Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I2 R1	758	59.3	2864096	93.9%	2689386	105.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,366,935	C→T	326 (TAG→TAA)	pspF ←	psp operon transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,366,935	0	C	T	100.0%	244.4 / NA	75	326 (TAG→TAA)	pspF	psp operon transcriptional activator
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (38/37); total (38/37)

ATGCGCTGAGCGATTAATAGCCCAACGCAATAATGTCTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGCTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAA  >  NC_000913/1366799‑1367069

aTGCGCTGAGCGATTAATAGCCCAACGCAATAATGTCTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTa                                                                                                                                      >  1:1426757/1‑139 (MQ=255)

        aGCGATTAATAGCCCAACGCAATAATGTCTGATGCGCTGCGCTTATCAGGCCTGCAAACGATGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGt                                                                                                                              <  1:881971/139‑1 (MQ=255)

             ttAATAGCCCAACGCAATAATGTCTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCttt                                                                                                                         >  2:886723/1‑139 (MQ=255)

               aaTAGCCCAACGCAATAATGTCTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCttttt                                                                                                                       >  1:43452/1‑139 (MQ=255)

                    cccAACGCAATAATGTCTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTcaaca                                                                                                                  <  1:1107939/139‑1 (MQ=255)

                      cAACGCAATAATGTCTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCaacaac                                                                                                                >  2:18866/1‑139 (MQ=255)

                       aaCGCAATAATGTCTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATGTGGTGCTTTTTCAACAAcg                                                                                                               >  1:1271378/1‑139 (MQ=255)

                         cGCAATAATGTCTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAAcgcg                                                                                                             <  1:18866/139‑1 (MQ=255)

                         cGCAATAATGTCTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAAcg                                                                                                               >  1:1146607/1‑137 (MQ=255)

                         cGCAATAATGTCTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAAcg                                                                                                               <  2:1146607/137‑1 (MQ=255)

                           cAATAATGTCTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATGTGGTGCTTTTTCAACAAcgcgcg                                                                                                           <  2:1271378/139‑1 (MQ=255)

                                   tCTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACtgat                                                                                                   >  2:130423/1‑139 (MQ=255)

                                      gATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTgatgat                                                                                                >  2:1328694/1‑139 (MQ=255)

                                           gctgcgctTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGtt                                                                                           <  1:385407/139‑1 (MQ=255)

                                                     tCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTa                                                                                 >  2:586703/1‑139 (MQ=255)

                                                            tGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGaa                                                                                                        >  2:804082/1‑109 (MQ=255)

                                                            tGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGaa                                                                                                        <  1:804082/109‑1 (MQ=255)

                                                              cAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCcg                                                                        >  2:165933/1‑139 (MQ=255)

                                                              cAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCcg                                                                        >  1:1074724/1‑139 (MQ=255)

                                                                aaCGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCcgcg                                                                      <  2:29032/139‑1 (MQ=255)

                                                                aaCGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCcgcg                                                                      <  1:586703/139‑1 (MQ=255)

                                                                    acgTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCAGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCt                                                                  <  1:1260770/139‑1 (MQ=255)

                                                                         ttGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTgg                                                             <  2:1074724/139‑1 (MQ=255)

                                                                               atgtatgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGaa                                                                                                        <  2:162074/90‑1 (MQ=255)

                                                                               atgtatgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGaa                                                                                                        >  1:162074/1‑90 (MQ=255)

                                                                               atgtatgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAg                                                                                             >  2:345293/1‑101 (MQ=255)

                                                                               atgtatgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAg                                                                                             <  1:345293/101‑1 (MQ=255)

                                                                                 gtatgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGt                                                                                                                              >  1:628620/1‑66 (MQ=255)

                                                                                 gtatgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGt                                                                                                                              <  2:628620/66‑1 (MQ=255)

                                                                                   atgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTcc                                                   >  1:77317/1‑139 (MQ=255)

                                                                                    tgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTcaaca                                                                                                                  <  2:825144/75‑1 (MQ=255)

                                                                                    tgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTcaaca                                                                                                                  >  1:825144/1‑75 (MQ=255)

                                                                                    tgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGaa                                                                                                        <  2:197266/85‑1 (MQ=255)

                                                                                    tgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGaa                                                                                                        >  1:197266/1‑85 (MQ=255)

                                                                                    tgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTccc                                                  >  2:1066478/1‑139 (MQ=255)

                                                                                    tgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTccc                                                  <  1:886723/139‑1 (MQ=255)

                                                                                      ccGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAAcgcgcg                                                                                                           >  2:3939/1‑80 (MQ=255)

                                                                                      ccGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAAcgcgcg                                                                                                           <  1:3939/80‑1 (MQ=255)

                                                                                      ccGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAAcg                                                                                                               >  2:63843/1‑76 (MQ=255)

                                                                                      ccGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAAcg                                                                                                               <  1:63843/76‑1 (MQ=255)

                                                                                      ccGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTc                                                                >  2:606696/1‑123 (MQ=255)

                                                                                      ccGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTc                                                                <  1:606696/123‑1 (MQ=255)

                                                                                      ccGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTgg                                                             >  1:503567/1‑126 (MQ=255)

                                                                                      ccGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTgg                                                             <  2:503567/126‑1 (MQ=255)

                                                                                      ccGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTg                                                              <  1:1346855/125‑1 (MQ=255)

                                                                                      ccGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTg                                                              >  2:1346855/1‑125 (MQ=255)

                                                                                       cGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAAcgcg                                                                                                             >  1:236018/1‑77 (MQ=255)

                                                                                       cGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAAcgcg                                                                                                             <  2:236018/77‑1 (MQ=255)

                                                                                          aTAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAAcgcgcg                                                                                                           >  1:26179/1‑76 (MQ=255)

                                                                                          aTAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAAcgcgcg                                                                                                           <  2:26179/76‑1 (MQ=255)

                                                                                          aTAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCtt                                                                 >  2:764806/1‑118 (MQ=255)

                                                                                          aTAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCtt                                                                 <  1:764806/118‑1 (MQ=255)

                                                                                          aTAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTta                                                          >  1:241647/1‑125 (MQ=255)

                                                                                          aTAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTta                                                          <  2:241647/125‑1 (MQ=255)

                                                                                            aaaGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCa                                          <  1:25953/139‑1 (MQ=255)

                                                                                              aGCTTTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCaaa                                        <  1:1381179/139‑1 (MQ=255)

                                                                                                       gccgcATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCcgcgcg                                                                    <  2:728270/102‑1 (MQ=255)

                                                                                                       gccgcATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCcgcgcg                                                                    >  1:728270/1‑102 (MQ=255)

                                                                                                         cgcATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCt                                                                  <  1:1306067/102‑1 (MQ=255)

                                                                                                         cgcATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCt                                                                  >  2:1306067/1‑102 (MQ=255)

                                                                                                           cATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTgcagca                           >  1:970136/1‑139 (MQ=255)

                                                                                                           cATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTgcagca                           <  1:521490/139‑1 (MQ=255)

                                                                                                            aTCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCaa                          <  1:1328694/139‑1 (MQ=255)

                                                                                                             tCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAAc                         >  1:1430033/1‑139 (MQ=255)

                                                                                                                 gCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCtt                     <  1:165933/139‑1 (MQ=255)

                                                                                                                      ttGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTgcagca                           >  2:1268087/1‑128 (MQ=255)

                                                                                                                      ttGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTgcagca                           <  1:1268087/128‑1 (MQ=255)

                                                                                                                              cTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGtt                                                           >  1:806504/1‑88 (MQ=255)

                                                                                                                              cTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGtt                                                           <  2:806504/88‑1 (MQ=255)

                                                                                                                              cTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTgcagc                            <  2:544399/119‑1 (MQ=255)

                                                                                                                              cTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTgcagc                            >  1:544399/1‑119 (MQ=255)

                                                                                                                              cTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCAt        >  1:658346/1‑139 (MQ=255)

                                                                                                                              cTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCAt        >  2:787544/1‑139 (MQ=255)

                                                                                                                                   cTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGaa   >  2:718400/1‑139 (MQ=255)

                                                                                                                                    ttCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGaaa  <  2:77317/139‑1 (MQ=255)

ATGCGCTGAGCGATTAATAGCCCAACGCAATAATGTCTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGCTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTGAAA  >  NC_000913/1366799‑1367069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A