Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I1 R1	759	71.0	3673240	91.5%	3361014	104.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,395,058	C→T	G331G (GGC→GGT)	nnr →	bifunctional NAD(P)H‑hydrate repair enzyme, C‑terminal domain ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase and N‑terminal domain NAD(P)H‑hydrate epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,395,058	0	C	T	100.0%	162.7 / NA	52	G331G (GGC→GGT)	nnr	bifunctional NAD(P)H‑hydrate repair enzyme, C‑terminal domain ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase and N‑terminal domain NAD(P)H‑hydrate epimerase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (30/22); total (30/22)

AGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCC  >  NC_000913/4394923‑4395186

aGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAg                                                                                                                               >  2:1692532/1‑139 (MQ=255)

 gTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGc                                                                                                                              <  1:818567/139‑1 (MQ=255)

 gTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGc                                                                                                                              <  1:727693/139‑1 (MQ=255)

      gACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAagag                                                                                                                         <  1:894484/139‑1 (MQ=255)

        cccGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTg                                                                                                                       >  1:1382856/1‑139 (MQ=255)

        cccGCAGTCAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAATTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTg                                                                                                                       <  1:1113003/139‑1 (MQ=255)

            cAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTggggg                                                                                                                   >  2:822893/1‑139 (MQ=255)

                       ttGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACt                                                                                                        >  1:988791/1‑139 (MQ=255)

                         gcgcCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGc                                                                                                      >  2:644797/1‑139 (MQ=255)

                                 gctgAATGCACGGCCGGAATTGATGGTGCATGAACTGACGATTGACTCTCTTACCGGAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGGCCCGGTCTGGGTCAGCAAGAGGTGGGGAAAAAAGCACTGCAAAAAGtt                                                                                              <  2:1382856/139‑1 (MQ=255)

                                          aCGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAAtttt                                                                                     >  1:141402/1‑139 (MQ=255)

                                          aCGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAAtttt                                                                                     >  1:938199/1‑139 (MQ=255)

                                            gACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCg                                                                                   <  1:1692532/139‑1 (MQ=255)

                                              ccGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCa                                                                                 >  1:1490332/1‑139 (MQ=255)

                                                   aTTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCg                                                                            <  1:1001408/139‑1 (MQ=255)

                                                        tGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAagag                                                                                                                         >  1:314696/1‑89 (MQ=255)

                                                        tGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAagag                                                                                                                         <  2:314696/89‑1 (MQ=255)

                                                            gCATGAACTGACGATGGACTCTCTCACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTGCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCg                                                                            <  1:183441/130‑1 (MQ=255)

                                                            gCATGAACTGACGATGGACTCTCTCACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCg                                                                            >  2:183441/1‑130 (MQ=255)

                                                                 aaCTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGc                                                              >  1:1703024/1‑139 (MQ=255)

                                                                  aCTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGcc                                                             >  2:911865/1‑139 (MQ=255)

                                                                     gACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGAt                                                          >  2:271851/1‑139 (MQ=255)

                                                                                     aCCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTgg                                          <  2:938199/139‑1 (MQ=255)

                                                                                         aaaGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTgggg                                                                                                                    <  1:55855/61‑1 (MQ=255)

                                                                                         aaaGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTgggg                                                                                                                    >  2:55855/1‑61 (MQ=255)

                                                                                           aGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaatca                                    >  2:1630170/1‑139 (MQ=255)

                                                                                           aGCCTGGAATGGGCCGATGTGGTGGTGACTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaatca                                    >  2:1135296/1‑139 (MQ=255)

                                                                                           aGCATGGAATGGGCCGATGGGGTGGTGATTGGTCCCGGTCTGGGTCAGCACGTGTGGGGGAAAAAAGAACTGCCAAAAGTAGAGAATTGTCGCAAATCGATGTTGTGGGTGGCCGATGCATTGAAACTGCTGGcaatct                                    >  1:388846/1‑138 (MQ=255)

                                                                                             ccTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaatcaat                                  <  1:644797/139‑1 (MQ=255)

                                                                                                    tGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATa                           <  2:141402/139‑1 (MQ=255)

                                                                                                      ggCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAg                         >  1:648243/1‑139 (MQ=255)

                                                                                                      ggCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAg                         >  2:1719999/1‑139 (MQ=255)

                                                                                                      ggCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAg                         >  2:370329/1‑139 (MQ=255)

                                                                                                       gCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAGAAGTTGAGAATTTTCGCAAACCGATGTTGTGTGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCg                              >  1:1191127/1‑133 (MQ=255)

                                                                                                       gCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAGAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAACCAATCCCg                              <  2:1191127/133‑1 (MQ=255)

                                                                                                       gCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGa                                                           >  2:1681429/1‑104 (MQ=255)

                                                                                                       gCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGa                                                           <  1:1681429/104‑1 (MQ=255)

                                                                                                       gCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGc                        >  2:493919/1‑139 (MQ=255)

                                                                                                        ccGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCg                       <  1:493919/139‑1 (MQ=255)

                                                                                                            tgtgGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTcac                   >  1:1518823/1‑139 (MQ=255)

                                                                                                                gtggtgATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCa                                                                                 >  2:1414688/1‑73 (MQ=255)

                                                                                                                gtggtgATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCa                                                                                 <  1:1414688/73‑1 (MQ=255)

                                                                                                                  ggtgATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATcgc             <  1:1630170/139‑1 (MQ=255)

                                                                                                                        tGGTCCCGGTCTGGGTCAGCAAGAGAGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATc       >  2:964955/1‑139 (MQ=255)

                                                                                                                          gTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaat                                      >  2:1026570/1‑106 (MQ=255)

                                                                                                                          gTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaat                                      <  1:1026570/106‑1 (MQ=255)

                                                                                                                          gTCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCAc     >  2:1531932/1‑139 (MQ=255)

                                                                                                                           tCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTtgtg                                                                    <  2:594749/75‑1 (MQ=255)

                                                                                                                           tCCCGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTtgtg                                                                    >  1:594749/1‑75 (MQ=255)

                                                                                                                             ccGGTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCAcgcc  <  1:1719999/139‑1 (MQ=255)

                                                                                                                                gTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGAt                            >  1:598272/1‑110 (MQ=255)

                                                                                                                                gTCTGGGTCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGAAGCATTGAACCTGCTGGCAATCAATCCCGAt                            <  2:598272/110‑1 (MQ=255)

AGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCC  >  NC_000913/4394923‑4395186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A