Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I1 R1	759	71.0	3673240	91.5%	3361014	104.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,049,336	C→T	P1467S (CCG→TCG)	yeeJ →	putative adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,049,336	0	C	T	100.0%	167.6 / NA	52	P1467S (CCG→TCG)	yeeJ	putative adhesin
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (20/32); total (20/32)

TTTACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATCCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTACTCTGCCGGAAGATGTGAAGGCGAACTTCACGC  >  NC_000913/2049207‑2049462

tttACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTg                                                                                                                       <  1:472156/139‑1 (MQ=255)

 ttACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGc                                                                                                                      <  1:1047932/139‑1 (MQ=255)

  tACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTAATTGcc                                                                                                                     <  2:99245/139‑1 (MQ=255)

   aCCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCg                                                                                                                    >  1:537692/1‑139 (MQ=255)

      gCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACa                                                                                                                 <  2:1474849/139‑1 (MQ=255)

        tACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGcc                                                                                                                     >  1:196104/1‑133 (MQ=255)

        tACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGcc                                                                                                                     <  2:196104/133‑1 (MQ=255)

        tACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAAt                                                                                                               >  1:1316933/1‑139 (MQ=255)

        tACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAAt                                                                                                               >  2:954396/1‑139 (MQ=255)

         aCAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGcc                                                                                                                     >  2:169024/1‑132 (MQ=255)

         aCAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGcc                                                                                                                     <  1:169024/132‑1 (MQ=255)

              aGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGAt                                                                                                         <  2:465062/139‑1 (MQ=255)

                 cGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGAtctc                                                                                                      <  1:954396/139‑1 (MQ=255)

                    ggTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGa                                                                                                                   >  2:493635/1‑123 (MQ=255)

                    ggTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGa                                                                                                                   <  1:493635/123‑1 (MQ=255)

                         aTCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACt                                                                                              >  1:970346/1‑139 (MQ=255)

                                   ggCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACa                                                                                    <  2:987031/139‑1 (MQ=255)

                                   ggCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCACGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACa                                                                                    <  1:1442827/139‑1 (MQ=255)

                                     cAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGt                                                                                  <  2:367847/139‑1 (MQ=255)

                                     cAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGt                                                                                  <  1:1486816/139‑1 (MQ=255)

                                     cAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGt                                                                                  <  1:1149359/139‑1 (MQ=255)

                                          cTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTg                                                                             <  2:1316933/139‑1 (MQ=255)

                                                aaTGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACag                                                                       <  1:1446213/139‑1 (MQ=255)

                                                   ggCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGAtctc                                                                                                      <  1:1761116/105‑1 (MQ=255)

                                                   ggCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGAtctc                                                                                                      >  2:1761116/1‑105 (MQ=255)

                                                              gCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATa                                                         <  2:1465266/139‑1 (MQ=255)

                                                                     acaGTGACCTATGTGCCGAACGTCGCTAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAaca                                                  <  1:1168206/139‑1 (MQ=255)

                                                                          gACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAg                                             <  2:1678612/139‑1 (MQ=255)

                                                                           aCCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGa                                                                                                                   <  1:1505303/68‑1 (MQ=255)

                                                                           aCCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGa                                                                                                                   >  2:1505303/1‑68 (MQ=255)

                                                                            ccTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGt                                           <  2:537692/139‑1 (MQ=255)

                                                                                tgtgCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGt                                                                                  >  1:788482/1‑96 (MQ=255)

                                                                                tgtgCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGt                                                                                  <  2:788482/96‑1 (MQ=255)

                                                                                 gtgCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGACAATGCGATAGCCAACACTGAGGTAACAt                                      >  1:167340/1‑139 (MQ=255)

                                                                                     cGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGcc                                                                                                                     >  2:1395865/1‑56 (MQ=255)

                                                                                     cGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGcc                                                                                                                     <  1:1395865/56‑1 (MQ=255)

                                                                                      gAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTAct                                 <  2:970346/139‑1 (MQ=255)

                                                                                            cgcgAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATaa                                                                                                             <  2:838897/57‑1 (MQ=255)

                                                                                            cgcgAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATaa                                                                                                             >  1:838897/1‑57 (MQ=255)

                                                                                            cgcgAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGAtct                                                                                                       >  1:254321/1‑63 (MQ=255)

                                                                                            cgcgAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGAtct                                                                                                       <  2:254321/63‑1 (MQ=255)

                                                                                             gcgAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTACTCTGCCgg                          >  1:102707/1‑139 (MQ=255)

                                                                                              cgAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTACTCTGcc                            >  1:638618/1‑136 (MQ=255)

                                                                                              cgAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTACTCTGcc                            <  2:638618/136‑1 (MQ=255)

                                                                                                   gCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTACTCTGCCGGAAGAtg                    <  1:699781/139‑1 (MQ=255)

                                                                                                       aaaTCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGAtctc                                                                                                      >  1:1056649/1‑53 (MQ=255)

                                                                                                       aaaTCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGAtctc                                                                                                      <  2:1056649/53‑1 (MQ=255)

                                                                                                        aaTCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTACTCTGCCGGAAGATGTGAAg               >  1:1679731/1‑139 (MQ=255)

                                                                                                                 ggCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACa                                                                                    >  1:1690029/1‑61 (MQ=255)

                                                                                                                 ggCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACa                                                                                    <  2:1690029/61‑1 (MQ=255)

                                                                                                                  gCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTACTCTGCCGGAAGATGTGAAGGCGAACTTCa     <  2:102707/139‑1 (MQ=255)

                                                                                                                     gCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTACTCTGCCGGAAGATGTGAAGGCGAACTTCACGc  >  1:1645569/1‑139 (MQ=255)

TTTACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATCCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTACTCTGCCGGAAGATGTGAAGGCGAACTTCACGC  >  NC_000913/2049207‑2049462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A