Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F1 I1 R1 759 71.0 3673240 91.5% 3361014 104.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,297,114 (C)7→8 coding (531/624 nt) ccmA ← heme export ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,297,1071.C100.0% 180.9 / NA 51I180V (ATT→GTT) ccmAheme export ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (26/25);  total (26/25)

ATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAAT‑CCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGACGCGCTAAAGCGACGCGG  >  NC_000913/2296974‑2297240
                                                                                                                                      |                                                                                                                                     
aTGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAAT‑ccccc                                                                                                                                  <  1:776933/139‑1 (MQ=255)
  ggCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAAT‑ccccccc                                                                                                                                <  1:189487/139‑1 (MQ=255)
  ggCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAAT‑ccccccc                                                                                                                                <  2:1625988/139‑1 (MQ=255)
    cGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAAT‑cccccccct                                                                                                                              <  1:614655/139‑3 (MQ=255)
         cGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTcc                                                                                                                          >  1:1205033/1‑139 (MQ=255)
         cGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTcc                                                                                                                          >  1:864030/1‑139 (MQ=255)
         cGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAAT‑cccccccctgc                                                                                                                            <  1:606300/136‑5 (MQ=255)
         cGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAAT‑cccccccctgc                                                                                                                            >  2:606300/1‑132 (MQ=255)
              cacGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTAtg                                                                                                                     <  2:1205033/139‑1 (MQ=255)
              cacGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTAtg                                                                                                                     <  1:1364003/139‑1 (MQ=255)
                    gCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAAT‑cc                                                                                                                                     <  1:1700434/116‑1 (MQ=255)
                    gCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAAT‑cc                                                                                                                                     >  2:1700434/1‑116 (MQ=255)
                      tCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCa                                                                                                             >  1:878064/1‑139 (MQ=255)
                          gACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACg                                                                                                         >  1:753755/1‑139 (MQ=255)
                               aaaaTGCGCCAGAACTTCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTggg                                                                                                    >  1:1225273/1‑139 (MQ=255)
                                 aaTGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTc                                                                                                  <  2:864030/139‑1 (MQ=255)
                                   tGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAg                                                                                                <  2:878064/139‑1 (MQ=255)
                                    gcgcCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGa                                                                                               <  2:180546/139‑1 (MQ=255)
                                      gcCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACg                                                                                             >  2:49084/1‑139 (MQ=255)
                                         aGAACATCATGCGGCCCTCGTTTGCGTCAATGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATc                                                                                          >  2:205516/1‑139 (MQ=255)
                                            aCATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGac                                                                                       <  2:1225273/139‑1 (MQ=255)
                                             catcatGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGaca                                                                                      >  1:380270/1‑139 (MQ=255)
                                                catGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATAc                                                                                                          <  2:1264131/116‑1 (MQ=255)
                                                catGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATAc                                                                                                          >  1:1264131/1‑116 (MQ=255)
                                                    cGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTaa                                                                               >  2:253297/1‑139 (MQ=255)
                                                        ccTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTc                                                                           >  2:1286977/1‑139 (MQ=255)
                                                                 cGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGt                                                                  >  1:1829981/1‑139 (MQ=255)
                                                                            tGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCtcgt                                                       <  2:1362078/139‑1 (MQ=255)
                                                                            tGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCtcgt                                                       >  2:445274/1‑139 (MQ=255)
                                                                            tGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCtcgt                                                       <  1:205516/139‑1 (MQ=255)
                                                                              cggcgAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCtcgtcg                                                     <  2:753755/139‑1 (MQ=255)
                                                                                gcgAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAg                                                   <  1:826032/139‑1 (MQ=255)
                                                                                 cgAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGTTCGTCGAgg                                                  >  1:716204/1‑139 (MQ=255)
                                                                                             tCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTg                                      >  2:154453/1‑139 (MQ=255)
                                                                                                      ttGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTc                             <  1:154453/139‑1 (MQ=255)
                                                                                                         aGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCtcgt                                                       <  2:425864/110‑1 (MQ=255)
                                                                                                         aGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCtcgt                                                       >  1:425864/1‑110 (MQ=255)
                                                                                                              cTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCtcgtcg                                                     <  2:34613/107‑1 (MQ=255)
                                                                                                              cTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCtcgtcg                                                     >  1:34613/1‑107 (MQ=255)
                                                                                                              cTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAg                                                   <  1:1422217/109‑1 (MQ=255)
                                                                                                              cTGGTGGGTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAg                                                   >  2:1422217/1‑109 (MQ=255)
                                                                                                                   gggTAGTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGAcgcg                >  2:1771601/1‑139 (MQ=255)
                                                                                                                        gTCAGAGTCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGtt                                                                                 <  2:1090452/69‑1 (MQ=255)
                                                                                                                        gTCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGtt                                                                                 >  1:1090452/1‑69 (MQ=255)
                                                                                                                         tCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTg                                                                                                      <  2:990963/47‑1 (MQ=39)
                                                                                                                         tCAGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTg                                                                                                      >  1:990963/1‑47 (MQ=39)
                                                                                                                           aGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGcc                                                                                                              <  2:1545517/37‑1 (MQ=255)
                                                                                                                           aGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGcc                                                                                                              >  1:1545517/1‑37 (MQ=255)
                                                                                                                           aGAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGACGCGCTAAAGCg        >  1:895916/1‑139 (MQ=255)
                                                                                                                            gAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGACGCGCTAAAGCGa       <  2:380270/139‑1 (MQ=255)
                                                                                                                            gAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGACGCGCTAAAGCGa       <  2:895916/139‑1 (MQ=255)
                                                                                                                            gAATCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGACGCGCTAAAGCGa       <  1:1286977/139‑1 (MQ=255)
                                                                                                                             aaTCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGt                              >  2:1409420/1‑115 (MQ=255)
                                                                                                                             aaTCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGt                              <  1:1409420/115‑1 (MQ=255)
                                                                                                                              aTCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGaca                                                                                      <  2:200664/58‑1 (MQ=255)
                                                                                                                              aTCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGaca                                                                                      >  1:200664/1‑58 (MQ=255)
                                                                                                                              aTCACAATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGACGCGCTAAAGCGAcg     >  1:1644072/1‑139 (MQ=255)
                                                                                                                                cacaATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGACGCGCTAAAGCGAcgcg   <  1:1771601/139‑1 (MQ=255)
                                                                                                                                 acaATCCCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGACGCGCTAAAGCGACgcgg  <  2:1644072/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                     
ATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAAT‑CCCCCCCTGCTCCGTATGCTGCGCCATACGCTGGGTCAGACGATCGACACCGTTAACGTCAATCGCGGTAAAAGGCTCGTCGAGGATCCATAACGTGGCACGGGTCAGCCACAGACGCGCTAAAGCGACGCGG  >  NC_000913/2296974‑2297240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A