Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I1 R1	776	60.0	3074904	90.1%	2770488	104.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,331,634	C→T	A378V (GCG→GTG)	proP →	proline/glycine betaine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,331,634	0	C	T	100.0%	197.3 / NA	61	A378V (GCG→GTG)	proP	proline/glycine betaine transporter
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (31/30); total (31/30)

CCTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTA  >  NC_000913/4331503‑4331769

ccTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTc                                                                                                                                  >  1:316568/1‑139 (MQ=255)

    tttGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGa                                                                                                                              >  1:75241/1‑139 (MQ=255)

    tttGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGa                                                                                                                              >  1:1401290/1‑139 (MQ=255)

      tgtgTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACg                                                                                                                            >  1:1502448/1‑139 (MQ=255)

       gtgtTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGc                                                                                                                           <  2:75241/139‑1 (MQ=255)

         gtTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCat                                                                                                                         <  2:1114452/139‑1 (MQ=255)

              cGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGa                                                                                                                              <  2:391583/129‑1 (MQ=255)

              cGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGa                                                                                                                              >  1:391583/1‑129 (MQ=255)

              cGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCg                                                                                                                    <  2:1401290/139‑1 (MQ=255)

                   ccGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCat                                                                                                                         <  1:1494023/129‑1 (MQ=255)

                   ccGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCat                                                                                                                         >  2:1494023/1‑129 (MQ=255)

                        gTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAgcgcg                                                                                                          <  1:575551/139‑1 (MQ=255)

                        gTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAgcgcg                                                                                                          <  2:189573/139‑1 (MQ=255)

                                 gATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcggcg                                                                                                 >  2:76439/1‑139 (MQ=255)

                                 gATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcggcg                                                                                                 <  2:1502448/139‑1 (MQ=255)

                                             cGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATAtt                                                                                     <  2:316568/139‑1 (MQ=255)

                                                cATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCatat                                                                                                                       <  1:34632/102‑1 (MQ=255)

                                                cATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCatat                                                                                                                       >  2:34632/1‑102 (MQ=255)

                                                cATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCg                                                                                  <  1:76439/139‑1 (MQ=255)

                                                    ggCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTg                                                                               >  1:446367/1‑138 (MQ=255)

                                                    ggCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTg                                                                               <  2:446367/138‑1 (MQ=255)

                                                        tGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGt                                                                          >  1:80019/1‑139 (MQ=255)

                                                                    ggTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCAtt                                                                                            <  1:1099167/109‑1 (MQ=255)

                                                                    ggTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCAtt                                                                                            >  2:1099167/1‑109 (MQ=255)

                                                                    ggTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGAc                                                              >  1:208877/1‑139 (MQ=255)

                                                                               cTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGa                                                                                                                              <  1:1487078/64‑1 (MQ=255)

                                                                               cTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGa                                                                                                                              >  2:1487078/1‑64 (MQ=255)

                                                                               cTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTgg                                                   >  2:1455445/1‑139 (MQ=255)

                                                                                 ggCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTccc                                                                                                                                >  1:366329/1‑60 (MQ=255)

                                                                                 ggCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTccc                                                                                                                                <  2:366329/60‑1 (MQ=255)

                                                                                    ggTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGcc                                              >  1:202883/1‑139 (MQ=255)

                                                                                        aTCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCat                                                                                                                         >  1:783825/1‑60 (MQ=255)

                                                                                        aTCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCat                                                                                                                         <  2:783825/60‑1 (MQ=255)

                                                                                              aaCTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCg                                    >  2:275278/1‑139 (MQ=255)

                                                                                                  gCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATAttt                                                                                    >  1:919115/1‑87 (MQ=255)

                                                                                                  gCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATAttt                                                                                    <  2:919115/87‑1 (MQ=255)

                                                                                                  gCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAg                                >  1:1048816/1‑139 (MQ=255)

                                                                                                   cTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTcc                                                                                                                                 <  2:777104/41‑1 (MQ=255)

                                                                                                   cTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTcc                                                                                                                                 >  1:777104/1‑41 (MQ=255)

                                                                                                   cTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCat                                                                                                                         >  1:879577/1‑49 (MQ=255)

                                                                                                   cTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCa                                                                                              >  2:1225936/1‑76 (MQ=255)

                                                                                                   cTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCa                                                                                              <  1:1225936/76‑1 (MQ=255)

                                                                                                   cTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGc                               >  2:1501608/1‑139 (MQ=255)

                                                                                                   cTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGc                               >  2:912527/1‑139 (MQ=255)

                                                                                                   cTTTACGGGCGTTATGGCTTCTACCTTGCCACTGATGTTCCCGACGCat                                                                                                                         <  2:879577/49‑1 (MQ=255)

                                                                                                    tttACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGCGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCt                              >  2:1194634/1‑139 (MQ=255)

                                                                                                     ttACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAgcg                                                                                                            <  2:1290977/60‑1 (MQ=255)

                                                                                                     ttACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAgcg                                                                                                            >  1:1290977/1‑60 (MQ=255)

                                                                                                     ttACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTc                             >  1:1330856/1‑139 (MQ=255)

                                                                                                      tACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCg                            <  2:208877/139‑1 (MQ=255)

                                                                                                       aCGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGc                           <  1:275278/139‑1 (MQ=255)

                                                                                                        cGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGCGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCa                          <  1:1194634/139‑1 (MQ=255)

                                                                                                             gTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTg                                                    <  1:154810/108‑1 (MQ=255)

                                                                                                             gTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTg                                                    >  2:154810/1‑108 (MQ=255)

                                                                                                                aTGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCctgg                                           <  2:952086/114‑1 (MQ=255)

                                                                                                                aTGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCctgg                                           >  1:952086/1‑114 (MQ=255)

                                                                                                                  ggCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCATCGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCtgatg                <  2:947125/139‑1 (MQ=255)

                                                                                                                      tCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGAtgc            <  2:1048816/139‑1 (MQ=255)

                                                                                                                        tACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGAtgcct          <  2:762348/139‑1 (MQ=255)

                                                                                                                         aCCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGAtgcctg         >  2:267871/1‑139 (MQ=255)

                                                                                                                                cAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTa  <  2:202883/139‑1 (MQ=255)

CCTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTA  >  NC_000913/4331503‑4331769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A