Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I2 R1	765	53.2	2691310	93.4%	2513683	103.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,148,691	G→A	357 (TAG→TAA)	plsX →	putative phosphate acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,148,691	0	G	A	100.0%	105.1 / NA	34	357 (TAG→TAA)	plsX	putative phosphate acyltransferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/17); total (17/17)

AGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATT  >  NC_000913/1148580‑1148822

aGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGtt                                                                                                          >  2:281839/1‑139 (MQ=255)

aGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGtt                                                                                                          >  2:54554/1‑139 (MQ=255)

   gCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGc                                                                                                       >  1:1097059/1‑139 (MQ=255)

   gCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGc                                                                                                       >  1:1176161/1‑139 (MQ=255)

   gCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGc                                                                                                       >  2:854647/1‑139 (MQ=255)

      ggCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAg                                                                                                    <  2:472046/139‑1 (MQ=255)

         ggTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGa                                                                                                 <  1:1232451/139‑1 (MQ=255)

           tGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACg                                                                                               <  2:1176161/139‑1 (MQ=255)

              aGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGta                                                                                            <  2:472888/139‑1 (MQ=255)

                 gACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGtatat                                                                                         >  2:1151523/1‑139 (MQ=255)

                                gAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACt                                                                          <  1:183811/139‑1 (MQ=255)

                                gAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACt                                                                          <  1:281839/139‑1 (MQ=255)

                                 aaTTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCg                                                                                    >  2:271486/1‑128 (MQ=255)

                                 aaTTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCg                                                                                    <  1:271486/128‑1 (MQ=255)

                                     gCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCg                                                                     <  1:481151/139‑1 (MQ=255)

                                        gCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTAc                                                                  <  1:1151523/139‑1 (MQ=255)

                                                ggAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGCCACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTAGCAAGTGACGCTATATAACCGAAAAGTGACTGAGCGTACATGTATAc                                                          <  1:854647/139‑1 (MQ=255)

                                                               aGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCg                                                                     >  1:623469/1‑113 (MQ=255)

                                                               aGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCg                                                                     <  2:623469/113‑1 (MQ=255)

                                                                gCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGa                                                                            <  1:271527/105‑1 (MQ=255)

                                                                gCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGa                                                                            >  2:271527/1‑105 (MQ=255)

                                                                  tGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCg                                                                                                               >  2:995173/1‑68 (MQ=255)

                                                                  tGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCg                                                                                                               <  1:995173/68‑1 (MQ=255)

                                                                  tGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTgg                                        >  2:145781/1‑139 (MQ=255)

                                                                    gTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAg                                                                                                    <  2:1290956/77‑1 (MQ=255)

                                                                    gTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAg                                                                                                    >  1:1290956/1‑77 (MQ=255)

                                                                               ctgGACGGTGGCGAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCg                                                                                                               <  1:389212/55‑1 (MQ=255)

                                                                               ctgGACGGTGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCg                                                                                                               >  2:389212/1‑55 (MQ=255)

                                                                                       tGGCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGt                   >  1:590106/1‑139 (MQ=255)

                                                                                         gCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGta                                                                                            <  2:1013164/64‑1 (MQ=255)

                                                                                         gCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGta                                                                                            >  1:1013164/1‑64 (MQ=255)

                                                                                         gCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCg                                                                     <  1:968757/87‑1 (MQ=255)

                                                                                         gCAAAAGCGGAACTCTGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCg                                                                     >  2:968757/1‑87 (MQ=255)

                                                                                                        tGCGGTAACAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGAtt  >  2:427856/1‑139 (MQ=255)

AGTGCAGGCGGTGCAGCGACAAGTTCCTCAGCGAATTGCCGCTCGCCTGGAATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATT  >  NC_000913/1148580‑1148822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A