Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I1 R1	776	60.0	3074904	90.1%	2770488	104.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,200,899	A→G	E207E (GAA→GAG)	yehI →	DUF4132 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,200,899	0	A	G	100.0%	199.0 / NA	57	E207E (GAA→GAG)	yehI	DUF4132 domain‑containing protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (27/30); total (27/30)

TTCGATCTCCGACTGCGTAAGCATCTCTCTCTGGCAGAGGAAAGCTGCTGGCAGCGTTGCGCCGACAAACTCATTGCCGCACTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAAAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGCGGTGAGAAAACTCGAACACTACTGGAGCCAGGA  >  NC_000913/2200765‑2201030

ttCGATCTCCGACTGCGTAAGCATCTCTCTCTGGCAGAGGAAAGCTGCTGGCAGCGTTGCGCCGACAAACTCATTGCCGCACTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAAc                                                                                                                                 <  1:761074/139‑1 (MQ=255)

tccGATCTCCGACTGCGTAAGCATCTCTCTCTGGCAGAGGAAAGCTGCTGGCAGCGTTGCGCCGACAAACTCATTGCCGCACTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAAc                                                                                                                                 >  1:508235/3‑139 (MQ=255)

          gACTGCGTAAGCATCTCTCTCTGGCAGAGGAAAGCTGCTGGCAGCGTTGCGCCGACAAACTCATTGCCGCACTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGc                                                                                                                       <  1:460291/139‑1 (MQ=255)

               cGTAAGCATCTCTCTCTGGCAGAGGAAAGCTGCTGGCAGCGTTGCGCCGACAAACTCATTGCCGCACTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATg                                                                                                                  >  2:660981/1‑139 (MQ=255)

               cGTAAGCATCTCTCTCTGGCAGAGGAAAGCTGCTGGCAGCGTTGCGCCGACAAACTCATTGCCGCACTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATg                                                                                                                  <  2:508235/139‑1 (MQ=255)

                                     agGAAAGCTGCTGGCAGCGTTGCGCCGACAAACTCATTGCCGCACTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGccc                                                                                            >  2:328650/1‑139 (MQ=255)

                                         aaGCTGCTGGCAGCGTTGCGCCGACAAACTCATTGCCGCACTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGa                                                                                        <  1:328650/139‑1 (MQ=255)

                                           gctgctGGCAGCGTTGCGCCGACAAACTCATTGCCGCACTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAAc                                                                                      >  1:77831/1‑139 (MQ=255)

                                                 ggCAGCGTTGCGCCGACAAACTCATTGCCGCACTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCAttt                                                                                <  1:231290/139‑1 (MQ=255)

                                                  gCAGCGTTGCGCCGACAAACTCATTGCCGCACTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCAtttt                                                                               <  2:77831/139‑1 (MQ=255)

                                                  gCAGCGTTGCGCCGACAAACTCATTGCCGCACTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCAtttt                                                                               <  1:155253/139‑1 (MQ=255)

                                                       gTTGCGCCGACAAACTCATTGCCGCACTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTgg                                                                                                            >  2:1231044/1‑105 (MQ=255)

                                                       gTTGCGCCGACAAACTCATTGCCGCACTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTgg                                                                                                            <  1:1231044/105‑1 (MQ=255)

                                                         tGCGCCGACAAACTCATTGCCGCACTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTc                                                                              <  2:954115/133‑1 (MQ=255)

                                                         tGCGCCGACAAACTCATTGCCGCACTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTc                                                                              >  1:954115/1‑133 (MQ=255)

                                                             ccGACAAACTCATTGCCGCACTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGa                                                                    <  1:660981/139‑1 (MQ=255)

                                                                                aCTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCACGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCt                                                 >  2:442677/1‑139 (MQ=255)

                                                                                  tACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGCGGTTAAAAGTTGTTGCTaa                                               >  1:1379839/1‑139 (MQ=255)

                                                                                    ccAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCAttt                                                                                >  1:1325275/1‑104 (MQ=255)

                                                                                    ccAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCAttt                                                                                <  2:1325275/104‑1 (MQ=255)

                                                                                            aaaCAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCcaca                                     >  2:550922/1‑139 (MQ=255)

                                                                                            aaaCAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCcaca                                     <  1:1211295/139‑1 (MQ=255)

                                                                                              aCAAAGTTCGTCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGc                                   >  2:1136268/1‑139 (MQ=255)

                                                                                              aCAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATTGCCAATGAGTTGGTAGTCCTTGaa                                                                                                 <  1:938031/77‑1 (MQ=255)

                                                                                              aCAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGaa                                                                                                 >  2:938031/1‑77 (MQ=255)

                                                                                              aCAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCa                                                                             <  1:65552/97‑1 (MQ=255)

                                                                                              aCAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCa                                                                             >  2:65552/1‑97 (MQ=255)

                                                                                              aCAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGc                                   >  2:427157/1‑139 (MQ=255)

                                                                                                  aGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTgg                                                                                                            >  1:1259824/1‑62 (MQ=255)

                                                                                                  aGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTgg                                                                                                            <  2:1259824/62‑1 (MQ=255)

                                                                                                  aGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTc                                                                              >  2:857243/1‑92 (MQ=255)

                                                                                                  aGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTc                                                                              <  1:857243/92‑1 (MQ=255)

                                                                                                  aGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTc                                                                              <  2:1103823/92‑1 (MQ=255)

                                                                                                  aGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTc                                                                              >  1:1103823/1‑92 (MQ=255)

                                                                                                   gTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGa                                                                                                                             <  1:336379/44‑1 (MQ=255)

                                                                                                   gTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGa                                                                                                                             >  2:336379/1‑44 (MQ=255)

                                                                                                       gccgccCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGCGGTGAGaaa                          >  1:613378/1‑139 (MQ=255)

                                                                                                         cgccCTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGTCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGCGGTGAGAAAAc                        <  2:782597/139‑1 (MQ=255)

                                                                                                            ccTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGcc                                                                                                                      >  1:839787/1‑42 (MQ=255)

                                                                                                            ccTTTTATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGcc                                                                                                                      <  2:839787/42‑1 (MQ=255)

                                                                                                              ttttATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGCGGTGAGAAAACTCGaa                   <  1:838232/139‑1 (MQ=255)

                                                                                                                ttATTGCGCTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGCGGTGAGAAAACTCGAaca                 <  1:550922/139‑1 (MQ=255)

                                                                                                                      cgcTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGCGGTGAGAAAACTCGAACACTACTg           >  2:342095/1‑139 (MQ=255)

                                                                                                                       gcTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGCGGTGAGAAAACTCGAACACTACTgg          >  1:1411077/1‑139 (MQ=255)

                                                                                                                        cTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACt                                                                                     <  2:263464/63‑1 (MQ=255)

                                                                                                                        cTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACt                                                                                     >  1:263464/1‑63 (MQ=255)

                                                                                                                        cTGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGCGGTGAGAAAACTCGAACACTACTGGa         >  2:225426/1‑139 (MQ=255)

                                                                                                                         tGATCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTTATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGCGGTGAGAAAACTCGAACACTACTGGAg        <  1:427157/139‑1 (MQ=255)

                                                                                                                           aTCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGa                                            <  2:255753/101‑1 (MQ=255)

                                                                                                                           aTCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGa                                            >  1:255753/1‑101 (MQ=255)

                                                                                                                           aTCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGCGGTGAGAAAACTCGAACACTACTGGAGcc      <  1:1246801/139‑1 (MQ=255)

                                                                                                                           aTCCTCCCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGCGGTGAGAAAACTCGAACACTACTGGAGcc      <  1:1136268/139‑1 (MQ=255)

                                                                                                                               tccCGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGCGGTGAGAAAACTCGAACACTACTGGAGCCAGGa  <  2:1379839/139‑1 (MQ=255)

                                                                                                                                cccGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGCGGTGAGAAAACTCGAACACTACTGGAg        <  2:1107942/132‑1 (MQ=255)

                                                                                                                                cccGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGCGGTGAGAAAACTCGAACACTACTGGAg        <  1:172741/132‑1 (MQ=255)

                                                                                                                                cccGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGCGGTGAGAAAACTCGAACACTACTGGAg        >  2:172741/1‑132 (MQ=255)

                                                                                                                                cccGGAGAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGCGGTGAGAAAACTCGAACACTACTGGAg        >  1:1107942/1‑132 (MQ=255)

TTCGATCTCCGACTGCGTAAGCATCTCTCTCTGGCAGAGGAAAGCTGCTGGCAGCGTTGCGCCGACAAACTCATTGCCGCACTACCAGGAATAAACAAAGTTCGCCGCCCTTTTATTGCGCTGATCCTCCCGGAAAAACCAGAAATAGCCAATGAGTTGGTAGGCCTTGAATGCCCGCGAACTCATTTTCATTCTAAGGAGTGGTTAAAAGTTGTTGCTAATGACCCCACAGCGGTGAGAAAACTCGAACACTACTGGAGCCAGGA  >  NC_000913/2200765‑2201030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A