Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I2 R1 758 59.3 2864096 93.9% 2689386 105.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 486,641 T→C S36P (TCA→CCA)  mscK → mechanosensitive channel protein, intermediate conductance, K+ regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913486,6410TC100.0% 237.5 / NA 66S36P (TCA→CCA) mscKmechanosensitive channel protein, intermediate conductance, K+ regulated
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/34);  total (32/34)

GTTCAGAAACCTTCATTCATCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAGATACATTAGCCACCCT  >  NC_000913/486515‑486768
                                                                                                                              |                                                                                                                               
gTTCAGAAACCTTCATTCATCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTg                                                                                                                     >  2:409565/1‑139 (MQ=255)
   cAGAAACCTTCATTCATCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATc                                                                                                                  >  2:1169892/1‑139 (MQ=255)
       aaCCTTCATTCATCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGcc                                                                                                              <  1:1152197/139‑1 (MQ=255)
                            tGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCa                                                                                                                               <  2:1394605/101‑1 (MQ=255)
                            tGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCa                                                                                            <  1:1199352/136‑1 (MQ=255)
                            tGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCa                                                                                            >  2:1199352/1‑136 (MQ=255)
                            tGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGgc                                                                                         >  1:52680/1‑139 (MQ=255)
                            tGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGgc                                                                                         >  1:395436/1‑139 (MQ=255)
                            tGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGgc                                                                                         >  2:398440/1‑139 (MQ=255)
                            tGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGgc                                                                                         >  1:1401028/1‑139 (MQ=255)
                            tGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGGTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGgc                                                                                         >  1:528177/1‑139 (MQ=255)
                               tCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTg                                                                                                                >  1:672512/1‑113 (MQ=255)
                               tCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTg                                                                                                                <  2:672512/113‑1 (MQ=255)
                                 cAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCa                                                                                                                               <  2:451598/96‑1 (MQ=255)
                                 cAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAgg                                                                                          <  2:1178381/133‑1 (MQ=255)
                                 cAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAgg                                                                                          >  1:1178381/1‑133 (MQ=255)
                                    tATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTg                                                                                 <  1:1299524/139‑1 (MQ=255)
                                    tATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTg                                                                                 <  1:409565/139‑1 (MQ=255)
                                    tATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTg                                                                                 >  1:1070564/1‑139 (MQ=255)
                                        aCAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTc                                                                             >  2:338326/1‑139 (MQ=255)
                                        aCAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTc                                                                             >  2:228216/1‑139 (MQ=255)
                                        aCAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTc                                                                             <  2:395436/139‑1 (MQ=255)
                                        aCAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTc                                                                             <  1:338326/139‑1 (MQ=255)
                                              gATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGAc                                                                                                           >  1:853342/1‑103 (MQ=255)
                                              gATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGAc                                                                                                           <  2:853342/103‑1 (MQ=255)
                                              gATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACaaaa                                                                       >  2:467411/1‑139 (MQ=255)
                                                   cGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATc                                                                                                                             <  1:1305684/80‑1 (MQ=255)
                                                   cGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATc                                                                                                                             >  2:1305684/1‑80 (MQ=255)
                                                        ttttGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTg                                                                                              <  1:1027972/106‑1 (MQ=255)
                                                        ttttGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTg                                                                                              >  2:1027972/1‑106 (MQ=255)
                                                          ttGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGa                                                           <  1:228216/139‑1 (MQ=255)
                                                              tttttCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACT‑AATAAACAAAAAGATCttt                                                      <  2:52680/139‑1 (MQ=255)
                                                                    aGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCt                                                 <  2:682711/139‑1 (MQ=255)
                                                                     gCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGa                                                                                >  1:792354/1‑107 (MQ=255)
                                                                     gCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGa                                                                                <  2:792354/107‑1 (MQ=255)
                                                                       aTTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTg                                                                                              <  1:1109915/91‑1 (MQ=255)
                                                                       aTTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTg                                                                                              >  2:1109915/1‑91 (MQ=255)
                                                                           aTTACTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGAc                                                                               <  2:712487/102‑1 (MQ=255)
                                                                           aTTACTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGAc                                                                               >  1:712487/1‑102 (MQ=255)
                                                                                ttttgtttttgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCt                                                 <  2:1409019/127‑1 (MQ=255)
                                                                                ttttgtttttgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCt                                                 >  1:1409019/1‑127 (MQ=255)
                                                                                ttttgtttttgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGa                                            >  1:271511/1‑132 (MQ=255)
                                                                                ttttgtttttgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGa                                            <  2:271511/132‑1 (MQ=255)
                                                                                 tttgtttttgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGAt                                                                                                                   <  2:723447/60‑1 (MQ=255)
                                                                                 tttgtttttgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGAt                                                                                                                   >  1:723447/1‑60 (MQ=255)
                                                                                   tgtttttgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCt                                                 <  2:1237239/124‑1 (MQ=255)
                                                                                   tgtttttgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCt                                                 >  1:1237239/1‑124 (MQ=255)
                                                                                      ttttgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTgcag                               <  2:1401028/139‑1 (MQ=255)
                                                                                      ttttgtCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTgcag                               <  2:1070564/139‑1 (MQ=255)
                                                                                                 tATGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGAc                    <  1:398440/139‑1 (MQ=255)
                                                                                                   tGCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAg                  >  2:1241007/1‑139 (MQ=255)
                                                                                                    gCCAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAGa                 <  1:1169892/139‑1 (MQ=255)
                                                                                                     ccAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCt                                                 >  1:194152/1‑106 (MQ=255)
                                                                                                     ccAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCt                                                 <  2:194152/106‑1 (MQ=255)
                                                                                                     ccAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTgcagca                             <  1:633797/126‑1 (MQ=255)
                                                                                                     ccAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTgcagca                             >  2:633797/1‑126 (MQ=255)
                                                                                                      cAGAACACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAGATa               <  2:528177/139‑1 (MQ=255)
                                                                                                         aaCACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAGATACa             <  2:1304736/138‑1 (MQ=255)
                                                                                                         aaCACGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAGATACa             >  1:1304736/1‑138 (MQ=255)
                                                                                                          acacGGCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAGATACAtt           >  1:928080/1‑139 (MQ=255)
                                                                                                              ggCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGgcg                                                                                        >  1:80672/1‑58 (MQ=255)
                                                                                                              ggCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGgcg                                                                                        <  2:80672/58‑1 (MQ=255)
                                                                                                               gCGTTTGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAGATACATTAGCCa      >  1:626265/1‑139 (MQ=255)
                                                                                                                   ttGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTATATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAGATACATTAGCCACCCt  <  1:467411/139‑1 (MQ=255)
                                                                                                                   ttGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACaaaaa                                                             >  2:1370318/1‑80 (MQ=255)
                                                                                                                   ttGCGCGGGCGCCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACaaaaa                                                             <  1:1370318/80‑1 (MQ=255)
                                                                                                                              |                                                                                                                               
GTTCAGAAACCTTCATTCATCATGACTATGTTCCAGTATTACAAACGATCACGGCATTTTGTTTTTTCAGCATTTATTGCTTTTGTTTTTGTCTTGTTATGCCAGAACACGGCGTTTGCGCGGGCGTCATCGAATGGTGATCTGCCGACAAAAGCGGACCTGCAGGCGCAACTTGACTCACTAAATAAACAAAAAGATCTTTCTGCTCAGGACAAACTGGTGCAGCAGGATCTGACAGATACATTAGCCACCCT  >  NC_000913/486515‑486768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A