Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F1 I1 R1
|
776 |
60.0 |
3074904 |
90.1% |
2770488 |
104.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,823,899 |
G→A |
V129M (GTG→ATG) |
cho → |
endonuclease of nucleotide excision repair |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,823,899 | 0 | G | A | 100.0%
| 94.3
/ NA
| 31 | V129M (GTG→ATG) | cho | endonuclease of nucleotide excision repair |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (15/16); total (15/16) |
ATGTACCGCCGGTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGC > NC_000913/1823763‑1824018
|
atGTACCGCCGGTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATgtgatg < 2:1414691/139‑1 (MQ=255)
tGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGATGTATGCCAAAGAGGTGGATTTTTCACg < 2:363632/139‑1 (MQ=255)
tGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGATGTATGCCAAAGAGGTGGATTTTTCACGAGccccc > 2:1197269/1‑139 (MQ=255)
aGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGATGTATGCCAAagag < 1:591909/115‑1 (MQ=255)
aGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGATGTATGCCAAagag > 2:591909/1‑115 (MQ=255)
cgcgATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGATGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCaa < 2:309814/136‑1 (MQ=255)
cgcgATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGATGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCaa > 1:309814/1‑136 (MQ=255)
cgcgATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGATGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCaa < 2:752715/136‑1 (MQ=255)
cgcgATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGATGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCaa > 1:752715/1‑136 (MQ=255)
cgcgATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGATGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCt > 1:416253/1‑139 (MQ=255)
aaTCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGATGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGc < 2:416253/139‑1 (MQ=255)
acaGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGATGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAAt < 1:1197269/139‑1 (MQ=255)
ttAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGATGTATGCCAAAGAGGTGGATTTTTCACg < 1:833640/97‑1 (MQ=255)
ttAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGATGTATGCCAAAGAGGTGGATTTTTCACg > 2:833640/1‑97 (MQ=255)
ggTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGATGTATGCCAAAGAGGTGGATTTTTCACg < 2:156166/88‑1 (MQ=255)
ggTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGATGTATGCCAAAGAGGTGGATTTTTCACg > 1:156166/1‑88 (MQ=255)
cgccgcAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGATGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCaa > 1:273300/1‑92 (MQ=255)
cgccgcAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGATGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCaa < 2:273300/92‑1 (MQ=255)
tgCCCTGCAATTAAATGAAAAGCGCGTCGATGTGATGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCaa > 2:185548/1‑72 (MQ=255)
tgCCCTGCAATTAAATGAAAAGCGCGTCGATGTGATGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCaa < 1:185548/72‑1 (MQ=255)
tGCAATTAAATGAAAAGCGCGTCGATGTGATGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACaa > 1:90892/1‑134 (MQ=255)
tGCAATTAAATGAAAAGCGCGTCGATGTGATGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACaa < 2:90892/134‑1 (MQ=255)
tGCAATTAAATGAAAAGCGCGTCGATGTGATGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACt > 2:641537/1‑139 (MQ=255)
ttAAATGAAAAGCGCGTCGATGTGATGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGtt < 2:1220719/139‑1 (MQ=255)
aaaTGAAAAGCGCGTCGATGTGATGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTAt > 1:526153/1‑139 (MQ=255)
aaaTGAAAAGCGCGTCGATGTGATGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTCCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAAGAAAAACTTTGTTAt > 1:939233/1‑139 (MQ=255)
tGAAAAGCGCGTCGATGTGATGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGc < 1:641537/139‑1 (MQ=255)
tGAAAAGCGCGTCGATGTGATGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTCTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGc < 2:939233/139‑1 (MQ=255)
cgTCGATGTGATGTATGCCAAAGAGGTGGATTTTTCACg > 2:196888/1‑39 (MQ=255)
cgTCGATGTGATGTATGCCAAAGAGGTGGATTTTTCACg < 1:196888/39‑1 (MQ=255)
gTCGATGTGATGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGt < 2:60830/61‑1 (MQ=255)
gTCGATGTGATGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGt > 1:60830/1‑61 (MQ=255)
|
ATGTACCGCCGGTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGC > NC_000913/1823763‑1824018
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
