Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I2 R1 758 59.3 2864096 93.9% 2689386 105.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,336,405 A→G S209S (AGT→AGC)  adiC ← arginine:agmatine antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,336,4050AG100.0% 178.2 / NA 50S209S (AGT→AGC) adiCarginine:agmatine antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/22);  total (28/22)

GGGATCATCCCCATAATCGCGGTGGTAGAAAGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAAC  >  NC_000913/4336280‑4336516
                                                                                                                             |                                                                                                               
gggATCATCCCCATAATCGCGGTGGTAGAAAGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGa                                                                                                    >  1:750258/1‑139 (MQ=255)
     catcCCCATAATCGCGGTGGTAGAAAGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAAc                                                                                               >  1:1096756/1‑139 (MQ=255)
       tcCCCATAATCGCGGTGGTAGAAAGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGaa                                                                                                <  2:327231/136‑1 (MQ=255)
       tcCCCATAATCGCGGTGGTAGAAAGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGaa                                                                                                >  1:327231/1‑136 (MQ=255)
       tcCCCATAATCGCGGTGGTAGAAAGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGa                                                                                             >  2:798315/1‑139 (MQ=255)
       tcCCCATAATCGCGGTGGTAGAAAGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGa                                                                                             >  1:302291/1‑139 (MQ=255)
        ccccATAATCGCGGTGGTAGAAAGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGAc                                                                                            >  1:32988/1‑139 (MQ=255)
                tCGCGGTGGTAGAAAGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGt                                                                                    <  1:566625/139‑1 (MQ=255)
                            aaaGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGt                                                                        <  2:1127724/139‑1 (MQ=255)
                                tACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCg                                                                                     >  2:689819/1‑122 (MQ=255)
                                tACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCg                                                                                     <  1:689819/122‑1 (MQ=255)
                                     aGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAAt                                                               >  1:1086345/1‑139 (MQ=255)
                                       cAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACGCCGATGAACGACCCCAGCGTAACGTTAAGGGTACTTTGAATTg                                                             >  2:928120/1‑139 (MQ=255)
                                       cAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGa                                                                                                    >  2:181674/1‑100 (MQ=255)
                                       cAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGa                                                                                                    <  1:181674/100‑1 (MQ=255)
                                          aCGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTcc                                                                                                           >  1:60458/1‑90 (MQ=255)
                                          aCGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTcc                                                                                                           <  2:60458/90‑1 (MQ=255)
                                          aCGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAgg                                                                          <  2:1148778/123‑1 (MQ=255)
                                          aCGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAgg                                                                          >  1:1148778/1‑123 (MQ=255)
                                             gcggcAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGa                                                       <  2:1086345/139‑1 (MQ=255)
                                                             caccaATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCt                                       >  2:1048005/1‑139 (MQ=255)
                                                              accaATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAAc                                                                                               >  2:476251/1‑82 (MQ=255)
                                                              accaATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAAc                                                                                               <  1:476251/82‑1 (MQ=255)
                                                              accaATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGt                                                                                    >  2:573122/1‑93 (MQ=255)
                                                              accaATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGt                                                                                    <  1:573122/93‑1 (MQ=255)
                                                              accaATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAgg                                                                          <  2:45739/103‑1 (MQ=255)
                                                              accaATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAgg                                                                          >  1:45739/1‑103 (MQ=255)
                                                              accaATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTg                                      >  1:896430/1‑139 (MQ=255)
                                                              accaATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTg                                      <  1:1233404/139‑1 (MQ=255)
                                                                  aTGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGt                                  >  2:722871/1‑139 (MQ=255)
                                                                     gtggCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGc                                           >  1:799721/1‑127 (MQ=255)
                                                                     gtggCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGc                                           <  2:799721/127‑1 (MQ=255)
                                                                           aTAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATgccg                         <  1:1007557/139‑1 (MQ=255)
                                                                             aGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAg                                             <  1:348104/117‑1 (MQ=255)
                                                                             aGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAg                                             >  2:348104/1‑117 (MQ=255)
                                                                                gACATTGCGTTTCGGGTTTTTCACTACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCata                    <  1:798315/139‑1 (MQ=255)
                                                                                     tGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCt                                                                                                               >  1:280858/1‑43 (MQ=255)
                                                                                     tGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCca                                                                                                          >  1:943967/1‑48 (MQ=255)
                                                                                     tGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCca                                                                                                          <  2:943967/48‑1 (MQ=255)
                                                                                         tttCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAggg                                                                         >  1:1046853/1‑77 (MQ=255)
                                                                                         tttCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAggg                                                                         <  2:1046853/77‑1 (MQ=255)
                                                                                         tttCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTca           >  1:867095/1‑139 (MQ=255)
                                                                                                 ttttCACCACACCTGCGGCAACGGAGGCGCTTTCca                                                                                                          >  2:221376/1‑36 (MQ=255)
                                                                                                 ttttCACCACACCTGCGGCAACGGAGGCGCTTTCca                                                                                                          <  1:221376/36‑1 (MQ=255)
                                                                                                         acacCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGgaac  >  2:472185/1‑132 (MQ=255)
                                                                                                         acacCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGgaac  <  1:472185/132‑1 (MQ=255)
                                                                                                          cacCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATgccgcc                       <  2:1232691/110‑1 (MQ=255)
                                                                                                          cacCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATgccgcc                       >  1:1232691/1‑110 (MQ=255)
                                                                                                                cGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAgg                                                                          >  2:268757/1‑53 (MQ=255)
                                                                                                                cGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAgg                                                                          <  1:268757/53‑1 (MQ=255)
                                                                                                                             |                                                                                                               
GGGATCATCCCCATAATCGCGGTGGTAGAAAGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAAC  >  NC_000913/4336280‑4336516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A