Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I2 R1
|
777 |
59.2 |
2955938 |
92.5% |
2734242 |
106.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,395,058 |
C→T |
G331G (GGC→GGT) |
nnr → |
bifunctional NAD(P)H‑hydrate repair enzyme, C‑terminal domain ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase and N‑terminal domain NAD(P)H‑hydrate epimerase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,395,058 | 0 | C | T | 100.0%
| 168.0
/ NA
| 52 | G331G (GGC→GGT) | nnr | bifunctional NAD(P)H‑hydrate repair enzyme, C‑terminal domain ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase and N‑terminal domain NAD(P)H‑hydrate epimerase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (29/23); total (29/23) |
AGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCC > NC_000913/4394923‑4395186
|
aGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAg > 2:380991/1‑139 (MQ=255)
gTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGc < 1:380991/139‑1 (MQ=255)
aCTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCaa < 1:896863/139‑1 (MQ=255)
aCCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGt > 1:405280/1‑139 (MQ=255)
aCCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCAGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGt < 1:187193/139‑1 (MQ=255)
ttGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACt < 2:405280/139‑1 (MQ=255)
aTTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCg < 2:165508/139‑1 (MQ=255)
gCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGTGGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTtgtg > 2:46654/1‑139 (MQ=255)
gCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑gg > 2:626971/1‑88 (MQ=255)
gCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑gg < 1:626971/88‑1 (MQ=255)
gCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTgg < 1:1189208/139‑1 (MQ=255)
gCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTgg > 1:952150/1‑139 (MQ=255)
aCTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGcc > 1:1469607/1‑139 (MQ=255)
cTGACGATGGACTCTCTTACCGAAAGCTTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAAATTTCGCAAACCGATGTTGTGGGATGCCg < 1:322079/139‑1 (MQ=255)
cTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGa > 2:291828/1‑134 (MQ=255)
cTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGa < 1:291828/134‑1 (MQ=255)
gACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑gg > 1:204798/1‑79 (MQ=255)
gACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑gg < 2:204798/79‑1 (MQ=255)
gACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGAt > 2:102602/1‑139 (MQ=255)
ggACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTg > 2:79355/1‑139 (MQ=255)
ctctTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTtgtg > 1:876147/1‑118 (MQ=255)
ctctTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTtgtg < 2:876147/118‑1 (MQ=255)
ctTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACctgc > 2:9910/1‑139 (MQ=255)
aaaGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaat < 1:102602/139‑1 (MQ=255)
aaaGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaat < 1:109001/139‑1 (MQ=255)
aaaGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaat > 2:1453138/1‑139 (MQ=255)
aaaGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaat > 2:109001/1‑139 (MQ=255)
aGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGA‑GTTGTGGGATGCCGATGCATTGAACCTGCTGGcaatcaa > 1:124954/1‑139 (MQ=255)
ccGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAAc > 2:275931/1‑84 (MQ=255)
ccGATGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAAc < 1:275931/84‑1 (MQ=255)
aTGTGGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTca > 1:1007371/1‑139 (MQ=255)
tgtgGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACctgctg < 2:966455/115‑1 (MQ=255)
tgtgGTGGTGATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACctgctg > 1:966455/1‑115 (MQ=255)
gtggtggtgATTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTcaca < 2:952150/139‑1 (MQ=255)
gtggtgATTGGTCCCGGTCTGGGTCAGCAAGAGT‑gg < 2:1030165/36‑1 (MQ=255)
gtggtgATTGGTCCCGGTCTGGGTCAGCAAGAGT‑gg > 1:1030165/1‑36 (MQ=255)
aTTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCg < 1:320758/65‑1 (MQ=255)
aTTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCg > 2:320758/1‑65 (MQ=255)
aTTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGa > 2:1387225/1‑139 (MQ=255)
aTTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCCGCTGGCAATCAATCCCGATa > 1:124456/1‑121 (MQ=255)
aTTGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCCGCTGGCAATCAATCCCGATa < 2:124456/121‑1 (MQ=255)
tGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTCGTGGGATGCCGATGCa < 1:752000/91‑1 (MQ=255)
tGGTCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTCGTGGGATGCCGATGCa > 2:752000/1‑91 (MQ=255)
tCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAACAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCAcg > 1:1042918/1‑139 (MQ=255)
tCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAAcc > 2:515481/1‑66 (MQ=255)
tCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAAcc < 1:515481/66‑1 (MQ=255)
tCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCAcg > 1:1028972/1‑139 (MQ=255)
tCCCGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCAcg > 1:728621/1‑139 (MQ=255)
ccGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCAcgcc > 2:884440/1‑139 (MQ=255)
ccGGTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCAcgcc < 1:79355/139‑1 (MQ=255)
gTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCaaa < 2:483733/59‑1 (MQ=255)
gTCTGGGTCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCaaa > 1:483733/1‑59 (MQ=255)
|
AGTACTGACCCGCAGTGAAAACATTGCGCCGCTGCTGACTGCACGACCGGAATTGATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCCGATAAGCGTCACAATCGCGTGATCACGCC > NC_000913/4394923‑4395186
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
