Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I1 R1	760	58.2	3022526	90.8%	2744453	102.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	385,737	A→G	D169G (GAT→GGT)	tauA →	taurine ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	385,737	0	A	G	100.0%	105.2 / NA	30	D169G (GAT→GGT)	tauA	taurine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (15/15); total (15/15)

ACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGC‑GGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTT  >  NC_000913/385612‑385870

aCCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGC‑GGGGAGGTATTGATGGTGCt                                                                                                                          <  2:786624/139‑1 (MQ=255)

          tcCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGC‑GGGGAGGTATTGATGGTGCTTATGTCTggg                                                                                                                <  2:1320212/139‑1 (MQ=255)

             accaccCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGC‑GGGGAGGTATTGATGGTGCTTATGTCTGGGCAc                                                                                                             <  1:94007/139‑1 (MQ=255)

                 cccacTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGC‑GGGGAGGTATTGATGGTGCTTATGTCTGGGCACcggc                                                                                                         >  2:967005/1‑139 (MQ=255)

                 cccacTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGC‑GGGGAGGTATTGATGGTGCTTATGTCTGGGCACcgg                                                                                                          >  2:364837/1‑138 (MQ=255)

                 cccacTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGC‑GGGGAGGTATTGATGGTGCTTATGTCTGGGCACcgg                                                                                                          <  1:364837/138‑1 (MQ=255)

                      tACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGC‑GGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTa                                                                                                    >  2:493764/1‑139 (MQ=255)

                            ctgctgGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGC‑GGGGAGGTATTTATGGTGCTTATGTCTGGGCACCGGCGGTTAACGccc                                                                                              >  1:947806/1‑139 (MQ=255)

                             tgctgGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGC‑GGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCt                                                                                             <  2:744964/139‑1 (MQ=255)

                                           aaaCACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGC‑GGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCa                                                                               <  2:545681/139‑1 (MQ=255)

                                                                gggCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGC‑GGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAAc                                                          >  2:844615/1‑139 (MQ=255)

                                                                gggCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGC‑GGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAAc                                                          >  1:671253/1‑139 (MQ=255)

                                                                 ggCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGC‑GGGGAGGTATTGATGGTGCTTATGTCTGGGCAc                                                                                                             >  1:766587/1‑87 (MQ=255)

                                                                 ggCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGC‑GGGGAGGTATTGATGGTGCTTATGTCTGGGCAc                                                                                                             <  2:766587/87‑1 (MQ=255)

                                                                     aGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGC‑GGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTc                                                     <  2:671253/139‑1 (MQ=255)

                                                                                    ccTGCAGCCGCCCGCGATTATCGCGGCCTGGCAGC‑GGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGgcgcg                                      <  1:844615/139‑1 (MQ=255)

                                                                                       gCAGCCGCCCGCGATTATCGCTGCCTGGCAGC‑GGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGc                                                                                                >  1:822959/1‑78 (MQ=255)

                                                                                       gCAGCCGCCCGCGATTATCGCTGCCTGGCAGC‑GGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGc                                                                                                <  2:822959/78‑1 (MQ=255)

                                                                                          gccgccCGCGATTATCGCTGCCTGGCAGC‑GGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGAc                                                                     >  2:1235446/1‑102 (MQ=255)

                                                                                          gccgccCGCGATTATCGCTGCCTGGCAGC‑GGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGAc                                                                     <  1:1235446/102‑1 (MQ=255)

                                                                                              cccgcGATTATCGCTGCCTGGCAGC‑GGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGc                                                                                >  1:587504/1‑87 (MQ=255)

                                                                                              cccgcGATTATCGCTGCCTGGCAGC‑GGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGc                                                                                <  2:587504/87‑1 (MQ=255)

                                                                                              cccgcGATTATCGCTGCCTGGCAGC‑GGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAg                                               >  2:934625/1‑120 (MQ=255)

                                                                                              cccgcGATTATCGCTGCCTGGCAGC‑GGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAg                                               <  1:934625/120‑1 (MQ=255)

                                                                                                            tGCCAGGCAGCGGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTg                             >  1:814113/1‑125 (MQ=255)

                                                                                                            tGCCAGGCAGCGGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTg                             <  2:814113/125‑1 (MQ=255)

                                                                                                             gCCTGGCAGC‑GGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTgc             >  1:1164139/1‑139 (MQ=255)

                                                                                                                 ggCAGC‑GGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCaa         <  2:1164139/139‑1 (MQ=255)

                                                                                                                   cAGC‑GGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAg       >  2:1223831/1‑139 (MQ=255)

                                                                                                                         gggAGGTATTGATGGTGCATATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGAtttt  >  2:452642/1‑139 (MQ=255)

ACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGC‑GGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTT  >  NC_000913/385612‑385870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A