Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I1 R1	760	58.2	3022526	90.8%	2744453	102.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	679,874	G→A	G123R (GGG→AGG)	ybeU →	DUF1266 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	679,874	0	G	A	100.0%	129.4 / NA	41	G123R (GGG→AGG)	ybeU	DUF1266 family protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (18/23); total (18/23)

CCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTA  >  NC_000913/679747‑679989

cccTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCa                                                                                                          <  2:290075/139‑1 (MQ=255)

     aaGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGt                                                                                                     >  1:802179/1‑139 (MQ=255)

       gagTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCg                                                                                                   >  1:26664/1‑139 (MQ=255)

          tGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGgtg                                                                                                >  2:195949/1‑139 (MQ=255)

          tGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGgtg                                                                                                >  1:1306905/1‑139 (MQ=255)

              aaGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCt                                                                                            <  2:802179/139‑1 (MQ=255)

                            ggtggCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGa                                                                              <  1:1042918/139‑1 (MQ=255)

                                gCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGga                                                                          <  2:1306905/139‑1 (MQ=255)

                                    ggAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCACCAAGTGGCTGACGgaagaa                                                                      <  1:1004789/139‑1 (MQ=255)

                                                              tCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGt                                            <  2:26664/139‑1 (MQ=255)

                                                                   gCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCAc                                                                                                         >  2:602816/1‑73 (MQ=255)

                                                                   gCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCAc                                                                                                         <  1:602816/73‑1 (MQ=255)

                                                                   gCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATg                                       >  1:749189/1‑139 (MQ=255)

                                                                        aaCCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGt                                            >  2:1123510/1‑129 (MQ=255)

                                                                        aaCCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGt                                            <  1:1123510/129‑1 (MQ=255)

                                                                              tATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGTAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCAc                            <  2:682079/139‑1 (MQ=255)

                                                                              tATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCAc                            >  1:868797/1‑139 (MQ=255)

                                                                                 gtgCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCAt                         <  2:77560/139‑1 (MQ=255)

                                                                                 gtgCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCAt                         <  2:1150018/139‑1 (MQ=255)

                                                                                   ggtggtgggAAGGGGGAATTAAAGCCTGGGATTATGTTTGAATGAGGTTTCTGTCACGGGGCCGTGTGCTCAACAAGTGGGTGGCGGAAAAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTa                       <  2:79470/133‑1 (MQ=255)

                                                                                     tgtgGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCg                                                                                                   >  2:1304717/1‑61 (MQ=255)

                                                                                     tgtgGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCg                                                                                                   <  1:1304717/61‑1 (MQ=255)

                                                                                        ggggAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATcgcg                                               <  1:1184891/110‑1 (MQ=255)

                                                                                        ggggAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATcgcg                                               >  2:1184891/1‑110 (MQ=255)

                                                                                            aaGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAagaaga                                                                    <  2:1357004/85‑1 (MQ=255)

                                                                                            aaGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAagaaga                                                                    >  1:1357004/1‑85 (MQ=255)

                                                                                            aaGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCt                               <  2:454281/122‑1 (MQ=255)

                                                                                            aaGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCt                               >  1:454281/1‑122 (MQ=255)

                                                                                            aaGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTAt                   <  2:1148661/134‑1 (MQ=255)

                                                                                            aaGGGGGAATCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTAt                   >  1:1148661/1‑134 (MQ=255)

                                                                                                   aaTCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATcgcgc                                              >  2:1301055/1‑100 (MQ=255)

                                                                                                   aaTCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATcgcgc                                              <  1:1301055/100‑1 (MQ=255)

                                                                                                    aTCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCa                                                                                                          <  2:319483/39‑1 (MQ=255)

                                                                                                    aTCAAAGCCTGGGATTATGTTCGAATGAGGTTTCTGTCa                                                                                                          >  1:319483/1‑39 (MQ=255)

                                                                                                        aaGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTa  <  2:868797/139‑1 (MQ=255)

                                                                                                         aGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACgg                                                                           <  2:1419092/65‑1 (MQ=255)

                                                                                                         aGCCTGGGATTATGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACgg                                                                           >  1:1419092/1‑65 (MQ=255)

                                                                                                                     tGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGc                                <  1:625230/96‑1 (MQ=255)

                                                                                                                     tGTTCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGc                                >  2:625230/1‑96 (MQ=255)

                                                                                                                       ttCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGt                                            <  1:717966/82‑1 (MQ=255)

                                                                                                                       ttCGAATGAGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGt                                            >  2:717966/1‑82 (MQ=255)

CCCTGAAGAGTGGAAAGCATTAATTGCTGGTGGCTCGGAGAGAGGGTTGATTTATACCCAATTCGTTGCCCAAACCGCTATGTGCTGTGGGGAAGGGGGAATCAAAGCCTGGGATTATGTTCGAATGGGGTTTCTGTCACGGGTCGGTGTGCTCAACAAGTGGCTGACGGAAGAAGAGAGTTTATGGCTGCAATCGCGCGTCTATGTACGAGCTCACCATTACTATCACAGCTGGATGCACTA  >  NC_000913/679747‑679989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A