Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F1 I2 R1
|
762 |
35.5 |
1545288 |
95.5% |
1475750 |
117.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,190,043 |
(GA)4→5 |
coding (405/672 nt) |
phoP ← |
response regulator in two‑component regulatory system with PhoQ |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,190,035 | 1 | . | G | 100.0%
| 108.5
/ NA
| 32 | R138P (CGC→CCC) | phoP | response regulator in two‑component regulatory system with PhoQ |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (18/14); total (18/14) |
| * | NC_000913 | 1,190,035 | 2 | . | A | 100.0%
| 100.8
/ NA
| 32 | R138L (CGC→CTC) | phoP | response regulator in two‑component regulatory system with PhoQ |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (18/14); total (18/14) |
AGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGC‑‑GAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAG > NC_000913/1189897‑1190164
||
aGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGc < 1:724940/139‑1 (MQ=255)
cTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGC‑‑ga > 2:622937/1‑139 (MQ=255)
ggATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGC‑‑gagagagagatc < 1:662252/139‑5 (MQ=255)
gAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACg > 2:279370/1‑139 (MQ=255)
ttAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACgggggg > 2:161078/1‑139 (MQ=255)
aaTCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGaa < 2:625065/139‑1 (MQ=255)
tttGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGa < 2:189292/139‑1 (MQ=255)
gCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCt > 1:168400/1‑139 (MQ=255)
cACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAg < 2:263738/139‑1 (MQ=255)
aCTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGc < 1:417626/139‑1 (MQ=255)
tttGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCa > 1:519644/1‑139 (MQ=255)
cGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTAcg < 1:325228/139‑1 (MQ=255)
taGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAg > 1:65805/1‑108 (MQ=255)
taGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAg < 2:65805/108‑1 (MQ=255)
taGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCAtt > 2:439753/1‑139 (MQ=255)
tgtATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGc > 1:466088/1‑102 (MQ=255)
tgtATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGc < 2:466088/102‑1 (MQ=255)
tgtATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTcgc > 1:229137/1‑139 (MQ=255)
tATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCgcgc > 2:768192/1‑139 (MQ=255)
tATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCgcgc > 2:401044/1‑139 (MQ=255)
tATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCgcgc > 1:214444/1‑139 (MQ=255)
tATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCgcgc > 1:250101/1‑139 (MQ=255)
aTTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGcc < 1:599557/139‑1 (MQ=255)
tCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCAt > 1:303637/1‑139 (MQ=255)
tCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGAGCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCAt < 2:10198/139‑1 (MQ=255)
aaCGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCAc < 1:401044/139‑1 (MQ=255)
aaCGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCAc < 2:229137/139‑1 (MQ=255)
aaCGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCAc < 1:161078/139‑1 (MQ=255)
aCGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGC‑‑gagag < 2:266899/50‑1 (MQ=255)
aCGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGC‑‑gagag > 1:266899/1‑50 (MQ=255)
cgcgGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTAcgcc < 2:259099/109‑1 (MQ=255)
cgcgGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTAcgcc > 1:259099/1‑109 (MQ=255)
cgcgGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACct > 1:11235/1‑139 (MQ=255)
tCGTCATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGttt > 1:648740/1‑139 (MQ=255)
cATTAATAGATAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTc < 1:759562/139‑1 (MQ=255)
aTAATTCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAatca > 1:233544/1‑139 (MQ=255)
ttCACGGCGAGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGAACTGTGAAGCCAGACCGCTATTACGCCGCATTAATGTCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCAAATATTCATTAg > 2:573994/1‑139 (MQ=255)
||
AGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGTATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGC‑‑GAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAG > NC_000913/1189897‑1190164
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
