Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I1 R1	776	60.0	3074904	90.1%	2770488	104.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,285,401	+G	coding (1026/1761 nt)	yejM →	essential inner membrane DUF3413 domain‑containing protein, lipid A production and membrane permeability factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,285,397	1	.	G	100.0%	169.5 / NA	49	L341R (CTG→CGG)	yejM	essential inner membrane DUF3413 domain‑containing protein, lipid A production and membrane permeability factor
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (25/24); total (25/24)

ATATGAGCTCCGGCAACACTACAGACAACGGCATCTTTGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCT‑GGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAGCAGACCGCCACGCAGTGGATCAACTGGCTGGG  >  NC_000913/2285259‑2285526

atatGAGCTCCGGCAACACTACAGACAACGGCATCTTTGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCt                                                                                                                                    <  2:221318/139‑1 (MQ=255)

atatGAGCTCCGGCAACACTACAGACAACAGCATCTTTGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCTGCt                                                                                                                                    <  2:989780/139‑1 (MQ=255)

         ccGGCAACACTACAGACAACGGCATCTTTGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCT‑gggggttat                                                                                                                          >  2:24398/1‑134 (MQ=255)

                  cTACAGACAATGGCATCTTTGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAg                                                                                                                  <  2:748395/139‑1 (MQ=255)

                  cTACAGACAACGGCATCTTTGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAg                                                                                                                  <  1:273638/139‑1 (MQ=255)

                  cTACAGACAACGGCATCTTTGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAg                                                                                                                  <  2:1256129/139‑1 (MQ=255)

                    aCAGACAACGGCATCTTTGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGAt                                                                                                                >  1:124159/1‑139 (MQ=255)

                          aaCGGCATCTTTGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCttt                                                                                                          >  2:1320865/1‑139 (MQ=255)

                            cGGCATCTTTGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGAGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTAc                                                                                                        >  2:878890/1‑139 (MQ=255)

                               cATCTTTGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATACTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCT‑ggggg                                                                                                                              <  1:1223376/113‑2 (MQ=255)

                               cATCTTTGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATACTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCT‑ggggg                                                                                                                              >  2:1223376/1‑112 (MQ=255)

                                     tGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCt                                                                                               <  1:878890/139‑1 (MQ=255)

                                       gCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGt                                                                                             <  2:1304279/139‑1 (MQ=255)

                                          tGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATc                                                                                          <  1:1320865/139‑1 (MQ=255)

                                            ttCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGc                                                                                        >  1:1509682/1‑139 (MQ=255)

                                                      tctcGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCAttgt                                                                              >  2:1329466/1‑139 (MQ=255)

                                                      tctcGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCAttgt                                                                              >  2:1500021/1‑139 (MQ=255)

                                                      tctcGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCAttgt                                                                              >  2:1094491/1‑139 (MQ=255)

                                                      tctcGCCGAGCTATATGGACGGCATTCTGTCGACCCGTAAGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGATGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCAttgt                                                                              >  1:1335128/1‑139 (MQ=255)

                                                             gAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGa                                                                       <  1:24398/139‑1 (MQ=255)

                                                                  atatGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCg                                                                                         <  2:182938/116‑1 (MQ=255)

                                                                  atatGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCg                                                                                         >  1:182938/1‑116 (MQ=255)

                                                                       gACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATg                                                             <  2:162016/139‑1 (MQ=255)

                                                                          ggCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAg                                                                                                                  <  1:51468/83‑1 (MQ=255)

                                                                          ggCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAg                                                                                                                  >  2:51468/1‑83 (MQ=255)

                                                                          ggCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCg                                                          >  1:1433988/1‑139 (MQ=255)

                                                                          ggCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCg                                                          >  1:1199983/1‑139 (MQ=255)

                                                                             aTTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCTGCTGTATCGCCAGGCa                                                                                  >  2:1218616/1‑112 (MQ=255)

                                                                             aTTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCTGCTGTATCGCCAGGCa                                                                                  <  1:1218616/112‑1 (MQ=255)

                                                                                   tCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGc                                                 <  1:1094491/139‑1 (MQ=255)

                                                                                           tACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATgg                                                                                                              <  2:821426/70‑1 (MQ=255)

                                                                                           tACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATgg                                                                                                              >  1:821426/1‑70 (MQ=255)

                                                                                                 tGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAg                                                                                                                  <  1:384518/60‑1 (MQ=255)

                                                                                                 tGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAg                                                                                                                  >  2:384518/1‑60 (MQ=255)

                                                                                                        ttaattaCTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATc                                                                                          >  2:615474/1‑77 (MQ=255)

                                                                                                        ttaattaCTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATc                                                                                          <  1:615474/77‑1 (MQ=255)

                                                                                                         taattaCTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAGCAGACCg                           >  1:368551/1‑139 (MQ=255)

                                                                                                           attaCTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAGCAGACCGcc                         <  1:1329466/139‑1 (MQ=255)

                                                                                                               cTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAGCAGACCGCCACGc                     <  1:1500021/139‑1 (MQ=255)

                                                                                                               cTGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAGCAGACCGCCACGc                     <  2:368551/139‑1 (MQ=255)

                                                                                                                tGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCa                                                                                  <  2:588517/77‑1 (MQ=255)

                                                                                                                tGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCa                                                                                  >  1:588517/1‑77 (MQ=255)

                                                                                                                tGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAg                                  <  1:1203534/125‑1 (MQ=255)

                                                                                                                tGCGCTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAg                                  >  2:1203534/1‑125 (MQ=255)

                                                                                                                  cgcTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTAc                                                   >  1:463621/1‑106 (MQ=255)

                                                                                                                  cgcTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTAc                                                   <  2:463621/106‑1 (MQ=255)

                                                                                                                  cgcTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAGCAGACCGCCACGCAGt                  >  2:140806/1‑139 (MQ=255)

                                                                                                                    cTTAATCAGCGAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAGCAGACCGCCACGCAGTgg                >  1:50166/1‑139 (MQ=255)

                                                                                                                    cTTAATCAGCAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAGCAGACCGCCACGCACTgg                >  1:818227/1‑139 (MQ=255)

                                                                                                                             cAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGa                                                                       <  2:83659/75‑1 (MQ=255)

                                                                                                                             cAAGGCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGa                                                                       >  1:83659/1‑75 (MQ=255)

                                                                                                                                 gCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTa                                                                                            <  1:1308186/50‑1 (MQ=255)

                                                                                                                                 gCTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTa                                                                                            >  2:1308186/1‑50 (MQ=255)

                                                                                                                                  cTATCAGCTGGGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAGCAGACCGCCACGCAGTGGATCAACTGGCTggg  <  2:1199983/139‑1 (MQ=255)

ATATGAGCTCCGGCAACACTACAGACAACGGCATCTTTGGCCTGTTCTATGGCATCTCGCCGAGCTATATGGACGGCATTCTGTCGACCCGTACGCCTGCGGCATTAATTACTGCGCTTAATCAGCAAGGCTATCAGCT‑GGGGTTATTCTCATCAGATGGCTTTACCAGCCCGCTGTATCGCCAGGCATTGTTGTCAGATTTCTCGATGCCGAGCGTACGCACCCAATCCGACGAGCAGACCGCCACGCAGTGGATCAACTGGCTGGG  >  NC_000913/2285259‑2285526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A