Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I2 R1	764	42.0	2018750	95.5%	1927906	109.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,331,634	C→T	A378V (GCG→GTG)	proP →	proline/glycine betaine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,331,634	0	C	T	100.0%	94.0 / NA	30	A378V (GCG→GTG)	proP	proline/glycine betaine transporter
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (16/14); total (16/14)

CCCTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTAT  >  NC_000913/4331502‑4331767

cccTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGtt                                                                                                                                 >  2:510783/1‑139 (MQ=255)

    gTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCg                                                                                                                             <  1:510783/139‑1 (MQ=255)

            tGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCatat                                                                                                                     <  2:843385/139‑1 (MQ=255)

                  tCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTa                                                                                                               <  1:751586/139‑1 (MQ=255)

                            tATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGc                                                                                                                         >  2:531797/1‑119 (MQ=255)

                            tATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGc                                                                                                                         <  1:531797/119‑1 (MQ=255)

                                 tGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcggc                                                                                                <  1:104411/139‑1 (MQ=255)

                                   aTTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGAt                                                                                                                                    >  2:866437/1‑101 (MQ=255)

                                   aTTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGAt                                                                                                                                    <  1:866437/101‑1 (MQ=255)

                                             aCGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTcc                                                                                                                               >  2:175653/1‑96 (MQ=255)

                                             aCGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTcc                                                                                                                               <  1:175653/96‑1 (MQ=255)

                                              cGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCAGTTACAGCGCGCTggcggcg                                                                                               <  1:28693/127‑1 (MQ=255)

                                              cGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCAGTTACAGCGCGCTggcggcg                                                                                               >  2:28693/1‑127 (MQ=255)

                                                    cGGCCTGATTTTTGCCGGATTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTg                                                                             >  1:561780/1‑139 (MQ=255)

                                                                                  ggCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcgg                                                                                                 >  1:58155/1‑89 (MQ=255)

                                                                                  ggCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcgg                                                                                                 <  2:58155/89‑1 (MQ=255)

                                                                                    cGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCt                                                                           <  2:614966/109‑1 (MQ=255)

                                                                                    cGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCt                                                                           >  1:614966/1‑109 (MQ=255)

                                                                                                aCTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGa                                 <  2:561780/139‑1 (MQ=255)

                                                                                                   gCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAg                              >  2:502397/1‑139 (MQ=255)

                                                                                                    cTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGc                             >  1:204223/1‑139 (MQ=255)

                                                                                                    cTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGc                             >  2:774691/1‑139 (MQ=255)

                                                                                                       tACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCg                          <  1:774691/139‑1 (MQ=255)

                                                                                                                       tCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGATCGAAAGCTCGCAGAATCTGATGAtgc          <  2:204223/139‑1 (MQ=255)

                                                                                                                        cTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGAtgcc         >  1:632648/1‑139 (MQ=255)

                                                                                                                              tGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAGGCTCGCAGAATCTGATGATGCCTGCCTa   >  2:606587/1‑139 (MQ=255)

                                                                                                                              tGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTa   >  1:186840/1‑139 (MQ=255)

                                                                                                                               gCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGt                                                                 >  1:200725/1‑76 (MQ=255)

                                                                                                                               gCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGt                                                                 <  2:200725/76‑1 (MQ=255)

                                                                                                                               gCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTAt  >  2:222800/1‑139 (MQ=255)

CCCTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTAT  >  NC_000913/4331502‑4331767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A