Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I1 R1	759	71.0	3673240	91.5%	3361014	104.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	385,737	A→G	D169G (GAT→GGT)	tauA →	taurine ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	385,737	0	A	G	96.5%	186.3 / ‑2.7	57	D169G (GAT→GGT)	tauA	taurine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (28/27); total (29/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.21e-01

CGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATT  >  NC_000913/385601‑385868

cGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGgta                                                                                                                                   <  2:210046/139‑1 (MQ=255)

               tttATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATg                                                                                                                    >  2:970184/1‑139 (MQ=255)

                 tATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTc                                                                                                                  >  2:1814005/1‑139 (MQ=255)

                    ctcCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATg                                                                                                                             <  2:1646753/125‑1 (MQ=255)

                    ctcCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATg                                                                                                                             >  1:1646753/1‑125 (MQ=255)

                            cccacTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGa                                                                                                                               >  2:878468/1‑115 (MQ=255)

                            cccacTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGa                                                                                                                               <  1:878468/115‑1 (MQ=255)

                            cccacTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATg                                                                                                                             >  2:1118871/1‑117 (MQ=255)

                            cccacTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATg                                                                                                                             <  1:1118871/117‑1 (MQ=255)

                               acTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGt                                                                                                    >  1:237134/1‑139 (MQ=255)

                                 tACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGa                                                                                                                               <  1:1566957/110‑1 (MQ=255)

                                 tACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGa                                                                                                                               >  2:1566957/1‑110 (MQ=255)

                                 tACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTa                                                                                                  <  1:233920/139‑1 (MQ=255)

                                 tACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTa                                                                                                  <  1:416552/139‑1 (MQ=255)

                                    aGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACg                                                                                               >  1:139333/1‑139 (MQ=255)

                                      ccTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGa                                                                                                                               >  2:1799076/1‑105 (MQ=255)

                                      ccTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGa                                                                                                                               <  1:1799076/105‑1 (MQ=255)

                                      ccTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGcc                                                                                             <  1:970184/139‑1 (MQ=255)

                                      ccTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGcc                                                                                             >  1:378045/1‑139 (MQ=255)

                                       ctgctgGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGccc                                                                                            <  2:615241/139‑1 (MQ=255)

                                        tgctgGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCt                                                                                           >  1:567645/1‑139 (MQ=255)

                                         gctgGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTg                                                                                          >  1:1735210/1‑139 (MQ=255)

                                           tgGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGa                                                                                        >  2:1193324/1‑139 (MQ=255)

                                           tgGCGGCACTGAAACAATGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGGATTGATGGTGCTTATGTCTGGGCACCGACGGTTAACGCCCTGGa                                                                                        >  1:607609/1‑139 (MQ=255)

                                             gcggcACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGaaa                                                                                      >  2:1072796/1‑139 (MQ=255)

                                              cggcACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGaaaa                                                                                     <  1:1814005/139‑1 (MQ=255)

                                               ggcACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTa                                                                                                  >  1:494649/1‑125 (MQ=255)

                                               ggcACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTa                                                                                                  <  2:494649/125‑1 (MQ=255)

                                                              gggCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGa                                                                                                                               <  1:479358/81‑1 (MQ=255)

                                                              gggCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGa                                                                                                                               >  2:479358/1‑81 (MQ=255)

                                                                gCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGAc                                                                   <  2:237134/139‑1 (MQ=255)

                                                                gCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGAc                                                                   >  1:1264814/1‑139 (MQ=255)

                                                                         ccGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGa                                                          <  2:1735210/139‑1 (MQ=255)

                                                                                aGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTc                                                   <  2:1694317/139‑1 (MQ=255)

                                                                                           tgAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGa                                                                                                                               <  1:1566362/52‑1 (MQ=255)

                                                                                           tgAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGa                                                                                                                               >  2:1566362/1‑52 (MQ=255)

                                                                                           tgAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCAc                                                                                                           >  2:692666/1‑72 (MQ=255)

                                                                                           tgAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCAc                                                                                                           <  1:692666/72‑1 (MQ=255)

                                                                                           tgAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGc                                                                                              <  2:900763/85‑1 (MQ=255)

                                                                                           tgAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGc                                                                                              >  1:900763/1‑85 (MQ=255)

                                                                                           tgAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAg                                             <  2:166411/134‑1 (MQ=255)

                                                                                           tgAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAg                                             >  1:166411/1‑134 (MQ=255)

                                                                                           tgAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTgggg                                        <  2:139333/139‑1 (MQ=255)

                                                                                                 tGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACcgg                                                                                                        <  1:1795378/69‑1 (MQ=255)

                                                                                                 tGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACcgg                                                                                                        >  2:1795378/1‑69 (MQ=255)

                                                                                                  gCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGaa                                                         <  2:212654/115‑1 (MQ=255)

                                                                                                  gCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGaa                                                         >  1:212654/1‑115 (MQ=255)

                                                                                                     gccgccCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTTACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACg                              >  1:866212/1‑139 (MQ=255)

                                                                                                          ccgcGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAGGACGGCAAGGTGTTGACCGATTCTGAACAGGTc                                                   >  2:267019/1‑113 (MQ=255)

                                                                                                          ccgcGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAGGACGGCAAGGTGTTGACCGATTCTGAACAGGTc                                                   <  1:267019/113‑1 (MQ=255)

                                                                                                          ccgcGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGa                         <  2:378045/139‑1 (MQ=255)

                                                                                                          ccgcGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGa                         <  2:567645/139‑1 (MQ=255)

                                                                                                          ccgcGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGa                         <  2:1264814/139‑1 (MQ=255)

                                                                                                                tacctcccgCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTg                   >  1:1440409/9‑139 (MQ=255)

                                                                                                                   tCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGAc                                                                   <  2:574820/88‑1 (MQ=255)

                                                                                                                   tCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGAc                                                                   >  1:574820/1‑88 (MQ=255)

                                                                                                                             gCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCaaa      >  1:910581/1‑139 (MQ=255)

                                                                                                                                 cGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGAtt  <  1:1724487/139‑1 (MQ=255)

CGCATCGCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATT  >  NC_000913/385601‑385868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A