Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I2 R1 758 59.3 2864096 93.9% 2689386 105.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,189,927 A→G L174S (TTA→TCA)  phoP ← response regulator in two‑component regulatory system with PhoQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,189,9270AG98.7% 278.5 / NA 77L174S (TTA→TCA) phoPresponse regulator in two‑component regulatory system with PhoQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (37/39);  minor base C (0/1);  total (37/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGG  >  NC_000913/1189803‑1190058
                                                                                                                            |                                                                                                                                   
gCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTg                                                                                                                       <  1:653369/139‑1 (MQ=255)
gCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTg                                                                                                                       >  2:425567/1‑139 (MQ=255)
gCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTg                                                                                                                       >  2:863400/1‑139 (MQ=255)
 ccGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGa                                                                                                                      <  1:847976/139‑1 (MQ=255)
     gAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCAc                                                                                                                  >  2:1364773/1‑139 (MQ=255)
                aTCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCattat                                                                                                       >  2:1270210/1‑139 (MQ=255)
                 tCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCattatt                                                                                                      <  1:1364773/139‑1 (MQ=255)
                       cttGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTAt                                                                                                >  1:343678/1‑139 (MQ=255)
                           gggATATTGTGCCTGAATTTTTTTGCGCAGACGTCTCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTACCATTATTGCGTATCAAc                                                                                            <  1:863400/139‑1 (MQ=255)
                           gggATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAAc                                                                                            <  2:343678/139‑1 (MQ=255)
                           gggATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAAc                                                                                            <  2:6384/139‑1 (MQ=255)
                           gggATATTGTGCCTGAATTTTCGTCCGCAGTCGCCCCATCAGAACATCAATGGTATGGCTATCCCGCAGCTCCGCATCCGGATAGAGTGGTAACAGTCACGAAGCTTTTCTGAGCACTTGTCCATTATTGCGTATCAAc                                                                                            <  2:795726/139‑1 (MQ=255)
                            ggATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACg                                                                                                                                  <  1:990880/100‑1 (MQ=255)
                            ggATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACg                                                                                                                                  >  2:990880/1‑100 (MQ=255)
                               tatTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGttt                                                                                        >  2:890235/1‑139 (MQ=255)
                               tatTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGttt                                                                                        <  2:653063/139‑1 (MQ=255)
                                   gtgCCTGAATTGTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCat                                                                                    <  2:983797/139‑1 (MQ=255)
                                      ccTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGt                                                                                                  <  1:951256/122‑1 (MQ=255)
                                      ccTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGt                                                                                                  >  2:951256/1‑122 (MQ=255)
                                      ccTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGCTTCCataat                                                                                 >  1:476160/1‑139 (MQ=255)
                                       cTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCatta                                                                                                        >  1:770352/1‑115 (MQ=255)
                                       cTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCatta                                                                                                        <  2:770352/115‑1 (MQ=255)
                                       cTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAAc                                                                                            >  1:654148/1‑127 (MQ=255)
                                       cTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAAc                                                                                            <  2:654148/127‑1 (MQ=255)
                                       cTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGttt                                                                                        <  2:441334/131‑1 (MQ=255)
                                       cTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGttt                                                                                        >  1:441334/1‑131 (MQ=255)
                                       cTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCataata                                                                                <  2:477731/139‑1 (MQ=255)
                                          aaTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAgtg                                                                             >  2:600552/1‑139 (MQ=255)
                                          aaTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAg                                                                               <  1:1212961/137‑1 (MQ=255)
                                          aaTTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAg                                                                               >  2:1212961/1‑137 (MQ=255)
                                                         aCGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGAc                                                                                                                     <  1:853933/84‑1 (MQ=255)
                                                         aCGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAAc                                                                                            <  2:46651/109‑1 (MQ=255)
                                                         aCGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAAc                                                                                            >  1:46651/1‑109 (MQ=255)
                                                         aCGTCCCATCAGTACATCAATGGTATGACTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGAc                                                                                                                     >  2:853933/1‑84 (MQ=255)
                                                              ccATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGt                                                                                          >  1:490752/1‑106 (MQ=255)
                                                              ccATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGt                                                                                          <  2:490752/106‑1 (MQ=255)
                                                                   aGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCattatt                                                                                                      <  1:746372/89‑1 (MQ=255)
                                                                   aGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCattatt                                                                                                      >  2:746372/1‑89 (MQ=255)
                                                                   aGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAAc                                                                                            >  2:1311929/1‑99 (MQ=255)
                                                                   aGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAAc                                                                                            <  1:1311929/99‑1 (MQ=255)
                                                                     tACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACt                                                  >  2:523273/1‑139 (MQ=255)
                                                                         tCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGt                                              <  1:890235/139‑1 (MQ=255)
                                                                          cAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTTCGTATCAACGTTTCCATAATAGTGTATTCGAACGCgg                                                                >  1:350842/1‑120 (MQ=255)
                                                                          cAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCgg                                                                <  2:350842/120‑1 (MQ=255)
                                                                          cAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGt                                              >  1:784816/1‑138 (MQ=255)
                                                                          cAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGt                                              <  2:784816/138‑1 (MQ=255)
                                                                           aaTGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCa                                            <  1:1270210/139‑1 (MQ=255)
                                                                            aTGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCa                                                                                                           <  2:1047129/75‑1 (MQ=255)
                                                                            aTGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCa                                                                                                           >  1:1047129/1‑75 (MQ=255)
                                                                             tGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGtt                                                                                         >  1:358172/1‑92 (MQ=255)
                                                                             tGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGtt                                                                                         <  2:358172/92‑1 (MQ=255)
                                                                             tGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCAtt                                          >  2:104638/1‑139 (MQ=255)
                                                                              ggTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTa                                         <  1:104638/139‑1 (MQ=255)
                                                                              ggTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGCTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTa                                         <  2:476160/139‑1 (MQ=255)
                                                                               gTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGt                                                                                          <  1:649565/89‑1 (MQ=255)
                                                                               gTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGt                                                                                          >  2:649565/1‑89 (MQ=255)
                                                                               gTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCgg                                                                >  2:1170952/1‑115 (MQ=255)
                                                                               gTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCgg                                                                <  1:1170952/115‑1 (MQ=255)
                                                                               gTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTaa                                        >  1:141042/1‑139 (MQ=255)
                                                                                     cTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTc                                                >  1:856856/1‑125 (MQ=255)
                                                                                     cTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTc                                                <  2:856856/125‑1 (MQ=255)
                                                                                          ccGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGc                                                                                                             <  1:1005194/59‑1 (MQ=255)
                                                                                          ccGCAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGc                                                                                                             >  2:1005194/1‑59 (MQ=255)
                                                                                             cAGCTCCGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACgg                          <  1:425567/139‑1 (MQ=255)
                                                                                                   cGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCa                             >  2:1096265/1‑130 (MQ=255)
                                                                                                   cGCATCCGGATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCa                             <  1:1096265/130‑1 (MQ=255)
                                                                                                      aTCCGGATAGAGTTGTATCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGAATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCgagagaga                 <  1:515305/139‑1 (MQ=255)
                                                                                                           gATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAAc                                                                                            <  2:608105/59‑1 (MQ=255)
                                                                                                           gATAGAGTTGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAAc                                                                                            >  1:608105/1‑59 (MQ=255)
                                                                                                                  ttGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGttt                                                                                        >  2:1382725/1‑56 (MQ=255)
                                                                                                                  ttGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGttt                                                                                        <  1:1382725/56‑1 (MQ=255)
                                                                                                                  ttGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGaa                                                                     <  2:475544/75‑1 (MQ=255)
                                                                                                                  ttGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGaa                                                                     >  1:475544/1‑75 (MQ=255)
                                                                                                                  ttGGAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAAc     >  2:624840/1‑139 (MQ=255)
                                                                                                                     gAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAAc                                                                                            <  2:767142/49‑1 (MQ=255)
                                                                                                                     gAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAAc                                                                                            >  1:767142/1‑49 (MQ=255)
                                                                                                                     gAGCATTGACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACggg  >  1:1244725/1‑139 (MQ=255)
                                                                                                                            |                                                                                                                                   
GCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGG  >  NC_000913/1189803‑1190058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A