Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I1 R1	759	71.0	3673240	91.5%	3361014	104.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,148,691	G→A	357 (TAG→TAA)	plsX →	putative phosphate acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,148,691	0	G	A	100.0%	176.8 / NA	55	357 (TAG→TAA)	plsX	putative phosphate acyltransferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (23/32); total (23/32)

ATTGAACAGGCAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGA  >  NC_000913/1148569‑1148810

aTTGAACAGGCAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCg                                                                                                         >  1:1345390/1‑139 (MQ=255)

    aaCAGGCAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACt                                                                                                     <  1:536999/139‑1 (MQ=255)

      cAGGCAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCg                                                                                                   <  1:1151122/139‑1 (MQ=255)

      cAGGCAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCg                                                                                                   <  1:1551455/139‑1 (MQ=255)

             tGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTg                                                                                            >  2:1427361/1‑139 (MQ=255)

                 ggCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAg                                                                                        <  2:1070403/139‑1 (MQ=255)

                  gCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGt                                                                                       <  2:1384570/139‑1 (MQ=255)

                   cGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGTGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTg                                                                                      >  1:514550/1‑139 (MQ=255)

                   cGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTg                                                                                      >  1:1383372/1‑139 (MQ=255)

                    ggTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGTGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGa                                                                                     <  2:514550/139‑1 (MQ=255)

                    ggTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGa                                                                                     <  1:18779/139‑1 (MQ=255)

                       gCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACgg                                                                                  >  2:642897/1‑139 (MQ=255)

                           cGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGtata                                                                              <  1:1322370/139‑1 (MQ=255)

                             aCAAGTTCCTCAGCGAATTCCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGtatata                                                                            >  2:1223704/1‑139 (MQ=255)

                                           gAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACt                                                              <  2:604661/139‑1 (MQ=255)

                                             aTTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGa                                                            <  1:1427361/139‑1 (MQ=255)

                                                gcagCTCGCCTGCAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCg                                                         <  1:1213521/136‑1 (MQ=255)

                                                gCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCg                                                         <  2:940128/139‑1 (MQ=255)

                                                gCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCg                                                         <  2:1027961/139‑1 (MQ=255)

                                                    cTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTa                                                       >  2:1571702/1‑137 (MQ=255)

                                                    cTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTa                                                       <  1:1571702/137‑1 (MQ=255)

                                                    cTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACa                                                     <  2:1345390/139‑1 (MQ=255)

                                                             aaTCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGa                                            <  1:642897/139‑1 (MQ=255)

                                                                          aGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGttt                                                                                             >  1:509655/1‑77 (MQ=255)

                                                                          aGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGttt                                                                                             <  2:509655/77‑1 (MQ=255)

                                                                          aGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAAGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAATGTGACTGAGCGTACATGTATACGAAGATTATTGGTAc                               <  1:1223704/139‑1 (MQ=255)

                                                                           gCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAg                                                                                                           <  2:576792/62‑1 (MQ=255)

                                                                           gCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAg                                                                                                           >  1:576792/1‑62 (MQ=255)

                                                                           gCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACt                              >  2:540965/1‑139 (MQ=255)

                                                                           gCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACt                              >  2:1254081/1‑139 (MQ=255)

                                                                            cTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTa                                                       >  1:846659/1‑113 (MQ=255)

                                                                            cTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTa                                                       <  2:846659/113‑1 (MQ=255)

                                                                            cTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTg                             <  1:1378661/139‑1 (MQ=255)

                                                                            cTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTg                             >  2:1378661/1‑139 (MQ=255)

                                                                             tGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGttt                                                                                             >  1:82641/1‑74 (MQ=255)

                                                                             tGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGttt                                                                                             <  2:82641/74‑1 (MQ=255)

                                                                             tGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAg                                                                                        <  2:290500/79‑1 (MQ=255)

                                                                             tGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAg                                                                                        >  1:290500/1‑79 (MQ=255)

                                                                               gTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACg                                             >  2:600234/1‑120 (MQ=255)

                                                                               gTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACg                                             <  1:600234/120‑1 (MQ=255)

                                                                                      gctgctGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCg                                                                                                   >  2:1187241/1‑59 (MQ=255)

                                                                                      gctgctGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCg                                                                                                   <  1:1187241/59‑1 (MQ=255)

                                                                                             gACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGGGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGaa            <  1:743576/139‑1 (MQ=255)

                                                                                                  tGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGt       >  1:971709/1‑139 (MQ=255)

                                                                                                    gCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCg                                                                                                         >  2:1244832/1‑39 (MQ=38)

                                                                                                    gCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCg                                                                                                         <  1:1244832/39‑1 (MQ=38)

                                                                                                    gCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAg                                          <  2:1007076/102‑1 (MQ=255)

                                                                                                    gCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAg                                          >  1:1007076/1‑102 (MQ=255)

                                                                                                       aaaGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTCACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGa  <  2:971709/139‑1 (MQ=255)

                                                                                                             gAACTCTGCGGTAACAGGACGCTGCCAGCGAGCTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTAt                     <  1:1413084/114‑1 (MQ=255)

                                                                                                             gAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTAt                     >  2:1413084/1‑114 (MQ=255)

                                                                                                              aaCTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTa                                                       >  1:847634/1‑79 (MQ=255)

                                                                                                              aaCTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTa                                                       <  2:847634/79‑1 (MQ=255)

                                                                                                                     cGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTTCAAGTGa                                                                                     <  2:698473/42‑1 (MQ=255)

                                                                                                                     cGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTTCAAGTGa                                                                                     >  1:698473/1‑42 (MQ=255)

ATTGAACAGGCAGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGA  >  NC_000913/1148569‑1148810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A