                                                >  2:115270/1‑103 (MQ=255)

                                                                     ggTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGTCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAat                                                          <  2:280398/139‑1 (MQ=255)

                                                                             aGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGTCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATcc                                                  <  1:846348/139‑1 (MQ=255)

                                                                                 tAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGTCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAAcc                                              >  1:8251/1‑139 (MQ=255)

                                                                                   gAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGTCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATcc                                                  <  1:1239339/133‑1 (MQ=255)

                                                                                   gAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGTCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATcc                                                  >  2:1239339/1‑133 (MQ=255)

                                                                                    aaCAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGTCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCa                                                                                    <  2:782872/98‑1 (MQ=255)

                                                                                    aaCAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGTCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCa                                                                                    >  1:782872/1‑98 (MQ=255)

                                                                                         aaGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGTCCa                                                                                                                               <  1:578548/50‑1 (MQ=255)

                                                                                         aaGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGTCCa                                                                                                                               >  2:578548/1‑50 (MQ=255)

                                                                                          aGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGTCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAAc                                                                     >  2:669118/1‑107 (MQ=255)

                                                                                          aGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGTCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAAc                                                                     <  1:669118/107‑1 (MQ=255)

                                                                                              gTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGTCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTca                                                                  <  2:1450690/106‑1 (MQ=255)

                                                                                              gTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGTCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTca                                                                  >  1:1450690/1‑106 (MQ=255)

                                                                                              gTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGTCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTAAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCa                                 >  2:1186922/1‑139 (MQ=255)

                                                                                                tAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGTCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCAcc                               <  2:8251/139‑1 (MQ=255)

                                                                                                         ccAGCCATTGAATAAATGCTTCTGGCAGTGTCCACTCTTGCGCATAg                                                                                                                  <  1:79948/47‑1 (MQ=255)

                                                                                                         ccAGCCATTGAATAAATGCTTCTGGCAGTGTCCACTCTTGCGCATAg                                                                                                                  >  2:79948/1‑47 (MQ=255)

                                                                                                         ccAGCCATTGAATAAATGCTTCTGGCAGTGTCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATcc                                                  <  2:519624/111‑1 (MQ=255)

                                                                                                         ccAGCCATTGAATAAATGCTTCTGGCAGTGTCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATcc                                                  >  1:519624/1‑111 (MQ=255)

                                                                                                                       aaTGCTTCTGGCAGTGTCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGa        >  1:1069950/1‑139 (MQ=255)

                                                                                                                             tCTGGCAGTGTCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAAcagca  <  2:805940/139‑1 (MQ=255)

AACCATCGTGATAACCCAGTTCTTCTTCCAGTTTAGCCACTTCATCGCGCGGATAACCGGTAACGATACGGTCCACCAGCGTAGAACAGAAGCTGTTAGCCTGATCCAGCCATTGAATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCA  >  NC_000913/1609972‑1610235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A