Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I1 R1	759	71.0	3673240	91.5%	3361014	104.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,199,998	A→G	pseudogene (677/948 nt)	yehH →	DUF4132 family pseudogene, defective yehI paralog, molybdate metabolism regulator, first fragment, molybdate metabolism regulator, second fragment 2, yehI' molR mutations have phenotypes and the N‑terminal molR fragment of yehI' may be expressed and may have retained or evolved MolR function

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,199,998	0	A	G	100.0%	190.4 / NA	53	pseudogene (677/948 nt)	yehH	DUF4132 family pseudogene, defective yehI paralog, molybdate metabolism regulator, first fragment, molybdate metabolism regulator, second fragment 2, yehI' molR mutations have phenotypes and the N‑terminal molR fragment of yehI' may be expressed and may have retained or evolved MolR function
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (25/28); total (25/28)

CACCGTTCCGCCAGCTCGACCGTAACAGCTACGCCCTGACAGAAGCCGAGCGCAATGCCAGTGAACTGACCCGCTGGGCAGGCAGAAAATGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAAGGGCGAACCACAGGATGGAGGCTGGATCGGATGGATGATCAAACCTTTGGGTCGCTGGTCGTTAATCATGGAAATCGATGAAGGCTTTGCGGTAGGCATGTCGCCAGCCGAACTCAGCGCTGAGC  >  NC_000913/2199863‑2200123

caccGTTCCGCCAGCTCGACCGTAACAGCTACGCCCTGACAGAAGCCGAGCGCAATGCCAGTGAACTGACCCGCTGGGCAGGCAGAAAATGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAgggg                                                                                                                            <  2:435127/139‑1 (MQ=255)

             gCTCGACCGTAACAGCTACGCCCTGACAGAAGCCGAGCGCAATGCCAGTGAACTGACCCGCTGGGCAGGCAGAAAATGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCGAACCACAGGAt                                                                                                               <  2:573120/139‑1 (MQ=255)

                       aaCAGCTACGCCCTGACAGAAGCCGAGCGCAATGCCAGTGAACTGACCCGCTGGGCAGGCAGAAAATGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCGAACCACAGGATGGAGGCTGGa                                                                                                     <  2:12858/139‑1 (MQ=255)

                        aCAGCTACGCCCTGACAGAAGCCGAGCGCAATGCCAGTGAACTGACCCGCTGGGCAGGCAGAAAATGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCGAACCACAGGATGGAGGCTGGAt                                                                                                    <  2:986547/139‑1 (MQ=255)

                             tACGCCCTGACAGAAGCCGAGCGCAATGCCAGTGAACTGACCCGCTGGGCAGGCAGAAAATGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCGAACCACAGGATg                                                                                                              >  1:260312/1‑124 (MQ=255)

                             tACGCCCTGACAGAAGCCGAGCGCAATGCCAGTGAACTGACCCGCTGGGCAGGCAGAAAATGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCGAACCACAGGATg                                                                                                              <  2:260312/124‑1 (MQ=255)

                              aCGCCCTGACAGAAGCCGAGCGCAATGCCAGTGAACTGACCCGCTGGGCAGGCAGAAAATGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCg                                                                                                                          >  1:1680906/1‑111 (MQ=255)

                              aCGCCCTGACAGAAGCCGAGCGCAATGCCAGTGAACTGACCCGCTGGGCAGGCAGAAAATGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCg                                                                                                                          <  2:1680906/111‑1 (MQ=255)

                              aCGCCCTGACAGAAGCCGAGCGCAATGCCAGTGAACTGACCCGCTGGGCAGGCAGAAAATGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCGAACCACAGGATGGAGGCTGGATCggatg                                                                                              >  2:1528160/1‑139 (MQ=255)

                              aCGCCCTGACAGAAGCCGAGCGCAATGCCAGTGAACTGACCCGCTGGGCAGGCAGAAAATGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCGAACCACAGGATGGAGGCTGGATCggatg                                                                                              <  2:86613/139‑1 (MQ=255)

                               cGCCCTGACAGAAGCCGAGCGCAATGCCAGTGAACTGACCCGCTGGGCAGGCAGAAAATGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCGAACCACAGGATGGAGGCTGGATCggatgg                                                                                             <  2:1017457/139‑1 (MQ=255)

                                 cccTGACAGAAGCCGAGCGCAATGCCAGTGAACTGACCCGCTGGGCAGGCAGAAAATGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCGAACCACAGGATGGa                                                                                                            >  1:195880/1‑122 (MQ=255)

                                 cccTGACAGAAGCCGAGCGCAATGCCAGTGAACTGACCCGCTGGGCAGGCAGAAAATGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCGAACCACAGGATGGa                                                                                                            <  2:195880/122‑1 (MQ=255)

                                      aCAGAAGCCGAGCGCAATGCCAGTGAACTGACCCGCTGGGCAGGCAGAAAATGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCGAACca                                                                                                                     >  1:1512913/1‑108 (MQ=255)

                                      aCAGAAGCCGAGCGCAATGCCAGTGAACTGACCCGCTGGGCAGGCAGAAAATGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCGAACca                                                                                                                     <  2:1512913/108‑1 (MQ=255)

                                      aCAGAAGCCGAGCGCAATGCCAGTGAACTGACCCGCTGGGCAGGCAGAAAATGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCGAACca                                                                                                                     >  1:86030/1‑108 (MQ=255)

                                      aCAGAAGCCGAGCGCAATGCCAGTGAACTGACCCGCTGGGCAGGCAGAAAATGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCGAACca                                                                                                                     <  2:86030/108‑1 (MQ=255)

                                          aaGCCGAGCGCAATGCCAGTGAACTGACCCGCTGGGCAGGCAGAAAATGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCG‑ACCACAGGATGGAGGCTGGATCGGATGGATGATCAAACCt                                                                                 >  2:1196422/1‑139 (MQ=255)

                                              cGAGCGCAATGCCAGTGAACTGACCCGCTGGGCAGGCAGAAAATGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCg                                                                                                                          >  2:133115/1‑95 (MQ=255)

                                              cGAGCGCAATGCCAGTGAACTGACCCGCTGGGCAGGCAGAAAATGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCg                                                                                                                          <  1:133115/95‑1 (MQ=255)

                                              cGAGCGCAATGCCAGTGAACTGACCCGCTGGGCAGGCAGAAAATGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCGAACCACAGGAt                                                                                                               <  1:1359281/106‑1 (MQ=255)

                                              cGAGCGCAATGCCAGTGAACTGACCCGCTGGGCAGGCAGAAAATGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCGAACCACAGGAt                                                                                                               >  2:1359281/1‑106 (MQ=255)

                                                  cgcAATGCCAGTGAACTGACCCGCTGGGCAGGCAGAAAATGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCGAACCACAGGATGGAGGCTGGATCGGATGGATGATCAAACCTTTGGGTc                                                                          >  1:1335099/1‑139 (MQ=255)

                                                     aaTGCCAGTGAACTGACCCGCTGGGCAGGCAGAAAATGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCGAACCACAGGATGGAGGCTGGATCGGATGGATGATCAAACCTTTGGGTCGCt                                                                       <  2:1003309/139‑1 (MQ=255)

                                                            gTGAACTGACCCGCTGGGCAGGCAGAAAATGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCGAACCACAGGATGGAGGCTGGATCGGATGGATGATCAAACCTTTGGGTCGCTGGTCGtt                                                                >  1:164427/1‑139 (MQ=255)

                                                                   gACCCGCTGGGCAGGCAGAAAATGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCGAACCACAGGATGGAGGCTGGATCGGATGGATGATCAAACCTTTGGGTCGCTGGTCGTTAATCATg                                                         >  1:1434032/1‑139 (MQ=255)

                                                                    aCCCGCTGGGCAGGCAGAAAATGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCGAACCACAGGATGGAGGCTGGa                                                                                                     >  1:1598576/1‑94 (MQ=255)

                                                                    aCCCGCTGGGCAGGCAGAAAATGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCGAACCACAGGATGGAGGCTGGa                                                                                                     <  2:1598576/94‑1 (MQ=255)

                                                                         cTGGGCAGGCAGAAAATGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCGAACCACAGGATGGAGGCTGGATCGGATGGATGATCAAACCt                                                                                 >  2:601486/1‑109 (MQ=255)

                                                                         cTGGGCAGGCAGAAAATGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCGAACCACAGGATGGAGGCTGGATCGGATGGATGATCAAACCt                                                                                 <  1:601486/109‑1 (MQ=255)

                                                                         cTGGGCAGGCAGAAAATGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCGAACCACAGGATGGAGGCTGGATCGGATGGATGATCAAACCTTTGGGTCGCTGGTCGTTaa                                                              >  2:86159/1‑128 (MQ=255)

                                                                         cTGGGCAGGCAGAAAATGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCGAACCACAGGATGGAGGCTGGATCGGATGGATGATCAAACCTTTGGGTCGCTGGTCGTTaa                                                              <  1:86159/128‑1 (MQ=255)

                                                                         cTGGGCAGGCAGAAAATGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCGAACCACAGGATGGAGGCTGGATCGGATGGATGATCAAACCTTTGGGTCGCTGGTCGTTAATCATGGa                                                       >  2:1332841/1‑135 (MQ=255)

                                                                         cTGGGCAGGCAGAAAATGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCGAACCACAGGATGGAGGCTGGATCGGATGGATGATCAAACCTTTGGGTCGCTGGTCGTTAATCATGGa                                                       <  1:1332841/135‑1 (MQ=255)

                                                                                      aaaTGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCGAACCACAGGATGGAGGCTGGATCGGATGGATGATCAAACCTTTGGGTCGCTGGTCGTTAATCATGGAAATCGATGAAGGCTTTg                                      <  2:1335099/139‑1 (MQ=255)

                                                                                           cccGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCGAACCACAGGATGGAGGCTGGATCGGATGGATGATCAAACCTTTggg                                                                            <  1:342746/96‑1 (MQ=255)

                                                                                           cccGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCGAACCACAGGATGGAGGCTGGATCGGATGGATGATCAAACCTTTggg                                                                            >  2:342746/1‑96 (MQ=255)

                                                                                               aGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCGAACCACAGGATGGAGGCTGGATCggatgg                                                                                             <  2:872823/75‑1 (MQ=255)

                                                                                               aGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAGGGGCGAACCACAGGATGGAGGCTGGATCggatgg                                                                                             >  1:872823/1‑75 (MQ=255)

                                                                                                             ggggCTGGCGAATAAAGGCTGGATAAGGGGCGAACCACAGGATGGAgg                                                                                                          >  1:1068677/1‑48 (MQ=255)

                                                                                                             ggggCTGGCGAATAAAGGCTGGATAAGGGGCGAACCACAGGATGGAgg                                                                                                          <  2:1068677/48‑1 (MQ=255)

                                                                                                                  tGGCGAATAAAGGCTGGATAAGGGGCGAACCACAGGATGGAGGCTGGAt                                                                                                    >  1:1814135/1‑49 (MQ=255)

                                                                                                                  tGGCGAATAAAGGCTGGATAAGGGGCGAACCACAGGATGGAGGCTGGAt                                                                                                    <  2:1814135/49‑1 (MQ=255)

                                                                                                                  tGGCGAATAAAGGCTGGATAAGGGGCGAACCACAGGATGGAGGCTGGATCgga                                                                                                >  2:1159139/1‑53 (MQ=255)

                                                                                                                  tGGCGAATAAAGGCTGGATAAGGGGCGAACCACAGGATGGAGGCTGGATCgga                                                                                                <  1:1159139/53‑1 (MQ=255)

                                                                                                                  tGGCGAATAAAGGCTGGATAAGGGGCGAACCACAGGATGGAGGCTGGATCGGATGGATGATCAAACCTTTGGGTCGCTGGTCGTTAATCATGGAAATCGATGAAGGCTTTGCGGTAGGCATGTCGCCAGCCGAACTCAg          >  2:864121/1‑139 (MQ=255)

                                                                                                                    gCGAATAAAGGCTGGATAAGGGGCGAACCACAGGATGGAGGCTGGATCGGATGGATGATCAAACCTTTGGGTCGCTGGTCGTTAATCATGGAAATCGATGAAGGCTTTGCGGTAGGCATGTCGCCAGCCGAACTCAgcg        <  1:1528160/139‑1 (MQ=255)

                                                                                                                      gAATAAAGGCTGGATAAGGGGCGAACCACAGGATGGAgg                                                                                                          >  2:1177095/1‑39 (MQ=255)

                                                                                                                      gAATAAAGGCTGGATAAGGGGCGAACCACAGGATGGAgg                                                                                                          <  1:1177095/39‑1 (MQ=255)

                                                                                                                          aaaGGCTGGATAAGGGGCGAACCACAGGATGGAGGCTGGATCGGATGGATGATCAAACCTTTGGGTCGCTGGTCGTTAATCATGGAAATCGATGAAGGCTTTGCGGTAGGCATGTCGCCAGCCGAACTCAGCGCTGagc  <  1:864121/139‑1 (MQ=255)

                                                                                                                                  gATAAGGGGCGAACCACAGGATGGAGGCTGGATCGGATGGATGATCAAACCTTTGGGTCGCTGGTCGTTa                                                               <  2:677546/70‑1 (MQ=255)

                                                                                                                                  gATAAGGGGCGAACCACAGGATGGAGGCTGGATCGGATGGATGATCAAACCTTTGGGTCGCTGGTCGTTa                                                               >  1:677546/1‑70 (MQ=255)

                                                                                                                                  gATAAGGGGCGAACCACAGGATGGAGGCTGGATCGGATGGATGATCAAACCTTTGGGTCGCTGGTCGTTAATCATGGAAATCGATGAAGGCTTTGc                                     <  1:96378/96‑1 (MQ=255)

                                                                                                                                  gATAAGGGGCGAACCACAGGATGGAGGCTGGATCGGATGGATGATCAAACCTTTGGGTCGCTGGTCGTTAATCATGGAAATCGATGAAGGCTTTGc                                     >  2:96378/1‑96 (MQ=255)

CACCGTTCCGCCAGCTCGACCGTAACAGCTACGCCCTGACAGAAGCCGAGCGCAATGCCAGTGAACTGACCCGCTGGGCAGGCAGAAAATGCCCGAGTGGTCGGGTCATGGGGCTGGCGAATAAAGGCTGGATAAAGGGCGAACCACAGGATGGAGGCTGGATCGGATGGATGATCAAACCTTTGGGTCGCTGGTCGTTAATCATGGAAATCGATGAAGGCTTTGCGGTAGGCATGTCGCCAGCCGAACTCAGCGCTGAGC  >  NC_000913/2199863‑2200123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A