Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I2 R1	758	59.3	2864096	93.9%	2689386	105.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,796,337	C→T	150 (TAG→TAA)	ygaU ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,796,337	0	C	T	100.0%	151.8 / NA	48	150 (TAG→TAA)	ygaU	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (23/25); total (23/25)

GGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCA  >  NC_000913/2796201‑2796467

ggaggaTAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAattaatta                                                                                                                                  >  2:1001088/1‑139 (MQ=255)

          cacGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGa                                                                                                                        <  1:1001088/139‑1 (MQ=255)

            cGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGa                                                                                                                        >  1:860035/1‑137 (MQ=255)

            cGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGa                                                                                                                        <  2:860035/137‑1 (MQ=255)

               tCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACg                                                                                                                   <  1:310053/139‑1 (MQ=255)

                       aGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATa                                                                                                                     <  2:781567/129‑1 (MQ=255)

                       aGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATa                                                                                                                     >  1:781567/1‑129 (MQ=255)

                         aCCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAattaatta                                                                                                                                  <  1:973658/114‑1 (MQ=255)

                         aCCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGc                                                                                                         >  2:674499/1‑139 (MQ=255)

                                cTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGAt                                                                                                  >  2:235818/1‑139 (MQ=255)

                                   cggcggTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATaaa                                                                                               >  1:905460/1‑139 (MQ=255)

                                            tGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCg                                                                                      <  1:235818/139‑1 (MQ=255)

                                              gcgcGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAattaatta                                                                                                                                  >  2:88433/1‑93 (MQ=255)

                                                   agaTACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAattact                                                                                                                                <  2:808481/90‑1 (MQ=255)

                                                   agaTACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAattact                                                                                                                                >  1:808481/1‑90 (MQ=255)

                                                   agaTACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCTGAATACGCAACACTTGCCCCGGATAAATTTTATCCg                                                                                      <  2:1030152/132‑1 (MQ=255)

                                                   agaTACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCg                                                                                      >  1:1030152/1‑132 (MQ=255)

                                                                gcCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTa                                                                  >  1:818756/1‑139 (MQ=255)

                                                                            aTGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCa                                                                          <  2:1130198/119‑1 (MQ=255)

                                                                            aTGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCa                                                                          >  1:1130198/1‑119 (MQ=255)

                                                                               cgcCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGc                                                                                                                  >  1:570774/1‑76 (MQ=255)

                                                                               cgcCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGc                                                                                                                  <  2:570774/76‑1 (MQ=255)

                                                                               cgcCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGAtt                                                   >  1:1072324/1‑139 (MQ=255)

                                                                                 ccAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAacac                                                                                                             >  1:235339/1‑79 (MQ=255)

                                                                                 ccAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAacac                                                                                                             <  2:235339/79‑1 (MQ=255)

                                                                                  cAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTa                                                <  1:674499/139‑1 (MQ=255)

                                                                                  cAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTa                                                <  2:905460/139‑1 (MQ=255)

                                                                                   aGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAattaatta                                                                                                                                  <  1:45371/56‑1 (MQ=255)

                                                                                   aGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAattaatta                                                                                                                                  >  2:45371/1‑56 (MQ=255)

                                                                                          aCTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCTAAGATTTTATTGTACAg                                        <  1:895267/139‑1 (MQ=255)

                                                                                          aCTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAg                                        >  1:168540/1‑139 (MQ=255)

                                                                                             gACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGAtt                                     <  2:168540/139‑1 (MQ=255)

                                                                                              aCGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGAt                                      >  2:721898/1‑137 (MQ=255)

                                                                                              aCGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGAt                                      <  1:721898/137‑1 (MQ=255)

                                                                                                gCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGc                                  >  1:250028/1‑139 (MQ=255)

                                                                                                  ccaTTCCCATTATCCCGCAGGCGATGGCTGGCAATTAATTACTCTTCCGCAACAGGTAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGCTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGt                                <  2:963775/137‑1 (MQ=255)

                                                                                                            tATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGGTTAGCGTTACCGTAGAc                      <  2:818756/139‑1 (MQ=255)

                                                                                                            tATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTa                              >  2:319213/1‑131 (MQ=255)

                                                                                                            tATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTa                              <  1:319213/131‑1 (MQ=255)

                                                                                                            tATCCAGCAGGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGAc                      <  2:1072324/139‑1 (MQ=255)

                                                                                                                cagcagGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATcc                                                                                       >  2:734429/1‑70 (MQ=255)

                                                                                                                cagcagGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATcc                                                                                       <  1:734429/70‑1 (MQ=255)

                                                                                                                  gcagGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATcc                                                                                       >  2:1323211/1‑68 (MQ=255)

                                                                                                                  gcagGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATcc                                                                                       <  1:1323211/68‑1 (MQ=255)

                                                                                                                   cagGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAAtt                                                                                             <  2:930014/61‑1 (MQ=255)

                                                                                                                   cagGCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAAtt                                                                                             >  1:930014/1‑61 (MQ=255)

                                                                                                                     ggCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGt                                >  1:812379/1‑120 (MQ=255)

                                                                                                                     ggCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGt                                <  2:812379/120‑1 (MQ=255)

                                                                                                                      gCGATGGCTGGCAATTAATTACTCTTCCGGAATACGTAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGAt                                      <  2:1025871/113‑1 (MQ=255)

                                                                                                                      gCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGAt                                      >  1:1025871/1‑113 (MQ=255)

                                                                                                                      gCGATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGaa            <  2:250028/139‑1 (MQ=255)

                                                                                                                        gATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCtttt                                                                               >  1:505893/1‑70 (MQ=255)

                                                                                                                        gATGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCtttt                                                                               <  2:505893/70‑1 (MQ=255)

                                                                                                                         aTGGCTGGCAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATg         >  1:33130/1‑139 (MQ=255)

                                                                                                                                gcAATTAATTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCa  >  2:403002/1‑139 (MQ=255)

GGAGGATAAACACGATCAGCACCAGACCCTGACTGCGGCGGTACTGGCGCGAGATACCGCGCGCGCCAGTGAGTTAATGCGCCAGCATTTACTGACGCCAATTCCCATTATCCAGCAGGCGATGGCTGGCAATTAACTACTCTTCCGGAATACGCAACACTTGCCCCGGATAAATTTTATCCGGGCTTTTTAGCATCGGTTTATTCGCTTCGAAGATTTTATTGTACAGATTAGCGTTACCGTAGACCTGTTTGGAAATGGCACTCA  >  NC_000913/2796201‑2796467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A