Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A9 F1 I2 R1 | 760 | 52.7 | 2744488 | 90.2% | 2475528 | 105.0 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,934,874 | T→C | T481A (ACC→GCC) | zwf ← | glucose‑6‑phosphate 1‑dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,934,874 | 0 | T | C | 100.0% | 153.6 / NA | 44 | T481A (ACC→GCC) | zwf | glucose‑6‑phosphate 1‑dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/22); total (22/22) | |||||||||||
CGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGC > NC_000913/1934738‑1934999 | cGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCaa > 2:43387/1‑139 (MQ=255) aTCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCa < 2:884130/139‑1 (MQ=255) aGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCaa > 2:1001423/1‑139 (MQ=255) ccTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACg < 2:1231600/138‑1 (MQ=255) ccTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACg > 1:1231600/1‑138 (MQ=255) tAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGt > 1:889175/1‑139 (MQ=255) aaTTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCg < 1:43387/139‑1 (MQ=255) gTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGc < 1:1021352/116‑1 (MQ=255) gTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGc > 2:1021352/1‑116 (MQ=255) cgcAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCgg < 1:130779/139‑1 (MQ=255) ccGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCg > 1:1099545/1‑107 (MQ=255) ccGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCg < 2:1099545/107‑1 (MQ=255) tAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGcc > 2:1184211/1‑139 (MQ=255) aaTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCAc > 2:464610/1‑139 (MQ=255) aaGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTaa < 2:889175/139‑1 (MQ=255) tAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCa > 2:1168084/1‑123 (MQ=255) tAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCa < 1:1168084/123‑1 (MQ=255) aaGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCgg > 2:752248/1‑101 (MQ=255) aaGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCgg < 1:752248/101‑1 (MQ=255) aaGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGgcg > 2:434081/1‑103 (MQ=255) aaGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGgcg < 1:434081/103‑1 (MQ=255) gcAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTAc < 1:1184211/139‑1 (MQ=255) ttACTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTc < 1:1001423/139‑1 (MQ=255) aCTCAAACTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCa > 2:497504/1‑139 (MQ=255) aaCTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGgcg > 2:15076/1‑85 (MQ=255) aaCTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGgcg < 1:15076/85‑1 (MQ=255) aaCTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGAGCATCCGTGTCCATCGCCCCCGCCTCAGTAATGGAGCTTACCCATTTCCAGGctt > 1:1335852/1‑139 (MQ=255) aCTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTcccc > 1:217090/1‑57 (MQ=255) aCTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTcccc < 2:217090/57‑1 (MQ=255) aCTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGgcg > 2:763814/1‑84 (MQ=255) aCTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGgcg < 1:763814/84‑1 (MQ=255) aCTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCt > 1:125656/1‑108 (MQ=255) aCTCATTCCAGGAACGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCt < 2:125656/108‑1 (MQ=255) ttCCAGGAACGACCATCACGGGCAATCATCGCCAcc < 1:93992/36‑1 (MQ=38) ttCCAGGAACGACCATCACGGGCAATCATCGCCAcc > 2:93992/1‑36 (MQ=255) aaCGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGcc < 1:16440/89‑1 (MQ=255) aaCGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGcc > 2:16440/1‑89 (MQ=255) cGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAAt > 2:1167437/1‑101 (MQ=255) cGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAAt < 1:1167437/101‑1 (MQ=255) cGACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTcgc < 1:497504/139‑1 (MQ=255) gACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGgcg > 2:1320722/1‑69 (MQ=255) gACCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGgcg < 1:1320722/69‑1 (MQ=255) aCCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGcc > 1:1131646/1‑52 (MQ=255) aCCATCACGGGCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGcc < 2:1131646/52‑1 (MQ=255) | CGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGC > NC_000913/1934738‑1934999 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |