Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I2 R1
|
758 |
59.3 |
2864096 |
93.9% |
2689386 |
105.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
414,875 |
A→G |
R293R (CGT→CGC) |
sbcC ← |
exonuclease, dsDNA, ATP‑dependent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 414,875 | 0 | A | G | 100.0%
| 149.3
/ NA
| 42 | R293R (CGT→CGC) | sbcC | exonuclease, dsDNA, ATP‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (20/22); total (20/22) |
GACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGACGGCGGCTGGCTTCTTGCTGCAATTCGTCCTGACGCGTTAAC > NC_000913/414741‑415010
|
gACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGaa > 2:1078829/1‑139 (MQ=255)
cgccgcGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGt < 1:1084983/139‑1 (MQ=255)
gccgcGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTg < 1:932187/139‑1 (MQ=255)
cgTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCgg < 1:739348/139‑1 (MQ=255)
cTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTg > 2:218104/1‑121 (MQ=255)
cTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTg < 1:218104/121‑1 (MQ=255)
cgcgcgAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAgc < 1:1117535/139‑1 (MQ=255)
ctctGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTTCCgg > 1:715669/1‑104 (MQ=255)
ctctGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCgg < 2:715669/104‑1 (MQ=255)
tATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTACCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTatcttcttc > 1:849699/1‑139 (MQ=255)
cttcAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGc < 1:1078829/139‑1 (MQ=255)
ttcAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGcgccg < 1:1093018/101‑1 (MQ=255)
ttcAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGcgccg > 2:1093018/1‑101 (MQ=255)
ttcAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGcc < 1:821785/139‑1 (MQ=255)
ttcAATCTGCTGGCGAATATGCACCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGtt < 2:548174/83‑1 (MQ=255)
ttcAATCTGCTGGCGAATATGCACCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGtt > 1:548174/1‑83 (MQ=255)
tcAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCgg > 2:1287195/1‑80 (MQ=255)
tcAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCgg < 1:1287195/80‑1 (MQ=255)
tcAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTgc < 1:482750/114‑1 (MQ=255)
tcAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTgc > 2:482750/1‑114 (MQ=255)
ctgctgGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTCGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGc < 2:849699/139‑1 (MQ=255)
ctgGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCgg > 2:1173532/1‑72 (MQ=255)
ctgGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCgg < 1:1173532/72‑1 (MQ=255)
ctgGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTgc < 2:85530/106‑1 (MQ=255)
ctgGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTgc > 1:85530/1‑106 (MQ=255)
ctgGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAg > 2:290714/1‑139 (MQ=255)
tgGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAgg > 2:561805/1‑139 (MQ=255)
gcCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTaa > 2:1005129/1‑113 (MQ=255)
gcCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTaa < 1:1005129/113‑1 (MQ=255)
gcCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCaa > 1:1308669/1‑125 (MQ=255)
gcCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCaa < 2:1308669/125‑1 (MQ=255)
gcCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGAcgg < 1:725669/138‑1 (MQ=255)
gcCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGAcgg > 2:725669/1‑138 (MQ=255)
cAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGAcggcgg > 2:913790/1‑139 (MQ=255)
ccgcGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCttttt < 2:976790/93‑1 (MQ=255)
ccgcGCTGTGTTCTGCGATGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCttttt > 1:976790/1‑93 (MQ=255)
tGCGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGACGGCGGCTGGCTTCTTGCTGCAATTCGTc > 2:35019/1‑139 (MQ=255)
cGTTCCCAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGACGGCGGCTGGCTTCTTGCTGCAATTCGTCCt > 1:1009308/1‑139 (MQ=255)
ccAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGACGGCGGCTGGcttc < 2:1123577/117‑1 (MQ=255)
ccAGTGTGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGACGGCGGCTGGcttc > 1:1123577/1‑117 (MQ=255)
gtgtGGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGACGGCGGCTGGCTTCTTGCTGCAATTCGTCCTGACGCGtt < 1:561805/139‑1 (MQ=255)
tgGGCGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGACGGCGGCTGGCTTCTTGCTGCAATTCGTCCTGACGCGTTAAc < 1:290714/139‑1 (MQ=255)
|
GACTGCTTCGCCGCGTGGTGGCGAATGCTCGCGCGAAGCGCCATTGTGCTCTGTAAGCGAGTATTTACTTCTTCAATCTGCTGGCGAATATGCGCCAGCGCCGCGCTGTGTTCTGCGATGCGTTCCCAGTGTGGACGAAGATTTCGTGCCGGTTGTGCCAGACTAAGCGCCGCCAGTTGAGGTTGCGCTTTTTCTTCTTCGGCTAACGCCTGTTGCAAGGCCTGCTGACGGCGGCTGGCTTCTTGCTGCAATTCGTCCTGACGCGTTAAC > NC_000913/414741‑415010
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
