Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I2 R1	758	59.3	2864096	93.9%	2689386	105.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,729,692	G→A	A202A (GCG→GCA)	lhr →	putative ATP‑dependent helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,729,692	0	G	A	100.0%	104.0 / NA	33	A202A (GCG→GCA)	lhr	putative ATP‑dependent helicase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/17); total (16/17)

CTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCTGCCACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGA  >  NC_000913/1729558‑1729822

ctctATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCactgc                                                                                                                                >  2:807933/1‑139 (MQ=255)

  ctATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGTCGTTAAGTCTGGAGCGGCTCGATGCACTGCTc                                                                                                                              <  2:850353/139‑1 (MQ=255)

    aTCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCACTGCTCca                                                                                                                            <  2:307913/139‑1 (MQ=255)

    aTCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCACTGCTCca                                                                                                                            <  2:600776/139‑1 (MQ=255)

         aTGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCACTGCTCCACACCt                                                                                                                       <  1:508210/139‑1 (MQ=255)

           gCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCACTGCTCCACACCTCa                                                                                                                     >  1:1143548/1‑139 (MQ=255)

           gCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCACTGCTCCACACCTCa                                                                                                                     >  1:1243138/1‑139 (MQ=255)

               aCCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCACTGCTCCACACCTCAGcac                                                                                                                 >  1:60081/1‑139 (MQ=255)

                          gcgcgAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCACTGCTCCACACCTCAGCACAGCGAATTGGc                                                                                                      <  1:1207915/139‑1 (MQ=255)

                            gcgAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCACTGCTCCACACCTCAGCACAGCGAATTGGCCt                                                                                                    <  2:60081/139‑1 (MQ=255)

                                aaCGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCACTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCt                                                                                                <  2:778789/139‑1 (MQ=255)

                                  cGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCACTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCTGc                                                                                              <  1:807933/139‑1 (MQ=255)

                                  cGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCACTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCTGc                                                                                              <  2:1135218/139‑1 (MQ=255)

                                       cgcgGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCACTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCTGCCACtg                                                                                         >  2:837619/1‑139 (MQ=255)

                                                                atgaAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCACTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCTGCCACTGTgcgc                                                                                    <  1:1426955/119‑1 (MQ=255)

                                                                atgaAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCACTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCTGCCACTGTgcgc                                                                                    >  2:1426955/1‑119 (MQ=255)

                                                                     ttCCACGCGGTAGCGGGCGGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCACTGCTCCACACCTCAGCACAGCGAGTTg                                                                                                        <  2:920934/93‑1 (MQ=255)

                                                                     gTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCACTGCTCCACACCTCAGCACAGCGAATTg                                                                                                        >  1:920934/1‑94 (MQ=255)

                                                                     gTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCACTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCTGCCACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTc                                                           >  1:1191140/1‑139 (MQ=255)

                                                                     gTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCACTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCTGCCACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTc                                                           >  1:1196260/1‑139 (MQ=255)

                                                                        cACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCACTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCTGCCACTGTGCGCTCAGCCAGCGAtgt                                                                      <  2:1206102/125‑1 (MQ=255)

                                                                        cACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCACTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCTGCCACTGTGCGCTCAGCCAGCGAtgt                                                                      >  1:1206102/1‑125 (MQ=255)

                                                                                       aGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCACTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCTGCCACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCgg                                         >  2:255143/1‑139 (MQ=255)

                                                                                         tAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCACTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCTGCCACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGc                                             <  2:1320741/133‑1 (MQ=255)

                                                                                         tAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCACTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCTGCCACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGc                                             >  1:1320741/1‑133 (MQ=255)

                                                                                           aaCGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCACTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCTGCCACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTAc                                     <  2:1143548/139‑1 (MQ=255)

                                                                                                   gcgcATCTGGCGTTAAGTCTGGAGCGGCTCGATGCACTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCTGCCACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCa                             >  2:675449/1‑139 (MQ=255)

                                                                                                    cgcATCTGGCGTTAAGTCTGGAGCGGCTCGATGCACTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCTGCCACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCaa                            <  2:1243138/139‑1 (MQ=255)

                                                                                                      cATCTGGCGTTAAGTCTGGAGCGGCTCGATGCACTGCTCCACACCTCAGCACAGCGAATTGGCCATTCTGCCACTGTGCGCTCAGCCAGCGATGTGgc                                                                   <  2:564780/98‑1 (MQ=255)

                                                                                                      cATCTGGCGTTAAGTCTGGAGCGGCTCGATGCACTGCTCCACACCTCAGCACAGCGAATTGGCCATTCTGCCACTGTGCGCTCAGCCAGCGATGTGgc                                                                   >  1:564780/1‑98 (MQ=255)

                                                                                                            gCGTTAAGTCTGGAGCGGCTCGATGCACTGCTCCACACCTCAGCACAGCGAATTGGc                                                                                                      <  1:782360/57‑1 (MQ=255)

                                                                                                            gCGTTAAGTCTGGAGCGGCTCGATGCACTGCTCCACACCTCAGCACAGCGAATTGGc                                                                                                      >  2:782360/1‑57 (MQ=255)

                                                                                                                      tGGAGCGGCTCGATGCACTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCTGCCACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCa          >  2:610603/1‑139 (MQ=255)

                                                                                                                              cTCGATGCACTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCTGCCACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGa  >  1:897151/1‑139 (MQ=255)

CTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCTGCTCCACACCTCAGCACAGCGAATTGGCCTTTCTGCCACTGTGCGCTCAGCCAGCGATGTGGCAGCATTTCTTGGTGGCGATCGCCCGGTTACGGTAGTCAACCCGCCCGCAATGCGCCATCCGCAGA  >  NC_000913/1729558‑1729822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A