Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I2 R1	765	53.2	2691310	93.4%	2513683	103.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,336,405	A→G	S209S (AGT→AGC)	adiC ←	arginine:agmatine antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,336,405	0	A	G	100.0%	227.4 / NA	63	S209S (AGT→AGC)	adiC	arginine:agmatine antiporter
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (31/32); total (31/32)

TGCGGCATTAGGGATCATCCCCATAATCGCGGTGGTAGAAAGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCCAAATACGGCA  >  NC_000913/4336270‑4336537

tGCGGCATTAGGGATCATCCCCATAATCGCGGTGGTAGAAAGTACATAGCAAACGGCGGCAATCAGTACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCt                                                                                                                                    <  1:732379/138‑1 (MQ=255)

  cGGCATTAGGGATCATCCCCATAATCGCGGTGGTAGAAAGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTc                                                                                                                                 >  2:626823/1‑139 (MQ=255)

        tAGGGATCATCCCCATAATCGCGGTGGTAGAAAGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACAcc                                                                                                                           <  2:407832/139‑1 (MQ=255)

                   cccATAATCGCGGTGGTAGAAAGTACATAGCAAACGGCGGCAATAAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGAcc                                                                                                                <  2:340143/139‑1 (MQ=255)

                          tCGCGGTGGTAGAAAGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGt                                                                                                         <  1:626823/139‑1 (MQ=255)

                               gtggtAGAAAGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGTGGCGCTTTCCACACCGa                                                                                                                         <  2:565343/118‑1 (MQ=255)

                               gtggtAGAAAGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGa                                                                                                                         >  1:565343/1‑118 (MQ=255)

                                            cATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTg                                                                                       >  2:1157827/1‑139 (MQ=255)

                                            cATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTg                                                                                       >  2:1191730/1‑139 (MQ=255)

                                            cATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTg                                                                                       >  2:185821/1‑139 (MQ=255)

                                               aGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAAt                                                                                    >  1:777659/1‑139 (MQ=255)

                                                 cAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAgg                                                                                               <  2:1289360/126‑1 (MQ=255)

                                                 cAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAgg                                                                                               >  1:1289360/1‑126 (MQ=255)

                                                   aaCGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCa                                                                                <  2:64567/139‑1 (MQ=255)

                                                    aCGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGt                                                                                                    <  2:668267/118‑1 (MQ=255)

                                                    aCGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGt                                                                                                    >  1:668267/1‑118 (MQ=255)

                                                         ggcAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGa                                                                                                                         <  2:23534/92‑1 (MQ=255)

                                                         ggcAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGa                                                                                                                         >  1:23534/1‑92 (MQ=255)

                                                           caatcaatACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACt                                                                                          >  1:1175050/1‑121 (MQ=255)

                                                           caatcaatACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACt                                                                                          <  2:1175050/121‑1 (MQ=255)

                                                                       caccaATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAgg                                                                                               <  2:275172/104‑1 (MQ=255)

                                                                       caccaATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAgg                                                                                               >  1:275172/1‑104 (MQ=255)

                                                                        accaATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAgg                                                                                               <  1:433004/103‑1 (MQ=255)

                                                                        accaATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAgg                                                                                               >  2:433004/1‑103 (MQ=255)

                                                                         ccaATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACca                                                                                                               >  1:619473/1‑86 (MQ=255)

                                                                         ccaATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACca                                                                                                               <  2:619473/86‑1 (MQ=255)

                                                                         ccaATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGa                                                          >  2:122389/1‑139 (MQ=255)

                                                                              ggtggCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAg                                                                          >  1:506804/1‑118 (MQ=255)

                                                                              ggtggCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAg                                                                          <  2:506804/118‑1 (MQ=255)

                                                                               gtggCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTcc                                                    >  1:722176/1‑139 (MQ=255)

                                                                                 ggCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCAt                                                  <  1:1191730/139‑1 (MQ=255)

                                                                                  gCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATg                                                 <  1:1157827/139‑1 (MQ=255)

                                                                                   cGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAg                                                                          <  2:546109/113‑1 (MQ=255)

                                                                                   cGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAg                                                                          >  1:546109/1‑113 (MQ=255)

                                                                                    gATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATgcc                                               >  2:696293/1‑139 (MQ=255)

                                                                                    gATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATgcc                                               <  1:122389/139‑1 (MQ=255)

                                                                                     aTAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAAc                                                                                                      <  2:1265194/83‑1 (MQ=255)

                                                                                     aTAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAAc                                                                                                      >  1:1265194/1‑83 (MQ=255)

                                                                                     aTAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATgccg                                              <  1:185821/139‑1 (MQ=255)

                                                                                     aTAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATgccg                                              <  2:777659/139‑1 (MQ=255)

                                                                                     aTAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATgccg                                              >  1:749779/1‑139 (MQ=255)

                                                                                     aTAGGGACATTGCGTTTCGGGTGTTTCACCACACCTCCGGCAACAGAGGCGCTTGCCACCCCGATGAGCGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATgccg                                              <  2:1344070/139‑1 (MQ=255)

                                                                                       aGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTcc                                                                                                                                <  2:769108/55‑1 (MQ=255)

                                                                                       aGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTcc                                                                                                                                >  1:769108/1‑55 (MQ=255)

                                                                                         ggACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGc                                                                                                           <  1:347550/74‑1 (MQ=255)

                                                                                         ggACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGc                                                                                                           >  2:347550/1‑74 (MQ=255)

                                                                                         ggACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCat                                          >  1:58745/1‑139 (MQ=255)

                                                                                            cATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCAtata                                       >  2:767803/1‑139 (MQ=255)

                                                                                               tGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGt                                                                                                         >  2:1281178/1‑70 (MQ=255)

                                                                                               tGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGt                                                                                                         <  1:1281178/70‑1 (MQ=255)

                                                                                               tGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATgccg                                              >  1:1046082/1‑129 (MQ=255)

                                                                                               tGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATgccg                                              <  2:1046082/129‑1 (MQ=255)

                                                                                                    ttCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGt                                                                                                         <  1:400686/65‑1 (MQ=255)

                                                                                                    ttCGGGTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGt                                                                                                         >  2:400686/1‑65 (MQ=255)

                                                                                                       gggTTTTTCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTcaccac                            <  2:684187/139‑1 (MQ=255)

                                                                                                            tttCACCACACCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGtctgtctcttatac                       <  2:722176/139‑14 (MQ=255)

                                                                                                                   acacCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATgc                                                <  2:990455/107‑1 (MQ=255)

                                                                                                                   acacCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATgc                                                >  1:990455/1‑107 (MQ=255)

                                                                                                                     acCTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGaaccagaaccag              >  2:396051/1‑139 (MQ=255)

                                                                                                                       cTGCGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGcc            >  2:1339842/1‑139 (MQ=255)

                                                                                                                          cGGCAACGGAGGCGCTTTCCAGACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAgg                                                                 >  2:809648/1‑83 (MQ=255)

                                                                                                                          cGGCAACGGAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAgg                                                                 <  1:809648/83‑1 (MQ=255)

                                                                                                                                 ggAGGCGCTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCCAAATACGGCa  <  1:696293/139‑1 (MQ=255)

TGCGGCATTAGGGATCATCCCCATAATCGCGGTGGTAGAAAGTACATAGCAAACGGCGGCAATCAATACCCCACCAATGGTGGCGATAGGGACATTGCGTTTCGGGTTTTTCACCACACCTGCGGCAACGGAGGCACTTTCCACACCGATGAACGACCACAGCGTAACGTTAAGGGTACTTTGAATTGCACCGAAGGTGCCCAGGCCGCTGACGTTCCATGCCGCCATATAGGTTTCACCACGGAACCAGAACCAGCCAAATACGGCA  >  NC_000913/4336270‑4336537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A