Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I2 R1	758	59.3	2864096	93.9%	2689386	105.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,614,260	G→A	260 (TAG→TAA)	yjjV →	putative DNase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,614,260	0	G	A	100.0%	97.4 / NA	31	260 (TAG→TAA)	yjjV	putative DNase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/14); total (17/14)

GTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGT  >  NC_000913/4614163‑4614394

gtgtgtTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGcac                                                                                               <  2:467612/139‑1 (MQ=255)

            tGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCAcgcc                                                                                                                   <  2:870635/107‑1 (MQ=255)

            tGCTTTGCGAGTTGAGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTGTAACGTGCCGTAAGCCGGATAAGGCGTTCAcgcc                                                                                                                   >  1:870635/1‑107 (MQ=255)

             gCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCAcgccgc                                                                                                                 >  2:503010/1‑108 (MQ=255)

             gCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCAcgccgc                                                                                                                 <  1:503010/108‑1 (MQ=255)

                     aGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTaa                                                                          <  1:473076/139‑1 (MQ=255)

                                           tGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAgg                                                    >  2:233562/1‑139 (MQ=255)

                                                    gcAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTaa                                                                          <  2:868040/108‑1 (MQ=255)

                                                    gcAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTaa                                                                          >  1:868040/1‑108 (MQ=255)

                                                       aGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGcaca                                                                                              >  2:407995/1‑85 (MQ=255)

                                                       aGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGcaca                                                                                              <  1:407995/85‑1 (MQ=255)

                                                                      cacGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAgg                                  <  2:183965/130‑1 (MQ=255)

                                                                      cacGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAgg                                  >  1:183965/1‑130 (MQ=255)

                                                                        cGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGgcg                                                                                                  <  1:382441/64‑1 (MQ=255)

                                                                        cGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGgcg                                                                                                  >  2:382441/1‑64 (MQ=255)

                                                                        cGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCa                                                              >  1:30436/1‑100 (MQ=255)

                                                                        cGTATACGTTGTTTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCa                                                              <  2:30436/100‑1 (MQ=255)

                                                                                 tgttTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTc                                                                    >  2:1307038/1‑85 (MQ=255)

                                                                                 tgttTAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTc                                                                    <  1:1307038/85‑1 (MQ=255)

                                                                                   tttAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTtgtg                                              >  1:488845/1‑105 (MQ=255)

                                                                                   tttAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTtgtg                                              <  2:488845/105‑1 (MQ=255)

                                                                                     tAACGTGCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCAt          >  2:571771/1‑139 (MQ=255)

                                                                                           gCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTAc                                                       >  2:726462/1‑88 (MQ=255)

                                                                                           gCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTAc                                                       <  1:726462/88‑1 (MQ=255)

                                                                                           gCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa    >  2:347398/1‑139 (MQ=255)

                                                                                           gCCGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa    >  1:738534/1‑139 (MQ=255)

                                                                                             cGTAAGCCGGATAAGGTGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGTGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGcc                                            <  1:898639/97‑1 (MQ=255)

                                                                                             cGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGt                                                                     >  2:1299402/1‑72 (MQ=255)

                                                                                             cGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGt                                                                     <  1:1299402/72‑1 (MQ=255)

                                                                                             cGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGcc                                            >  2:898639/1‑97 (MQ=255)

                                                                                             cGTAAGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGt  >  1:213417/1‑139 (MQ=255)

GTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGT  >  NC_000913/4614163‑4614394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A