Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F1 I2 R1 766 64.2 3232166 90.9% 2938038 104.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,833,009 (G)5→4 coding (582/807 nt) xthA → exonuclease III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,833,0050G.100.0% 205.0 / NA 50coding (578/807 nt)xthAexonuclease III
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (28/22);  total (28/22)

GGGCGATATGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTGGGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTG  >  NC_000913/1832874‑1833138
                                                                                                                                   |                                                                                                                                     
gggCGATATGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTggggttgg                                                                                                                                >  2:173104/1‑135 (MQ=255)
     atatGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGAt                                                                                                                          >  1:706531/1‑139 (MQ=255)
                aGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGAt                                                                                                                          >  2:117401/1‑128 (MQ=255)
                aGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGAt                                                                                                                          <  1:117401/128‑1 (MQ=255)
                  cccTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTggggttgg                                                                                                                                <  2:298007/121‑5 (MQ=255)
                  cccTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTggggttgg                                                                                                                                >  1:298007/1‑117 (MQ=255)
                  cccTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTggggt                                                                                                                                   >  1:428364/1‑117 (MQ=255)
                  cccTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTggggt                                                                                                                                   <  2:428364/118‑2 (MQ=255)
                  cccTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATg                                                                                                             >  1:621552/1‑139 (MQ=255)
                  cccTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATg                                                                                                             >  2:256471/1‑139 (MQ=255)
                    cTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCg                                                                                                           >  2:600489/1‑139 (MQ=255)
                    cTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCg                                                                                                           <  1:59889/139‑1 (MQ=255)
                      aCAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGaa                                                                                                         >  1:1365827/1‑139 (MQ=255)
                      aCAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGaa                                                                                                         >  2:1123195/1‑139 (MQ=255)
                      aCAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGaa                                                                                                         >  1:466899/1‑139 (MQ=255)
                       cAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAAt                                                                                                        <  1:500662/139‑1 (MQ=255)
                                         ttGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCt                                                                                                                      >  2:1384436/1‑107 (MQ=255)
                                         ttGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCt                                                                                                                      <  1:1384436/107‑1 (MQ=255)
                                         ttGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGt                                                                                      <  2:1365827/139‑1 (MQ=255)
                                          tGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGtt                                                                                     >  2:348383/1‑139 (MQ=255)
                                             cGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTgggg                                                                                                                                    <  1:260701/90‑1 (MQ=255)
                                             cGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTgggg                                                                                                                                    >  2:260701/1‑90 (MQ=255)
                                              gaagaaAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTtctc                                                                                 <  2:328201/139‑1 (MQ=255)
                                                agaaAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCAt                                                                               <  1:256471/139‑1 (MQ=255)
                                                agaaAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCAt                                                                               <  1:348383/139‑1 (MQ=255)
                                                         tAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTgggg                                                                                                                                    <  1:354932/78‑1 (MQ=255)
                                                         tAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTgggg                                                                                                                                    >  2:354932/1‑78 (MQ=255)
                                                           aGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTAc                                                                    <  2:621552/139‑1 (MQ=255)
                                                            gcgcTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTAcc                                                                   >  2:336042/1‑139 (MQ=255)
                                                                 ggctgCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCa                                                              >  2:1061665/1‑139 (MQ=255)
                                                                  gctgCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCaa                                                             <  1:173104/139‑1 (MQ=255)
                                                                        tACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAAt                                                                                                        <  1:844977/90‑1 (MQ=255)
                                                                        tACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAAt                                                                                                        >  2:844977/1‑90 (MQ=255)
                                                                                  tGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATaa                                             >  2:1133432/1‑139 (MQ=255)
                                                                                     tctTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCTAAGCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGGTTTGACGATAAcc                                           >  2:988070/1‑138 (MQ=255)
                                                                                     tctTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAAcc                                           <  1:988070/138‑1 (MQ=255)
                                                                                     tctTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCg                                          >  1:1355110/1‑139 (MQ=255)
                                                                                      ctTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGt                                         >  1:397141/1‑139 (MQ=255)
                                                                                            tGCCGGAAGAGCGCGAATGGATGGACACGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAAcagc                                                                                             >  2:1348911/1‑81 (MQ=255)
                                                                                            tGCCGGAAGAGCGCGAATGGATGGACACGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAAcagc                                                                                             <  1:1348911/81‑1 (MQ=255)
                                                                                              ccGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTGGTCTgcg                                 >  2:1151157/1‑139 (MQ=255)
                                                                                                ggAAGAGCGCGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCa                               <  1:1123195/139‑1 (MQ=255)
                                                                                                      gcgcGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGtttt                                                     <  2:1153461/111‑1 (MQ=255)
                                                                                                      gcgcGAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGtttt                                                     >  1:1153461/1‑111 (MQ=255)
                                                                                                       cgcgAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAAcag                                                                                              >  2:1045323/1‑69 (MQ=255)
                                                                                                       cgcgAATGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAAcag                                                                                              <  1:1045323/69‑1 (MQ=255)
                                                                                                           aaTGGATGGACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCATCGACctgctg                    <  2:397141/139‑1 (MQ=255)
                                                                                                            atggatggACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCATCGACCtgctgc                   >  2:1199937/1‑139 (MQ=255)
                                                                                                            atggatggACAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCATCGACCtgctgc                   >  1:509003/1‑139 (MQ=255)
                                                                                                             tggatggaCAGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCATCGACCTgctgct                  <  2:706531/139‑1 (MQ=255)
                                                                                                                      aGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAAcag                                                                                              >  1:637353/1‑54 (MQ=255)
                                                                                                                      aGGCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAAcag                                                                                              <  2:637353/54‑1 (MQ=255)
                                                                                                                       ggCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCaa        >  1:1347083/1‑139 (MQ=255)
                                                                                                                        gCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAAc       >  1:1482047/1‑139 (MQ=255)
                                                                                                                        gCTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAAc       >  1:1390408/1‑139 (MQ=255)
                                                                                                                         cTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAAt                                                                                                        >  1:1590635/1‑41 (MQ=38)
                                                                                                                         cTGATGAGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAAt                                                                                                        <  2:1590635/41‑1 (MQ=38)
                                                                                                                           gatgaGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGc    <  2:1355110/139‑1 (MQ=255)
                                                                                                                             tgaGCT‑GGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTg  <  1:336042/139‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                     
GGGCGATATGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTGGGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAGGTTTTGACGATAACCGTGGTCTGCGCATCGACCTGCTGCTCGCCAGCCAACCGCTG  >  NC_000913/1832874‑1833138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A