Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,049,336	C→T	P1467S (CCG→TCG)	yeeJ →	putative adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,049,336	0	C	T	100.0%	175.8 / NA	55	P1467S (CCG→TCG)	yeeJ	putative adhesin
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (18/37); total (18/37)

TTACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATCCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTACT  >  NC_000913/2049208‑2049431

ttACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGc                                                                                       <  1:916444/139‑1 (MQ=255)

 tACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGcc                                                                                      <  2:585031/139‑1 (MQ=255)

  aCCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCg                                                                                     >  1:194524/1‑139 (MQ=255)

    cGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGAc                                                                                   <  1:185999/139‑1 (MQ=255)

     gCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACa                                                                                  >  2:380229/1‑139 (MQ=255)

       tACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACa                                                                                  >  1:38870/1‑137 (MQ=255)

       tACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACa                                                                                  <  2:38870/137‑1 (MQ=255)

       tACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAAt                                                                                >  1:196141/1‑139 (MQ=255)

       tACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAAt                                                                                <  1:732084/139‑1 (MQ=255)

        aCAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATa                                                                               >  2:988024/1‑139 (MQ=255)

                cGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGAtctc                                                                       <  1:135182/139‑1 (MQ=255)

                   ggTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACg                                                                    <  2:545292/139‑1 (MQ=255)

                      ttATCAATTGACGGCAACCCTCGAAAATGGCGAATCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGaca                                                                 <  1:552505/139‑1 (MQ=255)

                        aTCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACt                                                               <  2:194524/139‑1 (MQ=255)

                            aTTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACa                                                           >  1:725520/1‑139 (MQ=255)

                             ttGACGGCAACCATCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAg                                                          >  2:713336/1‑139 (MQ=255)

                              tGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGc                                                         >  2:653402/1‑139 (MQ=255)

                               gACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCa                                                        >  1:786015/1‑139 (MQ=255)

                                  ggCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACa                                                     <  1:600067/139‑1 (MQ=255)

                                  ggCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACa                                                     <  2:386349/139‑1 (MQ=255)

                                  ggCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACa                                                     <  1:386039/139‑1 (MQ=255)

                                  ggCAACCATCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACa                                                     <  1:713336/139‑1 (MQ=255)

                                    cAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGt                                                   <  2:50757/139‑1 (MQ=255)

                                    cAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGt                                                   <  2:725520/139‑1 (MQ=255)

                                    cAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGt                                                   <  1:830896/139‑1 (MQ=255)

                                    cAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGt                                                   <  1:380229/139‑1 (MQ=255)

                                      aCCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCg                                                 <  2:107995/139‑1 (MQ=255)

                                      aCCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCg                                                 <  2:811692/139‑1 (MQ=255)

                                         cTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTg                                              <  1:988024/139‑1 (MQ=255)

                                          tCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGa                                             >  2:616490/1‑139 (MQ=255)

                                                aTGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACaga                                       <  1:225030/139‑1 (MQ=255)

                                                     gATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCa                                  <  1:793253/139‑1 (MQ=255)

                                                               aacaaacaGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGc                        <  1:743638/139‑1 (MQ=255)

                                                               aacaaacaGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGc                        <  1:253137/139‑1 (MQ=255)

                                                                acaaacaGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGcc                       <  2:205898/139‑1 (MQ=255)

                                                                     caGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAacac                  <  2:483466/139‑1 (MQ=255)

                                                                     caGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAacac                  <  2:474043/139‑1 (MQ=255)

                                                                        tGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGa               >  1:14345/1‑139 (MQ=255)

                                                                        tGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGa               >  2:434834/1‑139 (MQ=255)

                                                                         gACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGc                                                >  1:125384/1‑105 (MQ=255)

                                                                         gACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGc                                                <  2:125384/105‑1 (MQ=255)

                                                                         gACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGa                            >  2:428536/1‑125 (MQ=255)

                                                                         gACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGa                            <  1:428536/125‑1 (MQ=255)

                                                                         gACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAg              <  1:616490/139‑1 (MQ=255)

                                                                         gACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAg              >  2:228013/1‑139 (MQ=255)

                                                                         gACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAg              <  2:744573/139‑1 (MQ=255)

                                                                           ccTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGt            <  2:197460/139‑1 (MQ=255)

                                                                                    cGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACa                                                     >  2:31678/1‑89 (MQ=255)

                                                                                    cGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACa                                                     <  1:31678/89‑1 (MQ=255)

                                                                                     gAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTAct  <  1:128605/139‑1 (MQ=255)

                                                                                     gAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTAct  <  1:228013/139‑1 (MQ=255)

                                                                                      aaCGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCaa                                                       <  2:625480/85‑1 (MQ=255)

                                                                                      aaCGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCaa                                                       >  1:625480/1‑85 (MQ=255)

                                                                                                             gCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATa                                                                               <  1:923162/38‑1 (MQ=255)

                                                                                                             gCTGGCAGCCTCGAAGGATTCGGTGATTGCCGACAATa                                                                               >  2:923162/1‑38 (MQ=255)

TTACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAACCCTCGAAAATGGCGATTCGATGCAACAAACAGTGACCTATGTGCCGAACGTCGCGAATGCTGAAATCACGCTGGCAGCCTCGAAGGATCCGGTGATTGCCGACAATAACGATCTCACGACACTAACAGCAACAGTCGCTGATACAGAGGGCAATGCGATAGCCAACACTGAGGTAACATTTACT  >  NC_000913/2049208‑2049431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A