Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I1 R1	776	60.0	3074904	90.1%	2770488	104.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,794,172	C→T	327 (TAG→TAA)	btuC ←	vitamin B12 ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,794,172	0	C	T	100.0%	148.3 / NA	46	327 (TAG→TAA)	btuC	vitamin B12 ABC transporter permease
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (23/23); total (23/23)

GGCGAACTTCTCCAGCGTGGTCACTTCACCGTCGATATCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGCTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCC  >  NC_000913/1794038‑1794306

ggCGAACTTCTCCAGCGTGGTCACTTCACCGTCGATATCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAAc                                                                                                                                    <  1:1009160/139‑1 (MQ=255)

   gAACTTCTCCAGCGTGGTCACTTCACCGTCGATATCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTc                                                                                                                                 <  1:641094/139‑1 (MQ=255)

               cGTGGTCACTTCACCGTCGATATCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACa                                                                                                                     >  1:695239/1‑139 (MQ=255)

                                   tatCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGgc                                                                                                 <  1:98496/139‑1 (MQ=255)

                                             tACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCt                                                                                                                               <  1:1017862/99‑1 (MQ=255)

                                             tACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCt                                                                                                                               >  2:1017862/1‑99 (MQ=255)

                                             tACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAAc                                                                                                                <  2:320751/114‑1 (MQ=255)

                                             tACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAAc                                                                                                                >  1:320751/1‑114 (MQ=255)

                                               cGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAaca                                                                                                      <  2:569000/122‑1 (MQ=255)

                                               cGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAaca                                                                                                      >  1:569000/1‑122 (MQ=255)

                                               cGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGtt                                                                                     >  1:396250/1‑139 (MQ=255)

                                                  tcgtcAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCg                                                                                  <  1:127269/139‑1 (MQ=255)

                                                         aaTGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCa                                                                           >  2:635530/1‑139 (MQ=255)

                                                         aaTGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCa                                                                           <  1:1323043/139‑1 (MQ=255)

                                                                   tGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAgg                                                                 >  1:1350529/1‑139 (MQ=255)

                                                                     cATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGc                                                                        <  2:430765/130‑1 (MQ=255)

                                                                     cATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGc                                                                        <  2:430766/130‑1 (MQ=255)

                                                                     cATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGc                                                                        >  1:430766/1‑130 (MQ=255)

                                                                     cATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGc                                                                        >  1:430765/1‑130 (MQ=255)

                                                                          tttCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGctct                                                          >  2:1371538/1‑139 (MQ=255)

                                                                                        ggTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGgc                                                                                                 <  1:640704/86‑1 (MQ=255)

                                                                                        ggTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGgc                                                                                                 >  2:640704/1‑86 (MQ=255)

                                                                                         gTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTa                                                                                                                                      <  2:314808/48‑1 (MQ=255)

                                                                                            aGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAgg                                        >  2:955429/1‑139 (MQ=255)

                                                                                              tAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGgcg                                      <  2:695239/139‑1 (MQ=255)

                                                                                              tAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGgcg                                      >  2:480846/1‑139 (MQ=255)

                                                                                              tAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGgcg                                      <  2:775612/139‑1 (MQ=255)

                                                                                              tAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGgcg                                      <  1:480846/139‑1 (MQ=255)

                                                                                              tAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGgcg                                      <  2:841087/139‑1 (MQ=255)

                                                                                                   aTCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTa                                 <  2:1350529/139‑1 (MQ=255)

                                                                                                   aTCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTa                                 <  2:396250/139‑1 (MQ=255)

                                                                                                              tAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGcc                      >  2:1352345/1‑139 (MQ=255)

                                                                                                              tAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGcc                      >  2:708368/1‑139 (MQ=255)

                                                                                                                    tAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCagcagc                >  1:859334/1‑139 (MQ=255)

                                                                                                                       aCCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAAt             >  1:666023/1‑139 (MQ=255)

                                                                                                                         cGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTa                                 <  2:1149684/117‑1 (MQ=255)

                                                                                                                         cGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTa                                 >  1:1149684/1‑117 (MQ=255)

                                                                                                                          gTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATgcg          >  1:1408597/1‑139 (MQ=255)

                                                                                                                           tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCgg                                                                                                  >  2:482477/1‑50 (MQ=255)

                                                                                                                           tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCgg                                                                                                  <  1:482477/50‑1 (MQ=255)

                                                                                                                           tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCAcc                                                                                            <  2:1536218/56‑1 (MQ=255)

                                                                                                                           tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCAcc                                                                                            >  1:1536218/1‑56 (MQ=255)

                                                                                                                           tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATgcgc         >  2:1517927/1‑139 (MQ=255)

                                                                                                                           tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATgcgc         >  1:517768/1‑139 (MQ=255)

                                                                                                                           tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATgcgc         <  2:859334/139‑1 (MQ=255)

                                                                                                                                  gCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGcccc  <  1:1371538/139‑1 (MQ=255)

GGCGAACTTCTCCAGCGTGGTCACTTCACCGTCGATATCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGCTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCC  >  NC_000913/1794038‑1794306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A