Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F1 I1 R1
|
776 |
60.0 |
3074904 |
90.1% |
2770488 |
104.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,276,298 |
G→A |
*342* (TAG→TAA) |
yejE → |
microcin C ABC transporter permease |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,276,298 | 0 | G | A | 98.1%
| 163.3
/ NA
| 52 | *342* (TAG→TAA) | yejE | microcin C ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (23/28); minor base C (0/1); total (23/29) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTC > NC_000913/2276163‑2276430
|
gTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACAt < 1:426065/139‑1 (MQ=255)
ttACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATg > 1:583101/1‑139 (MQ=255)
gggAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCaa < 1:259278/139‑1 (MQ=255)
ggAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCaaa > 2:298589/1‑139 (MQ=255)
aCCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAg < 1:298589/139‑1 (MQ=255)
cTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCg > 1:1020197/1‑139 (MQ=255)
ttCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGa < 1:182699/139‑1 (MQ=255)
ccGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCa > 2:464667/1‑117 (MQ=255)
ccGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCa < 1:464667/117‑1 (MQ=255)
ccGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAAt < 2:1020197/139‑1 (MQ=255)
ccGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCaaa < 1:1105883/103‑1 (MQ=255)
ccGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCaaa > 2:1105883/1‑103 (MQ=255)
gCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGc < 2:1019337/139‑1 (MQ=255)
accTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGACGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTACCATGACGCAAACGCTGTTAGCGATTGAGAATTTGTCGGTGGGTTTTCGc < 2:271822/138‑1 (MQ=255)
tcttGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATc > 1:316810/1‑139 (MQ=255)
gTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCa > 2:1308255/1‑139 (MQ=255)
tCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCaa < 1:1308255/139‑1 (MQ=255)
ggTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAAc < 1:844802/139‑1 (MQ=255)
tattatTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgtacgta < 2:366780/139‑1 (MQ=255)
attattGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCgtacgtac < 2:316810/139‑1 (MQ=255)
ttattGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACa < 2:1116977/139‑1 (MQ=255)
tttGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGttt > 1:352804/1‑106 (MQ=255)
tttGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGttt < 2:352804/106‑1 (MQ=255)
tGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGc > 2:986137/1‑115 (MQ=255)
tGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGc < 1:986137/115‑1 (MQ=255)
gCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatga < 2:583101/139‑1 (MQ=255)
tCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGttt > 1:747580/1‑139 (MQ=255)
tATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCa < 1:956584/58‑1 (MQ=255)
tATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCa > 2:956584/1‑58 (MQ=255)
gAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGAtt > 1:769185/1‑139 (MQ=255)
aaGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgt < 1:231298/104‑1 (MQ=255)
aaGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgt > 2:231298/1‑104 (MQ=255)
tCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACa > 1:425804/1‑105 (MQ=255)
tCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACa < 2:425804/105‑1 (MQ=255)
ccGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCaa > 1:771889/1‑92 (MQ=255)
ccGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCaa < 2:771889/92‑1 (MQ=255)
ccGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTgg > 2:563828/1‑139 (MQ=255)
cgcgACGCATTTGATCCTAATATGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatga < 2:1497808/114‑1 (MQ=255)
cgcgACGCATTTGATCCTAATATGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatga > 1:1497808/1‑114 (MQ=255)
gcgACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCg < 2:769185/139‑1 (MQ=255)
gCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAAcg < 2:747580/139‑1 (MQ=255)
taataaGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCtggt > 2:128471/1‑139 (MQ=255)
taaGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTTACTACAGATTGAGGCTGGCGAAACGCTGGCGCTggtggg < 1:128471/139‑1 (MQ=255)
ggCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAAc > 1:64810/1‑70 (MQ=255)
ggCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAAc < 2:64810/70‑1 (MQ=255)
ggCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACa < 2:1388016/80‑1 (MQ=255)
ggCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACa > 1:1388016/1‑80 (MQ=255)
ggCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGa > 2:181776/1‑139 (MQ=255)
cGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACa < 2:1079805/78‑1 (MQ=255)
cGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACa > 1:1079805/1‑78 (MQ=255)
ggTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTc > 2:1033392/1‑139 (MQ=255)
gtAACATGACGCAAACTCTGTTAGCGATTGAAAAt < 1:155022/35‑1 (MQ=255)
|
GTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTC > NC_000913/2276163‑2276430
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
