Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I2 R1	758	59.3	2864096	93.9%	2689386	105.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,817,271	G→A	A78V (GCA→GTA)	yqaB ←	fructose‑1‑P and 6‑phosphogluconate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,817,271	0	G	A	100.0%	146.2 / NA	46	A78V (GCA→GTA)	yqaB	fructose‑1‑P and 6‑phosphogluconate phosphatase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (25/21); total (25/21)

CCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGT  >  NC_000913/2817143‑2817406

ccAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGtttt                                                                                                                               >  1:1320299/1‑139 (MQ=255)

   gCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTc                                                                                                                            <  2:731384/139‑1 (MQ=255)

      aTGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCAcg                                                                                                                         >  2:104277/1‑139 (MQ=255)

                  tGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGCGGAAGCGGTTCGACGCTATCCATCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATg                                                                                                             <  1:632978/139‑1 (MQ=255)

                  tGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCAcgcg                                                                                                                       <  2:1329186/129‑1 (MQ=255)

                  tGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCAcgcg                                                                                                                       >  1:1329186/1‑129 (MQ=255)

                  tGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATg                                                                                                             <  2:1320299/139‑1 (MQ=255)

                            aCTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCgaga                                                                                                   <  1:882887/139‑1 (MQ=255)

                             cTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGAt                                                                                                  <  1:73404/139‑1 (MQ=255)

                                  cGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGc                                                                                              >  1:57901/1‑138 (MQ=255)

                                  cGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGc                                                                                              <  2:57901/138‑1 (MQ=255)

                                            gCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCt                                                                                   <  1:1174841/139‑1 (MQ=255)

                                                      gacgacCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGc                                                                         <  2:1117882/139‑1 (MQ=255)

                                                              tGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCaa                                                                 >  1:507331/1‑139 (MQ=255)

                                                                               aTCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGAt                                                >  2:1132369/1‑139 (MQ=255)

                                                                                        agGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAgc                                       >  1:257980/1‑139 (MQ=255)

                                                                                         ggAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCa                                                                                                                           <  1:546753/54‑1 (MQ=255)

                                                                                         ggAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCa                                                                                                                           >  2:546753/1‑54 (MQ=255)

                                                                                         ggAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAgcg                                      >  1:411311/1‑139 (MQ=255)

                                                                                          gAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAg                                                                    >  2:555874/1‑108 (MQ=255)

                                                                                          gAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAg                                                                    <  1:555874/108‑1 (MQ=255)

                                                                                          gAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAgcgc                                     >  2:731897/1‑139 (MQ=255)

                                                                                            aGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCATGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCaa                                   >  2:889560/1‑139 (MQ=255)

                                                                                            aGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGAtt                                                                                        >  1:739651/1‑86 (MQ=255)

                                                                                            aGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGAtt                                                                                        <  2:739651/86‑1 (MQ=255)

                                                                                            aGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCaa                                   >  1:604193/1‑139 (MQ=255)

                                                                                            aGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCaa                                   >  2:1092727/1‑139 (MQ=255)

                                                                                            aGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCaa                                   >  1:1375383/1‑139 (MQ=255)

                                                                                               ggTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGAt                                                                                                  >  1:591680/1‑73 (MQ=255)

                                                                                               ggTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGAt                                                                                                  <  2:591680/73‑1 (MQ=255)

                                                                                                gTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCATGCGCTAACGCATGCGGGTCGAGTTCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAatcat                               <  1:889560/139‑1 (MQ=255)

                                                                                                gTTCGACGCTATCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAatcat                               <  2:507331/139‑1 (MQ=255)

                                                                                                          aTCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAata                     <  1:1092727/139‑1 (MQ=255)

                                                                                                           tCCAGCAGCATACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAatat                    >  2:741045/1‑139 (MQ=255)

                                                                                                                cagcaTACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTAc               >  2:830335/1‑139 (MQ=255)

                                                                                                                 agcaTACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATg                                                                                                             <  2:245806/44‑1 (MQ=255)

                                                                                                                 agcaTACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATg                                                                                                             >  1:245806/1‑44 (MQ=255)

                                                                                                                     tACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGcc                                                                                             <  1:684789/56‑1 (MQ=255)

                                                                                                                     tACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGcc                                                                                             >  2:684789/1‑56 (MQ=255)

                                                                                                                     tACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGTGCGATCCATTAAGCGCAATCATCGCCTGAATATc                   <  2:943069/130‑1 (MQ=255)

                                                                                                                     tACTTCTTACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATc                   >  1:943069/1‑130 (MQ=255)

                                                                                                                        ttcttACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTgg                                                     <  2:618068/93‑1 (MQ=255)

                                                                                                                        ttcttACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTgg                                                     >  1:618068/1‑93 (MQ=255)

                                                                                                                        ttcttACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGAcc       >  1:1034075/1‑139 (MQ=255)

                                                                                                                          cttACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCg                                                  <  2:111364/94‑1 (MQ=255)

                                                                                                                          cttACTACTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCg                                                  >  1:111364/1‑94 (MQ=255)

                                                                                                                            tactacTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAg   <  1:741045/139‑1 (MQ=255)

                                                                                                                             actactTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGt  >  2:669889/1‑139 (MQ=255)

                                                                                                                             actactTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGt  >  2:246954/1‑139 (MQ=255)

CCAGCAATGCCTCAGCGATGGCGCTTTCACTCCCCGTTCCTACAGCCATTGGGCGACGACCATGCCAACTTTTCACCACATCAACAAGAGGAAGCGGTTCGACGCTATCCAGCAGCATACTTCTTACTGCTTCTGTTTTTTCACGCGCTAACGCATGCGGGTCGAGATCGGCCTGATTCAGCTCAATAATTGCCTGAGCAATACGCCAGGTGGGCGATCCATTAAGCGCAATCATCGCCTGAATATCGTACTGAAGACCGTAGT  >  NC_000913/2817143‑2817406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A