Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F1 I1 R1 776 60.0 3074904 90.1% 2770488 104.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,833,009 (G)5→4 coding (582/807 nt) xthA → exonuclease III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,833,0050G.100.0% 233.1 / NA 56coding (578/807 nt)xthAexonuclease III
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (33/23);  total (33/23)

ATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTGGGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGC  >  NC_000913/1832869‑1833136
                                                                                                                                        |                                                                                                                                   
attattGGCGATATGAATATCAGCCCAAGAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGAGCTGGCTGCGTACCGGT‑AATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATTAG‑Tggggg                                                                                                                                 >  2:443809/1‑139 (MQ=255)
aTTATGGGCGATATGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTggg                                                                                                                                   >  2:289428/1‑139 (MQ=255)
          atatGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGAt                                                                                                                        >  1:1297739/1‑139 (MQ=255)
          atatGAATATCAGCCCTACAGATCTGGATATCGGCATTGGAGAAGAAAACCGTCAGCGCTGGCTGCGTAACGGTAAATGCTCTTTCCTGCCGGCCGACCGCGAAAGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGAt                                                                                                                        >  2:1355794/1‑139 (MQ=255)
                 tatCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTcc                                                                                                                 >  1:288571/1‑139 (MQ=255)
                 tatCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTcc                                                                                                                 >  2:361050/1‑139 (MQ=255)
                     aGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGt                                                                                                                            >  1:917484/1‑124 (MQ=255)
                     aGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGt                                                                                                                            <  2:917484/124‑1 (MQ=255)
                       cccTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATg                                                                                                           >  1:734005/1‑139 (MQ=255)
                         cTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCg                                                                                                         <  1:361050/139‑1 (MQ=255)
                         cTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCg                                                                                                         >  2:443695/1‑139 (MQ=255)
                           aCAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGaa                                                                                                       >  2:220198/1‑139 (MQ=255)
                                 cTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCa                                                                                                 >  1:628886/1‑139 (MQ=255)
                                    gATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAAc                                                                                              >  1:740714/1‑139 (MQ=255)
                                    gATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAAc                                                                                              >  2:429965/1‑139 (MQ=255)
                                      tatCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAAcag                                                                                            <  2:158914/139‑1 (MQ=255)
                                        tCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAAcagca                                                                                          <  1:429965/139‑1 (MQ=255)
                                         cGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAAcagcag                                                                                         >  1:1031031/1‑139 (MQ=255)
                                         cGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAAcagcag                                                                                         >  2:570655/1‑139 (MQ=255)
                                              ttGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGt                                                                                    <  1:570655/139‑1 (MQ=255)
                                                                      ggctgCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCa                                                            >  2:1003005/1‑139 (MQ=255)
                                                                       gctgCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTtctc                                                                               <  2:15675/119‑1 (MQ=255)
                                                                       gctgCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTtctc                                                                               >  1:15675/1‑119 (MQ=255)
                                                                                 ggTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTgggg                                                                                                                                  >  2:1361063/1‑59 (MQ=255)
                                                                                 ggTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTgggg                                                                                                                                  <  1:1361063/59‑1 (MQ=255)
                                                                                    aaaTGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGa                                              <  1:443695/139‑1 (MQ=255)
                                                                                     aaTGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGAt                                                                                                                        >  2:520294/1‑64 (MQ=255)
                                                                                     aaTGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGAt                                                                                                                        <  1:520294/64‑1 (MQ=255)
                                                                                     aaTGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGAt                                             >  1:1074896/1‑139 (MQ=255)
                                                                                            tttCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATc                                                                                      <  1:9425/91‑1 (MQ=255)
                                                                                            tttCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATc                                                                                      >  2:9425/1‑91 (MQ=255)
                                                                                             ttCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCa                                                                                                 <  1:435628/79‑1 (MQ=255)
                                                                                             ttCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCa                                                                                                 >  2:435628/1‑79 (MQ=255)
                                                                                             ttCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCAt                                                                             >  2:924600/1‑99 (MQ=255)
                                                                                             ttCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCAt                                                                             <  1:924600/99‑1 (MQ=255)
                                                                                             ttCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTgg                                     >  2:1145372/1‑139 (MQ=255)
                                                                                             ttCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTgg                                     >  1:567686/1‑139 (MQ=255)
                                                                                              tCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTGGt                                    <  2:264217/139‑1 (MQ=255)
                                                                                                   ccGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTGGTCTgcg                               <  2:288571/139‑1 (MQ=255)
                                                                                                     ggAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCa                             <  1:289428/139‑1 (MQ=255)
                                                                                                      gAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCAt                            <  1:1145372/139‑1 (MQ=255)
                                                                                                      gAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCAt                            >  1:546622/1‑139 (MQ=255)
                                                                                                        agagCGCGAATGGATGGACAGCCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCATCg                          >  2:209563/1‑139 (MQ=255)
                                                                                                         gagCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCATCGa                         <  2:567686/139‑1 (MQ=255)
                                                                                                           gcgcGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCATCGAcc                       <  1:220198/139‑1 (MQ=255)
                                                                                                                    gatggaCAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTTACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCg              >  2:734664/1‑139 (MQ=255)
                                                                                                                    gatggaCAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCg              >  2:513550/1‑139 (MQ=255)
                                                                                                                      tggaCAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTAc                                                                  <  2:1302095/85‑1 (MQ=255)
                                                                                                                      tggaCAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTAc                                                                  >  1:1302095/1‑85 (MQ=255)
                                                                                                                      tggaCAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGa                                              >  1:716852/1‑105 (MQ=255)
                                                                                                                      tggaCAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGa                                              <  2:716852/105‑1 (MQ=255)
                                                                                                                      tggaCAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCgcc            >  1:631981/1‑139 (MQ=255)
                                                                                                                         aCAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCgccagc         <  2:631981/139‑1 (MQ=255)
                                                                                                                             gCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCa                                                                                                             <  2:718072/35‑1 (MQ=25)
                                                                                                                             gCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCa                                                                                                             >  1:718072/1‑35 (MQ=25)
                                                                                                                             gCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAAc     >  2:37472/1‑139 (MQ=255)
                                                                                                                              cTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTGGTc                                   >  2:78775/1‑108 (MQ=255)
                                                                                                                              cTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTGGTc                                   <  1:78775/108‑1 (MQ=255)
                                                                                                                               tgatgaGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCg   >  2:962331/1‑139 (MQ=255)
                                                                                                                                gatgaGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGc  <  1:734664/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                   
ATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTGGGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGC  >  NC_000913/1832869‑1833136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A