Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I1 R1	760	58.2	3022526	90.8%	2744453	102.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,549,687	A→G	N763D (AAT→GAT)	fdnG →	formate dehydrogenase‑N, alpha subunit, nitrate‑inducible

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,549,687	0	A	G	100.0%	177.8 / NA	49	N763D (AAT→GAT)	fdnG	formate dehydrogenase‑N, alpha subunit, nitrate‑inducible
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (26/23); total (26/23)

CATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGA  >  NC_000913/1549573‑1549816

cATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGt                                                                                                           >  1:579753/1‑139 (MQ=255)

   cTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGc                                                                                                        >  2:43065/1‑139 (MQ=255)

      cGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGc                                                                                                     >  2:71332/1‑139 (MQ=255)

              cGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGt                                                                                             >  1:1031531/1‑139 (MQ=255)

               ggTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTc                                                                                            <  2:579753/139‑1 (MQ=255)

                gTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGc                                                                                                                   >  1:432395/1‑115 (MQ=255)

                gTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACGCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGc                                                                                                                   <  2:432395/115‑1 (MQ=255)

                  aCAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTcgcg                                                                                         <  2:1031531/139‑1 (MQ=255)

                    aaCCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTg                                                                                       >  1:723233/1‑139 (MQ=255)

                         cATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGc                                                                                                                   >  1:454861/1‑106 (MQ=255)

                         cATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGc                                                                                                                   <  2:454861/106‑1 (MQ=255)

                               cttGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCg                                                                            <  1:71332/139‑1 (MQ=255)

                               cttGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCg                                                                            >  1:1447499/1‑139 (MQ=255)

                                 tGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTg                                                                          >  2:211522/1‑139 (MQ=255)

                                       aTCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTcgg                                                                    >  1:1322548/1‑139 (MQ=255)

                                          tACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTcggcgg                                                                 <  1:43065/139‑1 (MQ=255)

                                            cacCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGat                                                               <  2:1322548/139‑1 (MQ=255)

                                                      tGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGatat                                                             >  1:70094/1‑131 (MQ=255)

                                                      tGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGatat                                                             <  2:70094/131‑1 (MQ=255)

                                                      tGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTa                                                     >  1:408344/1‑139 (MQ=255)

                                                            gagCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGt                                               <  1:211522/139‑1 (MQ=255)

                                                             agCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCtt                                                                       <  1:27628/114‑1 (MQ=255)

                                                             agCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCtt                                                                       >  2:27628/1‑114 (MQ=255)

                                                             agCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTg                                              >  1:178836/1‑139 (MQ=255)

                                                                  ggCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTc                                                                      >  1:707476/1‑110 (MQ=255)

                                                                  ggCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTc                                                                      <  2:707476/110‑1 (MQ=255)

                                                                  ggCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGt                                                      >  1:1406839/1‑126 (MQ=255)

                                                                  ggCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGt                                                      <  2:1406839/126‑1 (MQ=255)

                                                                  ggCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATc                                         >  2:84745/1‑139 (MQ=255)

                                                                       ccAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCtt                                                                       >  2:934974/1‑104 (MQ=255)

                                                                       ccAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCtt                                                                       <  1:934974/104‑1 (MQ=255)

                                                                              gCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGc                             >  2:547964/1‑139 (MQ=255)

                                                                                 aaCCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATcc                                        >  2:629706/1‑125 (MQ=255)

                                                                                 aaCCGCGATAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATcc                                        <  1:629706/125‑1 (MQ=255)

                                                                                         tAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTgg                                             >  1:545360/1‑112 (MQ=255)

                                                                                         tAACTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTgg                                             <  2:545360/112‑1 (MQ=255)

                                                                                           aCTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACCGATGCTGATCCAGTGGaa                <  2:836376/139‑1 (MQ=255)

                                                                                            cTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGt                                               <  2:1378535/107‑1 (MQ=255)

                                                                                            cTCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGt                                               >  1:1378535/1‑107 (MQ=255)

                                                                                             tCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGc                                                                                                                   >  2:1165598/1‑38 (MQ=255)

                                                                                             tCCGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGc                                                                                                                   <  1:1165598/38‑1 (MQ=255)

                                                                                              ccGACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACgg             <  2:408344/139‑1 (MQ=255)

                                                                                                gACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACg                                  >  2:1216842/1‑116 (MQ=255)

                                                                                                gACCCGTCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACg                                  <  1:1216842/116‑1 (MQ=255)

                                                                                                      tCCGGTCTGGGGGATACTCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGatat                                                             <  2:1030821/83‑1 (MQ=255)

                                                                                                      tCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGt                                                                                                           >  1:1295361/1‑37 (MQ=255)

                                                                                                      tCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGt                                                                                                           <  2:1295361/37‑1 (MQ=255)

                                                                                                      tCCGGTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGatat                                                             >  1:1030821/1‑83 (MQ=255)

                                                                                                         ggTCTGGGGGATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGa  <  1:547964/139‑1 (MQ=255)

CATCTGCGTGATGACGGTACAACCGCATCTTCTTGCTGGATCTACACCGGTAGCTGGACAGAGCAGGGCAACCAGATGGCTAACCGCGATAACTCCGACCCGTCCGGTCTGGGGAATACGCTGGGATGGGCCTGGGCGTGGCCGCTCAACCGTCGCGTGCTGTACAACCGTGCTTCGGCGGATATCAACGGTAAACCGTGGGATCCGAAACGGATGCTGATCCAGTGGAACGGCAGCAAGTGGA  >  NC_000913/1549573‑1549816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A