Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F1 I1 R1
|
770 |
68.5 |
3470224 |
91.7% |
3182195 |
103.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,276,530 |
G→A |
S77S (TCG→TCA) |
yejF → |
microcin C ABC transporter ATPase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,276,530 | 0 | G | A | 96.5%
| 171.4
/ ‑1.7
| 57 | S77S (TCG→TCA) | yejF | microcin C ABC transporter ATPase |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (25/30); total (26/31) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.22e-01 |
AGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCGCTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGGTGTCGTTAAATCCATTGCATACCCTGGAAAAACAGCTTTATGAAGTGCTTTCACT > NC_000913/2276397‑2276661
|
aGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCactgct < 2:589790/139‑1 (MQ=255)
cTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCa > 2:1165755/1‑139 (MQ=255)
ggCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACg > 1:236490/1‑139 (MQ=255)
gCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGc > 2:1458324/1‑139 (MQ=255)
cGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGcc < 2:236490/139‑1 (MQ=255)
ggcgctggTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCaa > 2:937807/1‑139 (MQ=255)
tgggtgAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTgcg < 1:878886/139‑1 (MQ=255)
tgggtgAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTgcg < 1:937807/139‑1 (MQ=255)
cAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTAcg < 1:1715865/139‑1 (MQ=255)
gTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCgg > 1:5819/1‑139 (MQ=255)
gCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGtaataa > 1:1524674/1‑139 (MQ=255)
aaGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGcc > 2:1643713/1‑100 (MQ=255)
aaGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGcc < 1:1643713/100‑1 (MQ=255)
gcTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGAttttt < 1:1458324/139‑1 (MQ=255)
tGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCa < 2:1027755/139‑1 (MQ=255)
tCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAgg < 2:343130/139‑1 (MQ=255)
gCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTAcg > 2:782115/1‑100 (MQ=255)
gCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTAcg < 1:782115/100‑1 (MQ=255)
gCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGgt > 1:846208/1‑139 (MQ=255)
ccTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGgtg < 1:251943/139‑1 (MQ=255)
cTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTAcg < 2:1452673/98‑1 (MQ=255)
cTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTAcg > 1:1452673/1‑98 (MQ=255)
cTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGAttttt < 1:997995/123‑1 (MQ=255)
cTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGAttttt > 2:997995/1‑123 (MQ=255)
gCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATc > 1:880510/1‑77 (MQ=255)
gCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATc < 2:880510/77‑1 (MQ=255)
gCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGGTGTCg > 2:436325/1‑139 (MQ=255)
cTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGGTGTCGt > 2:465382/1‑139 (MQ=255)
ttCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGgtg > 1:1155066/1‑130 (MQ=255)
ttCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCATCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGgtg < 2:1155066/130‑1 (MQ=255)
ccccGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGAt < 1:1459071/100‑1 (MQ=255)
ccccGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGAt > 2:1459071/1‑100 (MQ=255)
cccGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGGTGTCGTTAAATCCa < 2:1062003/139‑1 (MQ=255)
ccgGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGATTCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGGTGTCGTTAAATCCATTGc < 2:1038206/139‑1 (MQ=255)
ccgGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCACGAACCGATGGTGTCGTTAAATCCATTGc > 1:1038206/1‑139 (MQ=255)
ggTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCGCTGCTTCACGCCAGCGATCAAACGtt < 1:725742/69‑1 (MQ=255)
ggTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCGCTGCTTCACGCCAGCGATCAAACGtt > 2:725742/1‑69 (MQ=255)
gTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCa < 2:1552293/107‑1 (MQ=255)
gTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCa > 1:1552293/1‑107 (MQ=255)
gAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTgcgc > 1:1414132/1‑69 (MQ=255)
gAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTgcgc < 2:1414132/69‑1 (MQ=255)
ctcCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTAcg > 1:783739/1‑69 (MQ=255)
ctcCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTAcg < 2:783739/69‑1 (MQ=255)
ctcCGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGGTGTCGTTAAATCCATTGCATACCCTGGaaaaa < 2:73484/139‑1 (MQ=255)
ctcCGGCGATATTCGTTTGCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGGTGTCGTTAAATCCATTGCATACCCTGGaaaaa < 1:1165755/139‑1 (MQ=255)
cGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGAt < 1:1326037/87‑1 (MQ=255)
cGGCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGAt > 2:1326037/1‑87 (MQ=255)
gCGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGGTGTCGTTAAATCCATTGCATACCCTGGAAAAACAGCt < 1:436325/139‑1 (MQ=255)
cGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGgt > 1:79623/1‑102 (MQ=255)
cGATATTCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGgt < 2:79623/102‑1 (MQ=255)
ttCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTAcg > 1:385327/1‑58 (MQ=255)
ttCGTTTTCATGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTAcg < 2:385327/58‑1 (MQ=255)
tGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGGTGTCGTTaa > 2:476476/1‑95 (MQ=255)
tGGCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGGTGTCGTTaa < 1:476476/95‑1 (MQ=255)
ggCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTAc > 2:903831/1‑46 (MQ=255)
ggCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTAc < 1:903831/46‑1 (MQ=255)
gCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGGTGTCGTTAAATCCATTGCATACCCTGGAAAAACAGCTTTATGAAGTGCTTTCACt < 1:465382/139‑1 (MQ=255)
gCGAATCACTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGGTGTCGTTAAATCCATTGCATACCCTGGAAAAACAGCTTTATGAAGTGCTTTCACt < 2:5819/139‑1 (MQ=255)
|
AGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGGTTCAGGCAAAAGCGTTACCGCGCTGTCAATTTTACGCCTGCTCCCTTCCCCGCCGGTTGAATATCTCTCCGGCGATATTCGTTTTCATGGCGAATCGCTGCTTCACGCCAGCGATCAAACGTTGCGCGGTGTACGCGGTAATAAGATCGCCATGATTTTTCAGGAACCGATGGTGTCGTTAAATCCATTGCATACCCTGGAAAAACAGCTTTATGAAGTGCTTTCACT > NC_000913/2276397‑2276661
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
