Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I1 R1	759	71.0	3673240	91.5%	3361014	104.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,934,874	T→C	T481A (ACC→GCC)	zwf ←	glucose‑6‑phosphate 1‑dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,934,874	0	T	C	100.0%	199.3 / NA	55	T481A (ACC→GCC)	zwf	glucose‑6‑phosphate 1‑dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (31/24); total (31/24)

CGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGA  >  NC_000913/1934738‑1935001

cGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCaa                                                                                                                               >  2:569004/1‑139 (MQ=255)

              cACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAg                                                                                                                 <  1:933641/139‑1 (MQ=255)

                   ggCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAAc                                                                                                            >  1:687773/1‑139 (MQ=255)

                    gCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACg                                                                                                           >  1:160361/1‑139 (MQ=255)

                       tGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGt                                                                                                        <  2:1300316/139‑1 (MQ=255)

                               tAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGt                                                                                                >  1:1824204/1‑139 (MQ=255)

                                   aaTTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCg                                                                                            <  1:84237/139‑1 (MQ=255)

                                     ttGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGc                                                                                          <  2:160361/139‑1 (MQ=255)

                                                    cgcAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTcc                                                                                             >  1:158499/1‑121 (MQ=255)

                                                    cgcAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTcc                                                                                             <  2:158499/121‑1 (MQ=255)

                                                    cgcAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCt                                                                                        <  1:1502018/126‑1 (MQ=255)

                                                    cgcAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCt                                                                                        >  2:1502018/1‑126 (MQ=255)

                                                    cgcAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCgg                                                                           >  1:1146316/1‑139 (MQ=255)

                                                      cAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGgcg                                                                         <  2:646769/139‑1 (MQ=255)

                                                              tGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTg                                                                 >  2:272704/1‑139 (MQ=255)

                                                                                cATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGCCCATCGCCCACGCCTCa                                               <  2:1146316/139‑1 (MQ=255)

                                                                                      ggATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATg                                         <  2:1824204/139‑1 (MQ=255)

                                                                                         tAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAg                                      >  2:1332075/1‑139 (MQ=255)

                                                                                          aaGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTc                                                                                              >  2:517916/1‑82 (MQ=255)

                                                                                          aaGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTc                                                                                              <  1:517916/82‑1 (MQ=255)

                                                                                                 gATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCa                              >  2:1406455/1‑139 (MQ=255)

                                                                                                  atatTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTc                                                                                              >  2:577262/1‑74 (MQ=255)

                                                                                                  atatTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTc                                                                                              <  1:577262/74‑1 (MQ=255)

                                                                                                       aCTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCa                        >  2:451858/1‑139 (MQ=255)

                                                                                                       aCTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCa                        >  2:1470139/1‑139 (MQ=255)

                                                                                                          cAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGc                     <  1:1406455/139‑1 (MQ=255)

                                                                                                            aaCTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGctt                   >  2:630952/1‑139 (MQ=255)

                                                                                                            aaCTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGctt                   >  2:1425256/1‑139 (MQ=255)

                                                                                                             aCTCATTCCAGGAACGACCATCACGGGCATTCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGcttc                  >  2:1158901/1‑139 (MQ=255)

                                                                                                             aCTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCgg                                                                                           >  1:1216456/1‑66 (MQ=255)

                                                                                                             aCTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCgg                                                                                           <  2:1216456/66‑1 (MQ=255)

                                                                                                              cTCATTCCAGGAACGACCATCACGGGCAAACATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGCTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGcttct                 >  2:1225575/1‑139 (MQ=255)

                                                                                                                  ttCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCAc                                                     <  2:308961/99‑1 (MQ=255)

                                                                                                                  ttCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCAc                                                     >  1:308961/1‑99 (MQ=255)

                                                                                                                   tCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCAc            >  1:764162/1‑139 (MQ=255)

                                                                                                                       ggAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGgcg                                                                         >  1:1492548/1‑74 (MQ=255)

                                                                                                                       ggAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGgcg                                                                         <  2:1492548/74‑1 (MQ=255)

                                                                                                                           cGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTcgc    >  2:1320818/1‑139 (MQ=255)

                                                                                                                           cGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTcgc    <  2:687773/139‑1 (MQ=255)

                                                                                                                           cGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTcgc    <  1:1320818/139‑1 (MQ=255)

                                                                                                                             aCCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGc                                                                                          <  2:1814312/51‑1 (MQ=255)

                                                                                                                             aCCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGc                                                                                          >  1:1814312/1‑51 (MQ=255)

                                                                                                                             aCCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCa                                                             <  1:973395/80‑1 (MQ=255)

                                                                                                                             aCCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCa                                                             >  2:973395/1‑80 (MQ=255)

                                                                                                                             aCCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGcc                                                        >  2:1778887/1‑85 (MQ=255)

                                                                                                                             aCCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGcc                                                        <  1:1778887/85‑1 (MQ=255)

                                                                                                                             aCCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGcc                                                        >  1:1366979/1‑85 (MQ=255)

                                                                                                                             aCCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGcc                                                        <  2:1366979/85‑1 (MQ=255)

                                                                                                                             aCCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGcc                                                        <  2:1224922/85‑1 (MQ=255)

                                                                                                                             aCCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGa  <  1:630952/139‑1 (MQ=255)

                                                                                                                             aCCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGa  >  1:857170/1‑139 (MQ=255)

                                                                                                                             aCCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGa  >  1:1468174/1‑139 (MQ=255)

                                                                                                                             aCCATCACGGGCAATAATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGcc                                                        >  1:1224922/1‑85 (MQ=255)

                                                                                                                                 tCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGt                                                                >  1:276550/1‑73 (MQ=255)

                                                                                                                                 tCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGt                                                                <  2:276550/73‑1 (MQ=255)

CGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGA  >  NC_000913/1934738‑1935001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A